{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:45:51Z","timestamp":1785901551754,"version":"3.56.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Vet Scand"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The <jats:italic>blaZ<\/jats:italic> gene encoding penicillin resistance can be located either chromosomally or on plasmids. The aim of this study was to investigate the genetic relationships and to determine the location of the <jats:italic>blaZ<\/jats:italic> gene in <jats:italic>S. aureus<\/jats:italic> isolated in bovine mastitis in Finland and Sweden.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Seventy-eight \u03b2-lactamase positive <jats:italic>S. aureus<\/jats:italic> isolates from bovine mastitis (34 from Finland and 44 from Sweden) were included in the study. The localization of <jats:italic>blaZ<\/jats:italic> gene was determined by Southern blotting. The <jats:italic>blaZ<\/jats:italic> genes of the isolates were sequenced and the sequences were translated to beta-lactamase proteins and further grouped as different protein signatures. The isolates and, as control, 33 Swedish and 36 Finnish beta-lactamase negative isolates were typed with pulsed-field gel electrophoresis (PFGE).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In 26 out of 34 Finnish isolates (76.5%) and in 25 out of 44 Swedish isolates (56.8%) the <jats:italic>blaZ<\/jats:italic> gene was localized on a plasmid. Six different protein signatures were found. One signature was found only in four Swedish isolates, but all other signatures were found both in Finnish and Swedish isolates. The PFGE results revealed a diversity of <jats:italic>S. aureus<\/jats:italic> clones. The protein signatures were not clearly associated with certain pulsotypes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The plasmid location of the <jats:italic>blaZ<\/jats:italic> gene was not statistically significantly more common in Finland than in Sweden, and hence does not explain the higher proportion of penicillin-resistant isolates of <jats:italic>S. aureus<\/jats:italic> causing bovine mastitis in Finland compared to Sweden.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1751-0147-54-69","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T11:25:50Z","timestamp":1353669950000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis"],"prefix":"10.1186","volume":"54","author":[{"given":"Arzu Funda","family":"Bagcigil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suvi","family":"Taponen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Koort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Bengtsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Liisa","family":"Myllyniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satu","family":"Py\u00f6r\u00e4l\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,11,23]]},"reference":[{"key":"838_CR1","doi-asserted-by":"publisher","first-page":"1877","DOI":"10.3168\/jds.S0022-0302(06)72256-1","volume":"89","author":"H Barkema","year":"2006","unstructured":"Barkema H, Schukken YH, Zadoks RN: Invited review: the role of cow, pathogen, and treatment regimen in the therapeutic success of bovine staphylococcus aureus mastitis. J Dairy Sci. 2006, 89: 1877-1895. 10.3168\/jds.S0022-0302(06)72256-1.","journal-title":"J Dairy Sci"},{"key":"838_CR2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/9781444302639.ch9","volume-title":"Antimicrobial Use in Animals","author":"P Constable","year":"2008","unstructured":"Constable P, Py\u00f6r\u00e4l\u00e4 S, Smith G: Guide for antimicrobial use in cattle. Antimicrobial Use in Animals. Edited by: Guardabassi L, Jensen LB, Kruse H. 2008, Singapore: Blackwell Publishing Ltd, Fabulous Printers PTE Ltd, 143-160."},{"key":"838_CR3","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/1751-0147-50-28","volume":"50","author":"RS Hendriksen","year":"2008","unstructured":"Hendriksen RS, Mevius DJ, Schroeter A, Teale C, Meunier D, Butaye P, Franco A, Utinane A, Amado A, Moreno M, Greko C, St\u00e4rk K, Berghold C, Myllyniemi A-L, Wasyl D, Sunde M, Aarestrup FM: Prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different European countries: 2002\u20132004. Acta Vet Scand. 2008, 50: 28-10.1186\/1751-0147-50-28.","journal-title":"Acta Vet Scand"},{"key":"838_CR4","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.3168\/jds.S0022-0302(06)72167-1","volume":"89","author":"O \u00d8ster\u00e5s","year":"2006","unstructured":"\u00d8ster\u00e5s O, S\u00f8lver\u00f8d L, Reksen O: Milk culture results in a large Norwegian survey - effects of season, parity, days in milk, resistance and clustering. J Dairy Sci. 2006, 89: 1010-1023. 