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In this article, we developed and validated a genome-scale dynamic flux balance model, using experimentally determined kinetic constraints.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Appropriate equations for maintenance, biomass composition, anaerobic metabolism and nutrient uptake are key to improve model performance, especially for predicting glycerol and ethanol synthesis. Prediction profiles of synthesis and consumption of the main metabolites involved in alcoholic fermentation closely agreed with experimental data obtained from numerous lab and industrial fermentations under different environmental conditions. Finally, fermentation simulations of genetically engineered yeasts closely reproduced previously reported experimental results regarding final concentrations of the main fermentation products such as ethanol and glycerol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A useful tool to describe, understand and predict metabolite production in batch yeast cultures was developed. The resulting model, if used wisely, could help to search for new metabolic engineering strategies to manage ethanol content in batch fermentations.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1752-0509-5-75","type":"journal-article","created":{"date-parts":[[2011,5,19]],"date-time":"2011-05-19T19:22:09Z","timestamp":1305832929000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Expanding a dynamic flux balance model of yeast fermentation to genome-scale"],"prefix":"10.1186","volume":"5","author":[{"given":"Felipe A","family":"Vargas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Pizarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Ricardo","family":"P\u00e9rez-Correa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Agosin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,5,19]]},"reference":[{"key":"666_CR1","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1007\/s00253-006-0610-4","volume":"73","author":"QX Kong","year":"2007","unstructured":"Kong QX, Cao LM, Zhang AL, Chen X: Overexpressing GLT1 in gpd1 mutant to improve the production of ethanol of Saccharomyces cerevisiae. 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