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Also, some of these methods focus on specific pathways, and thus are limited to expansion to the whole metabolism.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In the present study, we propose a system framework employing a retrosynthesis model with a prioritization scoring algorithm. This new strategy allows deducing the novel promising pathways for the synthesis of a desired chemical together with information on enzymes involved based on structural changes and reaction mechanisms present in the system database. The prioritization scoring algorithm employing Tanimoto coefficient and group contribution method allows examination of structurally qualified pathways to recognize which pathway is more appropriate. In addition, new concepts of binding site covalence, estimation of pathway distance and organism specificity were taken into account to identify the best synthetic pathway. Parameters of these factors can be evolutionarily optimized when a newly proven synthetic pathway is registered. As the proofs of concept, the novel synthetic pathways for the production of isobutanol, 3-hydroxypropionate, and butyryl-CoA were predicted. The prediction shows a high reliability, in which experimentally verified synthetic pathways were listed within the top 0.089% of the identified pathway candidates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>It is expected that the system framework developed in this study would be useful for the <jats:italic>in silico<\/jats:italic> design of novel metabolic pathways to be employed for the efficient production of chemicals, fuels and materials.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-4-35","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T06:13:53Z","timestamp":1269929633000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":117,"title":["Prediction of novel synthetic pathways for the production of desired chemicals"],"prefix":"10.1186","volume":"4","author":[{"given":"Ayoun","family":"Cho","sequence":"first","affiliation":[]},{"given":"Hongseok","family":"Yun","sequence":"additional","affiliation":[]},{"given":"Jin Hwan","family":"Park","sequence":"additional","affiliation":[]},{"given":"Sang Yup","family":"Lee","sequence":"additional","affiliation":[]},{"given":"Sunwon","family":"Park","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,3,28]]},"reference":[{"key":"424_CR1","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S0928-4869(00)00006-9","volume":"8","author":"M Arita","year":"2000","unstructured":"Arita M: Metabolic reconstruction using shortest paths. 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