10.3168\/jds.S0022-0302(06)72167-1.","journal-title":"J Dairy Sci"},{"key":"838_CR5","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.vetmic.2008.10.024","volume":"136","author":"B Bengtsson","year":"2009","unstructured":"Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Ost M, Waller KP: Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol. 2009, 136: 142-149. 10.1016\/j.vetmic.2008.10.024.","journal-title":"Vet Microbiol"},{"key":"838_CR6","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/1751-0147-53-36","volume":"53","author":"Y Persson","year":"2011","unstructured":"Persson Y, Nyman A-KJ, Gr\u00f6nlund-Andersson U: Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Vet Scand. 2011, 53: 36-10.1186\/1751-0147-53-36.","journal-title":"Acta Vet Scand"},{"key":"838_CR7","doi-asserted-by":"publisher","first-page":"1921","DOI":"10.3168\/jds.2011-5065","volume":"95","author":"V Saini","year":"2012","unstructured":"Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW: Herd-level association between antimicrobial use and antimicrobial resistance in bovine mastitis staphylococcus aureus isolates on Canadian dairy farms. J Dairy Sci. 2012, 95: 1921-1929. 10.3168\/jds.2011-5065.","journal-title":"J Dairy Sci"},{"key":"838_CR8","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1751-0147-53-4","volume":"53","author":"P Kalmus","year":"2011","unstructured":"Kalmus P, Aasm\u00e4e B, K\u00e4rssin A, Orro T, Kask K: Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet Scand. 2011, 53: 4-10.1186\/1751-0147-53-4.","journal-title":"Acta Vet Scand"},{"key":"838_CR9","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1089\/fpd.2010.0661","volume":"8","author":"H-M Nam","year":"2011","unstructured":"Nam H-M, Lee A-L, Jung M-NK, Jang G-C, Wee S-H, Lim S-K: Antimicrobial susceptibility of staphylococus aureus and characterization of methicillin-resistant staphylococcus aureus isolates from bovine mastitis in Korea. Foodborne Pathog Dis. 2011, 8: 231-238. 10.1089\/fpd.2010.0661.","journal-title":"Foodborne Pathog Dis"},{"key":"838_CR10","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.3168\/jds.S0022-0302(04)73366-4","volume":"87","author":"A Pitk\u00e4l\u00e4","year":"2004","unstructured":"Pitk\u00e4l\u00e4 A, Haveri M, Py\u00f6r\u00e4l\u00e4 S, Myllys V, Honkanen-Buzalski T: Bovine mastitis in Finland \u2013 prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci. 2004, 87: 2433-2441. 10.3168\/jds.S0022-0302(04)73366-4.","journal-title":"J Dairy Sci"},{"key":"838_CR11","first-page":"363","volume":"110","author":"M Nevala","year":"2004","unstructured":"Nevala M, Taponen S, Py\u00f6r\u00e4l\u00e4 S: Bacterial etiology of bovine clinical mastitis \u2013 data from saari ambulatory clinic in 2002\u20132003. Suomen El\u00e4inl\u00e4\u00e4k\u00e4rilehti (Finnish Vet J). 2004, 110: 363-369.","journal-title":"Suomen El\u00e4inl\u00e4\u00e4k\u00e4rilehti (Finnish Vet J)"},{"key":"838_CR12","unstructured":"FinRes-Vet 2005\u20132006: Finnish veterinary antimicrobial resistance monitoring and consumption of antimicrobial agents.http:\/\/www.evira.fi\/uploads\/WebShopFiles\/1198141211941.pdf,"},{"key":"838_CR13","first-page":"207","volume-title":"Staphylococcus molecular genetics","author":"MG Pinho","year":"2008","unstructured":"Pinho MG: Mechanisms of \u03b2-lactam and glycopeptides resistance in Staphylococcus aureus. Staphylococcus molecular genetics. Edited by: Lindsay JA. 2008, Norfolk, UK: Caister Academic Press, Norwich, 207-226."},{"key":"838_CR14","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1128\/mr.51.1.88-134.1987","volume":"51","author":"BR Lyon","year":"1987","unstructured":"Lyon BR, Skurray RA: Antimicrobial resistance of staphylococcus aureus: genetic basis. Microbiol Rev. 1987, 51: 88-134.","journal-title":"Microbiol Rev"},{"key":"838_CR15","first-page":"187","volume-title":"Antimicrobial resistance in bacteria of animal origin","author":"FM Aarestrup","year":"2006","unstructured":"Aarestrup FM, Schwarz S: Antimicrobial resistance in staphylococci and streptococci of animal origin. Antimicrobial resistance in bacteria of animal origin. Edited by: Aarestrup FM. 2006, Washington, DC: ASM Press, 187-212."},{"key":"838_CR16","doi-asserted-by":"publisher","first-page":"3057","DOI":"10.1007\/s00018-010-0389-4","volume":"67","author":"N Malachowa","year":"2010","unstructured":"Malachowa N, DeLeo FR: Mobile genetic elements of Staphylococcus aureus. Cell Mol Life Sci. 2010, 67: 3057-3071. 