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By following a systematic workflow we reconstruct a genome-scale metabolic model for <jats:italic>M. acetivorans<\/jats:italic>. This process relies on previously developed computational tools developed in our group to correct growth prediction inconsistencies with <jats:italic>in vivo<\/jats:italic> data sets and rectify topological inconsistencies in the model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The generated model <jats:italic>i<\/jats:italic> VS941 accounts for 941 genes, 705 reactions and 708 metabolites. The model achieves 93.3% prediction agreement with <jats:italic>in vivo<\/jats:italic> growth data across different substrates and multiple gene deletions. The model also correctly recapitulates metabolic pathway usage patterns of <jats:italic>M. acetivorans<\/jats:italic> such as the indispensability of flux through methanogenesis for growth on acetate and methanol and the unique biochemical characteristics under growth on carbon monoxide.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Based on the size of the genome-scale metabolic reconstruction and extent of validated predictions this model represents the most comprehensive up-to-date effort to catalogue methanogenic metabolism. The reconstructed model is available in spreadsheet and SBML formats to enable dissemination.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-5-28","type":"journal-article","created":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T20:09:20Z","timestamp":1297886960000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans"],"prefix":"10.1186","volume":"5","author":[{"given":"Vinay","family":"Satish Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James G","family":"Ferry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Costas D","family":"Maranas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,2,15]]},"reference":[{"issue":"2","key":"620_CR1","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/nrmicro1949","volume":"7","author":"AM Feist","year":"2009","unstructured":"Feist AM, et al., et al.: Reconstruction of biochemical networks in microorganisms. 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