{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T01:19:46Z","timestamp":1778548786806,"version":"3.51.4"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Syst Biol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Yarrowia lipolytica<\/jats:italic> is an oleaginous yeast which has emerged as an important microorganism for several biotechnological processes, such as the production of organic acids, lipases and proteases. It is also considered a good candidate for single-cell oil production. Although some of its metabolic pathways are well studied, its metabolic engineering is hindered by the lack of a genome-scale model that integrates the current knowledge about its metabolism.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Combining <jats:italic>in silico<\/jats:italic> tools and expert manual curation, we have produced an accurate genome-scale metabolic model for <jats:italic>Y. lipolytica<\/jats:italic>. Using a scaffold derived from a functional metabolic model of the well-studied but phylogenetically distant yeast <jats:italic>S. cerevisiae<\/jats:italic>, we mapped conserved reactions, rewrote gene associations, added species-specific reactions and inserted specialized copies of scaffold reactions to account for species-specific expansion of protein families. We used physiological measures obtained under lab conditions to validate our predictions.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>\n              <jats:italic>Y. lipolytica<\/jats:italic> iNL895 represents the first well-annotated metabolic model of an oleaginous yeast, providing a base for future metabolic improvement, and a starting point for the metabolic reconstruction of other species in the <jats:italic>Yarrowia<\/jats:italic> clade and other oleaginous yeasts.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-6-35","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T09:39:24Z","timestamp":1347701964000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":116,"title":["A genome-scale metabolic model of the lipid-accumulating yeast Yarrowia lipolytica"],"prefix":"10.1186","volume":"6","author":[{"given":"Nicolas","family":"Loira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Dulermo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Marc","family":"Nicaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David James","family":"Sherman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,5,4]]},"reference":[{"issue":"5","key":"920_CR1","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1007\/s00253-008-1625-9","volume":"80","author":"Q Li","year":"2008","unstructured":"Li Q, Du W, Liu D: Perspectives of microbial oils for biodiesel production. 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