10.1007\/s00018-010-0389-4.","journal-title":"Cell Mol Life Sci"},{"key":"838_CR17","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1186\/BF03547026","volume":"40","author":"M Vesterholm-Nielsen","year":"1999","unstructured":"Vesterholm-Nielsen M, \u00d8lhom Larsen M, Olsen JE, Aarestrup FM: Occurrence of the blaZ gene in penicillin resistant Staphylococcus aureus isolated from bovine mastitis in Denmark. Acta Vet Scand. 1999, 40: 279-286.","journal-title":"Acta Vet Scand"},{"key":"838_CR18","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1093\/jac\/dki492","volume":"57","author":"JE Olsen","year":"2006","unstructured":"Olsen JE, Christensen H, Aarestrup FM: Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci. J Antimicrob Chemother. 2006, 57: 450-460. 10.1093\/jac\/dki492.","journal-title":"J Antimicrob Chemother"},{"key":"838_CR19","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.vetmic.2007.02.012","volume":"123","author":"S Malik","year":"2007","unstructured":"Malik S, Christensen H, Peng H, Barton MD: Presence and diversity of the \u03b2-lactamase gene in cat and dog staphylococci. Vet Microbiol. 2007, 123: 162-168. 10.1016\/j.vetmic.2007.02.012.","journal-title":"Vet Microbiol"},{"key":"838_CR20","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1128\/jcm.30.7.1654-1660.1992","volume":"30","author":"OG Brakstad","year":"1992","unstructured":"Brakstad OG, Aasbakk K, Maeland JA: Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol. 1992, 30: 1654-1660.","journal-title":"J Clin Microbiol"},{"key":"838_CR21","volume-title":"Molecular cloning. A laborafory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T: Molecular cloning. A laborafory manual. 1989, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2","edition":"2"},{"key":"838_CR22","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s100960050438","volume":"19","author":"S Salmenlinna","year":"2000","unstructured":"Salmenlinna S, Lyytik\u00e4inen O, Kotilainen P, Scotford R, Siren E, Vuopio-Varkila J: Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Finland. Eur J Clin Microbiol Infect Dis. 2000, 19: 101-107. 10.1007\/s100960050438.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"838_CR23","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1128\/AAC.40.5.1170","volume":"40","author":"JF Tomayko","year":"1996","unstructured":"Tomayko JF, Zscheck KK, Singh KV, Murray BE: Comparison of the beta-lactamase gene cluster in clonally distinct strains of Enterococcus faecalis. Antimicrob Agents Chemother. 1996, 40: 1170-1174.","journal-title":"Antimicrob Agents Chemother"},{"key":"838_CR24","doi-asserted-by":"publisher","first-page":"2381","DOI":"10.1534\/genetics.106.069443","volume":"176","author":"DC Marciano","year":"2007","unstructured":"Marciano DC, Karkouti OY, Palzkill T: A fitness cost associated with the antibiotic resistance enzyme SME-1 beta-lactamase. Genetics. 2007, 176: 2381-2392. 10.1534\/genetics.106.069443.","journal-title":"Genetics"},{"key":"838_CR25","doi-asserted-by":"publisher","first-page":"4551","DOI":"10.3168\/jds.S0022-0302(06)72504-8","volume":"89","author":"KL Anderson","year":"2006","unstructured":"Anderson KL, Lyman RL: Long-term persistence of specific genetic types of mastitis causing Staphylococcus aureus on three dairies. J Dairy Sci. 2006, 89: 4551-4556. 10.3168\/jds.S0022-0302(06)72504-8.","journal-title":"J Dairy Sci"},{"key":"838_CR26","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1128\/CMR.8.4.557","volume":"8","author":"DM Livermore","year":"1995","unstructured":"Livermore DM: \u03b2-lactamases in laboratory and clinical resistance. Clin Microbiol Reviews. 1995, 8: 557-584.","journal-title":"Clin Microbiol Reviews"},{"key":"838_CR27","volume-title":"Nordiska riktlinjer f\u00f6r mastitbehandling (Nordic guidelines for mastitis treatment)","author":"NMSM (Nordiske Meieriorganisasjoners Samarbeidsutvalg for Mjolkekvalitetsarbeid)","year":"2009","unstructured":"NMSM (Nordiske Meieriorganisasjoners Samarbeidsutvalg for Mjolkekvalitetsarbeid): Meeting of the Nordic Dairy Association's Committee for Milk Quality, 12th June 2009, Lund, Sweden. Nordiska riktlinjer f\u00f6r mastitbehandling (Nordic guidelines for mastitis treatment). 