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In this context, recent approaches have tended to compare experimental flux measurements with topological network analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Metabolic networks describing the main pathways of central carbon metabolism were set up for a bacteria species (<jats:italic>Corynebacterium glutamicum<\/jats:italic>) and a plant species (<jats:italic>Brassica napus<\/jats:italic>) for which experimental flux maps were available. The structural properties of each network were then studied using the concept of elementary flux modes. To do this, coefficients of flux efficiency were calculated for each reaction within the networks by using selected sets of elementary flux modes. Then the relative differences - reflecting the change of substrate<jats:italic>i.e<\/jats:italic>. a sugar source for<jats:italic>C<\/jats:italic>.<jats:italic>glutamicum<\/jats:italic>and a nitrogen source for<jats:italic>B<\/jats:italic>.<jats:italic>napus<\/jats:italic>- of both flux efficiency and flux measured experimentally were compared. For both organisms, there is a clear relationship between these parameters, thus indicating that the network structure described by the elementary flux modes had captured a significant part of the metabolic activity in both biological systems. In<jats:italic>B<\/jats:italic>.<jats:italic>napus<\/jats:italic>, the extension of the elementary flux mode analysis to an enlarged metabolic network still resulted in a clear relationship between the change in the coefficients and that of the measured fluxes. Nevertheless, the limitations of the method to fit some particular fluxes are discussed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This consistency between EFM analysis and experimental flux measurements, validated on two metabolic systems allows us to conclude that elementary flux mode analysis could be a useful tool to complement<jats:sup>13<\/jats:sup>C metabolic flux analysis, by allowing the prediction of changes in internal fluxes before carbon labelling experiments.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1752-0509-5-95","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T06:20:01Z","timestamp":1308637201000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Comparison between elementary flux modes analysis and 13C-metabolic fluxes measured in bacterial and plant cells"],"prefix":"10.1186","volume":"5","author":[{"given":"Marie","family":"Beurton-Aimar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Beauvoit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Monier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Vall\u00e9e","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Dieuaide-Noubhani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Colombi\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,6,20]]},"reference":[{"key":"707_CR1","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1007\/s00253-008-1770-1","volume":"81","author":"CT Trinh","year":"2009","unstructured":"Trinh CT, Wlaschin A, Srienc F: Elementary mode analysis: a useful metabolic pathway analysis tool for characterizing cellular metabolism. 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