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This manually-curated reconstruction accounts for 584 genes, 1386 reactions, and 1411 metabolites. By using flux balance analysis, the model was extensively validated against literature data on the <jats:italic>C. salexigens<\/jats:italic> phenotypic features, the transport and use of different substrates for growth as well as against experimental observations on the uptake and accumulation of industrially important organic osmolytes, ectoine, betaine, and its precursor choline, which play important roles in the adaptive response to osmotic stress.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This work presents the first comprehensive genome-scale metabolic model of a halophilic bacterium. Being a useful guide for identification and filling of knowledge gaps, the reconstructed metabolic network <jats:italic>i<\/jats:italic> OA584 will accelerate the research on halophilic bacteria towards application of systems biology approaches and design of metabolic engineering strategies.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-5-12","type":"journal-article","created":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T07:16:27Z","timestamp":1297149387000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Genome-scale reconstruction of metabolic network for a halophilic extremophile, Chromohalobacter salexigens DSM 3043"],"prefix":"10.1186","volume":"5","author":[{"given":"\u00d6zlem","family":"Ates","sequence":"first","affiliation":[]},{"given":"Ebru Toksoy","family":"Oner","sequence":"additional","affiliation":[]},{"given":"Kazim Y","family":"Arga","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2011,1,21]]},"reference":[{"key":"602_CR1","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1128\/jb.127.3.1550-1557.1976","volume":"127","author":"A Chien","year":"1976","unstructured":"Chien A, Edgar DB, Trela JM: Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. 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