2009,http:\/\/www.sva.se\/upload\/Redesign2011\/Pdf\/antibiotika\/Nordiska_riktlinjer_for_mastitbehandling_lagupplost.pdf,"},{"key":"838_CR28","first-page":"85","volume-title":"Use of intramammary antimicrobials in Finland - towards lower consumption and targeted treatment of mastitis","author":"S Py\u00f6r\u00e4l\u00e4","year":"2012","unstructured":"Py\u00f6r\u00e4l\u00e4 S, Kaartinen L, Simojoki H, Kivilahti-M\u00e4ntyl\u00e4 K: Use of intramammary antimicrobials in Finland - towards lower consumption and targeted treatment of mastitis. 2012, Lisbon, Portugal: XXVII World Buiatrics Congress, 85-Abstracts, OC 14"},{"key":"838_CR29","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0167-5877(99)00057-4","volume":"42","author":"T Grave","year":"1999","unstructured":"Grave T, Greko C, Nilsson L, Odensvik K, M\u00f8rk T, R\u00f8nning M: The usage of veterinary antibacterial drugs for mastitis in cattle in Norway and Sweden during 1990\u20131997. Prev Vet Med. 1999, 42: 45-55. 10.1016\/S0167-5877(99)00057-4.","journal-title":"Prev Vet Med"},{"key":"838_CR30","doi-asserted-by":"publisher","first-page":"262","DOI":"10.3168\/jds.S0022-0302(07)72627-9","volume":"90","author":"M Pol","year":"2007","unstructured":"Pol M, Ruegg PL: Relationship between antimicrobial drug usage and antimicrobial susceptibility of gram-positive mastitis pathogens. J Dairy Sci. 2007, 90: 262-273. 10.3168\/jds.S0022-0302(07)72627-9.","journal-title":"J Dairy Sci"},{"key":"838_CR31","unstructured":"FinRes-Vet 1999: Bacterial Resistance to Antimicrobial Agents in Finland.http:\/\/wwwb.mmm.fi\/el\/julk\/finres99en.html,"},{"key":"838_CR32","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1128\/JCM.43.2.959-961.2005","volume":"43","author":"M Haveri","year":"2005","unstructured":"Haveri M, Taponen S, Vuopio-Varkila J, Salmenlinna S, Py\u00f6r\u00e4l\u00e4 S: Bacterial genotype affects the manifestation and persistence of bovine Staphylococcus aureus intramammary infection. J Clin Microbiol. 2005, 43: 959-961. 10.1128\/JCM.43.2.959-961.2005.","journal-title":"J Clin Microbiol"},{"key":"838_CR33","doi-asserted-by":"publisher","first-page":"2550","DOI":"10.3168\/jds.2009-2971","volume":"93","author":"BHP van den Borne","year":"2010","unstructured":"van den Borne BHP, Nielen M, van Schaik G, Melchior MB, Lam TJGM, Zadoks RN: Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment. J Dairy Sci. 2010, 93: 2550-2558. 10.3168\/jds.2009-2971.","journal-title":"J Dairy Sci"},{"key":"838_CR34","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1186\/1751-0147-44-53","volume":"44","author":"S Taponen","year":"2003","unstructured":"Taponen S, Jantunen A, Py\u00f6r\u00e4l\u00e4 E, Py\u00f6r\u00e4l\u00e4 S: Efficacy of targeted 5-day combined parenteral and intramammary treatment of clinical mastitis caused by penicillin-susceptible or penicillin-resistant Staphylococcus aureus. Acta Vet Scand. 2003, 44: 53-62. 10.1186\/1751-0147-44-53.","journal-title":"Acta Vet Scand"},{"key":"838_CR35","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S0378-1135(03)00186-X","volume":"97","author":"J Vintov","year":"2003","unstructured":"Vintov J, Aarestrup FM, Zinn CE, Olsen JE: Phage types and antimicrobial resistance among Danish bovine Staphylococcus aureus isolates since the 1950s. Vet Microbiol. 2003, 97: 63-72. 10.1016\/S0378-1135(03)00186-X.","journal-title":"Vet Microbiol"},{"key":"838_CR36","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1017\/S095026880200715X","volume":"129","author":"S Waage","year":"2002","unstructured":"Waage S, Bjorland J, Caugant DA, Oppegaard H, Tollersrud T, M\u00f8rk T, Aarestrup FM: Spread of Staphylococcus aureus resistant to penicillin and tetracycline within and between dairy herds. Epidemiol Infect. 2002, 129: 193-202.","journal-title":"Epidemiol Infect"}],"container-title":["Acta Veterinaria Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1751-0147-54-69.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T22:06:38Z","timestamp":1630533998000},"score":1,"resource":{"primary":{"URL":"https:\/\/actavetscand.biomedcentral.com\/articles\/10.1186\/1751-0147-54-69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,23]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["838"],"URL":"https:\/\/doi.org\/10.1186\/1751-0147-54-69","relation":{},"ISSN":["1751-0147"],"issn-type":[{"value":"1751-0147","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,23]]},"assertion":[{"value":"7 September 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 November 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 November 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"69"}}