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Although automatic methods for solving this problem have been previously developed, there are many situations where manual curation is still needed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We introduce a general definition of gap metabolite that allows its detection in a straightforward manner. Moreover, a method for the detection of Unconnected Modules, defined as isolated sets of blocked reactions connected through gap metabolites, is proposed. The method has been successfully applied to the curation of <jats:italic>iCG238,<\/jats:italic> the genome-scale metabolic model for the bacterium <jats:italic>Blattabacterium cuenoti<\/jats:italic>, obligate endosymbiont of cockroaches.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We found the proposed approach to be a valuable tool for the curation of genome-scale metabolic models. The outcome of its application to the genome-scale model <jats:italic>B. cuenoti iCG238<\/jats:italic> is a more accurate model version named as <jats:italic>B. cuenoti iMP240.<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-7-114","type":"journal-article","created":{"date-parts":[[2013,10,31]],"date-time":"2013-10-31T12:01:39Z","timestamp":1383220899000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Solving gap metabolites and blocked reactions in genome-scale models: application to the metabolic network of Blattabacterium cuenoti"],"prefix":"10.1186","volume":"7","author":[{"given":"Miguel","family":"Ponce-de-Le\u00f3n","sequence":"first","affiliation":[]},{"given":"Francisco","family":"Montero","sequence":"additional","affiliation":[]},{"given":"Juli","family":"Peret\u00f3","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,10,31]]},"reference":[{"key":"1197_CR1","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/nrmicro1949","volume":"7","author":"AM Feist","year":"2009","unstructured":"Feist AM, Herrg\u00e5rd MJ, Thiele I, Reed JL, Palsson B\u00d8: Reconstruction of biochemical networks in microorganisms. 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