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Genome-scale modeling of bacterial metabolic networks provides a powerful tool to identify and analyze pathways required for successful intracellular replication during host-pathogen interaction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have developed and validated a genome-scale metabolic network of <jats:italic>Salmonella typhimurium<\/jats:italic> LT2 (iRR1083). This model accounts for 1,083 genes that encode proteins catalyzing 1,087 unique metabolic and transport reactions in the bacterium. We employed flux balance analysis and <jats:italic>in silico<\/jats:italic> gene essentiality analysis to investigate growth under a wide range of conditions that mimic <jats:italic>in vitro<\/jats:italic> and host cell environments. Gene expression profiling of <jats:italic>S. typhimurium<\/jats:italic> isolated from macrophage cell lines was used to constrain the model to predict metabolic pathways that are likely to be operational during infection.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Our analysis suggests that there is a robust minimal set of metabolic pathways that is required for successful replication of <jats:italic>Salmonella<\/jats:italic> inside the host cell. This model also serves as platform for the integration of high-throughput data. Its computational power allows identification of networked metabolic pathways and generation of hypotheses about metabolism during infection, which might be used for the rational design of novel antibiotics or vaccine strains.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-3-38","type":"journal-article","created":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T18:13:52Z","timestamp":1239214432000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":137,"title":["Constraint-based analysis of metabolic capacity of Salmonella typhimurium during host-pathogen interaction"],"prefix":"10.1186","volume":"3","author":[{"given":"Anu","family":"Raghunathan","sequence":"first","affiliation":[]},{"given":"Jennifer","family":"Reed","sequence":"additional","affiliation":[]},{"given":"Sookil","family":"Shin","sequence":"additional","affiliation":[]},{"given":"Bernhard","family":"Palsson","sequence":"additional","affiliation":[]},{"given":"Simon","family":"Daefler","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,4,8]]},"reference":[{"key":"306_CR1","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1038\/nrmicro1788","volume":"6","author":"A Haraga","year":"2008","unstructured":"Haraga A, Ohlson MB, Miller SI: Salmonellae interplay with host cells. 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