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We have developed a novel method for this integration, and applied it to genome-wide data from multiple Arabidopsis microarray experiments performed under a variety of experimental conditions. The goal of this study is to identify functional globally co-regulated gene modules in the Arabidopsis genome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Following the analysis of 21,000 Arabidopsis genes in 43 datasets and about 2 \u00d7 10<jats:sup>8<\/jats:sup>gene pairs, we identified a globally co-expressed gene network. We found clusters of globally co-expressed Arabidopsis genes that are enriched for known Gene Ontology annotations. Two types of modules were identified in the regulatory network that differed in their sensitivity to the node-scoring parameter; we further showed these two pertain to general and specialized modules. Some of these modules were further investigated using the<jats:italic>Genevestigator<\/jats:italic>compendium of microarray experiments. Analyses of smaller subsets of data lead to the identification of condition-specific modules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our method for identification of gene clusters allows the integration of diverse microarray experiments from many sources. The analysis reveals that part of the<jats:italic>Arabidopsis<\/jats:italic>transcriptome is globally co-expressed, and can be further divided into known as well as novel functional gene modules. Our methodology is general enough to apply to any set of microarray experiments, using any scoring function.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1752-0509-3-86","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T18:14:10Z","timestamp":1252001650000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Large-scale analysis of Arabidopsis transcription reveals a basal co-regulation network"],"prefix":"10.1186","volume":"3","author":[{"given":"Osnat","family":"Atias","sequence":"first","affiliation":[]},{"given":"Benny","family":"Chor","sequence":"additional","affiliation":[]},{"given":"Daniel A","family":"Chamovitz","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2009,9,3]]},"reference":[{"issue":"25","key":"354_CR1","doi-asserted-by":"publisher","first-page":"14863","DOI":"10.1073\/pnas.95.25.14863","volume":"95","author":"MB Eisen","year":"1998","unstructured":"Eisen MB, Spellman PT, Brown PO, Botstein D: Cluster analysis and display of genome-wide expression patterns. 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