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The ability of <jats:italic>R. sphaeroides<\/jats:italic> to produce hydrogen (H<jats:sub>2<\/jats:sub>), polyhydroxybutyrate (PHB) or other hydrocarbons, as well as its ability to utilize atmospheric carbon dioxide (CO<jats:sub>2<\/jats:sub>) as a carbon source under defined conditions, make it an excellent candidate for use in a wide variety of biotechnological applications. A genome-level understanding of its metabolic capabilities should help realize this biotechnological potential.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Here we present a genome-scale metabolic network model for <jats:italic>R. sphaeroides<\/jats:italic> strain 2.4.1, designated iRsp1095, consisting of 1,095 genes, 796 metabolites and 1158 reactions, including <jats:italic>R. sphaeroides<\/jats:italic>-specific biomass reactions developed in this study. Constraint-based analysis showed that iRsp1095 agreed well with experimental observations when modeling growth under respiratory and phototrophic conditions. Genes essential for phototrophic growth were predicted by single gene deletion analysis. During pathway-level analyses of <jats:italic>R. sphaeroides<\/jats:italic> metabolism, an alternative route for CO<jats:sub>2<\/jats:sub> assimilation was identified. Evaluation of photoheterotrophic H<jats:sub>2<\/jats:sub> production using iRsp1095 indicated that maximal yield would be obtained from growing cells, with this predicted maximum ~50% higher than that observed experimentally from wild type cells. Competing pathways that might prevent the achievement of this theoretical maximum were identified to guide future genetic studies.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>iRsp1095 provides a robust framework for future metabolic engineering efforts to optimize the solar- and nutrient-powered production of biofuels and other valuable products by <jats:italic>R. sphaeroides<\/jats:italic> and closely related organisms.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-5-116","type":"journal-article","created":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T06:18:27Z","timestamp":1311315507000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["iRsp1095: A genome-scale reconstruction of the Rhodobacter sphaeroides metabolic network"],"prefix":"10.1186","volume":"5","author":[{"given":"Saheed","family":"Imam","sequence":"first","affiliation":[]},{"given":"Safak","family":"Yilmaz","sequence":"additional","affiliation":[]},{"given":"Ugur","family":"Sohmen","sequence":"additional","affiliation":[]},{"given":"Alexander S","family":"Gorzalski","sequence":"additional","affiliation":[]},{"given":"Jennifer L","family":"Reed","sequence":"additional","affiliation":[]},{"given":"Daniel R","family":"Noguera","sequence":"additional","affiliation":[]},{"given":"Timothy J","family":"Donohue","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,7,21]]},"reference":[{"key":"716_CR1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-8815-5","volume-title":"The Purple Phototrophic Bacteria","author":"CN Hunter","year":"2009","unstructured":"Hunter CN, Daldal F, Thurnauer MC, Beatty JT: The Purple Phototrophic Bacteria. 2009, 28: Springer,"},{"issue":"12","key":"716_CR2","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1038\/nbt.1586","volume":"27","author":"S Atsumi","year":"2009","unstructured":"Atsumi S, Higashide W, Liao JC: Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. 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