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These approaches were tested and compared using data from the thermophilic, phototrophic mat communities from Octopus and Mushroom Springs in Yellowstone National Park (USA). The models were based on three distinct microbial guilds: oxygenic phototrophs, filamentous anoxygenic phototrophs, and sulfate-reducing bacteria. Two phases, day and night, were modeled to account for differences in the sources of mass and energy and the routes available for their exchange.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The <jats:italic>in silico<\/jats:italic> models were used to explore fundamental questions in ecology including the prediction of and explanation for measured relative abundances of primary producers in the mat, theoretical tradeoffs between overall productivity and the generation of toxic by-products, and the relative robustness of various guild interactions.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The three modeling approaches represent a flexible toolbox for creating cellular metabolic networks to study microbial communities on scales ranging from cells to ecosystems. A comparison of the three methods highlights considerations for selecting the one most appropriate for a given microbial system. For instance, communities represented only by metagenomic data can be modeled using the pooled method which analyzes a community's total metabolic potential without attempting to partition enzymes to different organisms. Systems with extensive <jats:italic>a priori<\/jats:italic> information on microbial guilds can be represented using the compartmentalized technique, employing distinct control volumes to separate guild-appropriate enzymes and metabolites. If the complexity of a compartmentalized network creates an unacceptable computational burden, the nested analysis approach permits greater scalability at the cost of more user intervention through multiple rounds of pathway analysis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-3-114","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T07:18:49Z","timestamp":1260429529000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":95,"title":["In silico approaches to study mass and energy flows in microbial consortia: a syntrophic case study"],"prefix":"10.1186","volume":"3","author":[{"given":"Reed","family":"Taffs","sequence":"first","affiliation":[]},{"given":"John E","family":"Aston","sequence":"additional","affiliation":[]},{"given":"Kristen","family":"Brileya","sequence":"additional","affiliation":[]},{"given":"Zackary","family":"Jay","sequence":"additional","affiliation":[]},{"given":"Christian G","family":"Klatt","sequence":"additional","affiliation":[]},{"given":"Shawn","family":"McGlynn","sequence":"additional","affiliation":[]},{"given":"Natasha","family":"Mallette","sequence":"additional","affiliation":[]},{"given":"Scott","family":"Montross","sequence":"additional","affiliation":[]},{"given":"Robin","family":"Gerlach","sequence":"additional","affiliation":[]},{"given":"William P","family":"Inskeep","sequence":"additional","affiliation":[]},{"given":"David M","family":"Ward","sequence":"additional","affiliation":[]},{"given":"Ross P","family":"Carlson","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,12,10]]},"reference":[{"issue":"5879","key":"382_CR1","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1126\/science.1153213","volume":"320","author":"PG Falkowski","year":"2008","unstructured":"Falkowski PG, Fenchel T, Delong EF: The microbial engines that drive Earth's biogeochemical cycles. 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