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While evidence for effect on genes associated with energy production were seen, an ontological analysis also indicated an effect of Cd exposure on DNA integrity and transcriptional activity. DEBtox modelling showed an effect of FA on costs for growth and reproduction (i.e. for production of new and differentiated biomass). The microarray analysis supported this effect, showing an effect of FA on protein integrity and turnover that would be expected to have consequences for rates of somatic growth. For AZ, the physiological mode of action predicted by DEBtox was increased cost for maintenance. The transcriptional analysis demonstrated that this increase resulted from effects on DNA integrity as indicated by changes in the expression of genes chromosomal repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results have established that outputs from process based models and transcriptomics analyses can help to link mechanisms of action of toxic chemicals with resulting demographic effects. Such complimentary analyses can assist in the categorisation of chemicals for risk assessment purposes.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1752-0509-4-32","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T19:15:01Z","timestamp":1269371701000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans"],"prefix":"10.1186","volume":"4","author":[{"given":"Suresh","family":"Swain","sequence":"first","affiliation":[]},{"given":"Jodie F","family":"Wren","sequence":"additional","affiliation":[]},{"given":"Stephen R","family":"St\u00fcrzenbaum","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Kille","sequence":"additional","affiliation":[]},{"given":"A John","family":"Morgan","sequence":"additional","affiliation":[]},{"given":"Tjalling","family":"Jager","sequence":"additional","affiliation":[]},{"given":"Martijs J","family":"Jonker","sequence":"additional","affiliation":[]},{"given":"Peter K","family":"Hankard","sequence":"additional","affiliation":[]},{"given":"Claus","family":"Svendsen","sequence":"additional","affiliation":[]},{"given":"Jenifer","family":"Owen","sequence":"additional","affiliation":[]},{"given":"B Ann","family":"Hedley","sequence":"additional","affiliation":[]},{"given":"Mark","family":"Blaxter","sequence":"additional","affiliation":[]},{"given":"David J","family":"Spurgeon","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,3,23]]},"reference":[{"key":"421_CR1","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1006\/anbe.2003.2122","volume":"66","author":"AI Barnes","year":"2003","unstructured":"Barnes AI, Partridge L: Costing reproduction. 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