{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T18:25:08Z","timestamp":1763663108615},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Syst Biol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In the pathogen<jats:italic>P. aeruginosa<\/jats:italic>, the formation of virulence factors is regulated via Quorum sensing signaling pathways. Due to the increasing number of strains that are resistant to antibiotics, there is a high interest to develop novel antiinfectives. In the combat of resistant bacteria, selective blockade of the bacterial cell\u2013to\u2013cell communication (Quorum sensing) has gained special interest as anti\u2013virulence strategy. Here, we modeled the<jats:italic>las<\/jats:italic>,<jats:italic>rhl<\/jats:italic>, and<jats:italic>pqs<\/jats:italic>Quorum sensing systems by a multi\u2013level logical approach to analyze how enzyme inhibitors and receptor antagonists effect the formation of autoinducers and virulence factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our rule\u2013based simulations fulfill the behavior expected from literature considering the external level of autoinducers. In the presence of PqsBCD inhibitors, the external HHQ and PQS levels are indeed clearly reduced. The magnitude of this effect strongly depends on the inhibition level. However, it seems that the pyocyanin pathway is incomplete.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>To match experimental observations we suggest a modified network topology in which PqsE and PqsR acts as receptors and an autoinducer as ligand that up\u2013regulate pyocyanin in a concerted manner. While the PQS biosynthesis is more appropriate as target to inhibit the HHQ and PQS formation, blocking the receptor PqsR that regulates the biosynthesis reduces the pyocyanin level stronger.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1752-0509-7-81","type":"journal-article","created":{"date-parts":[[2013,8,21]],"date-time":"2013-08-21T10:14:55Z","timestamp":1377080095000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Rule\u2013based regulatory and metabolic model for Quorum sensing in P. aeruginosa"],"prefix":"10.1186","volume":"7","author":[{"given":"Nadine S","family":"Schaadt","sequence":"first","affiliation":[]},{"given":"Anke","family":"Steinbach","sequence":"additional","affiliation":[]},{"given":"Rolf W","family":"Hartmann","sequence":"additional","affiliation":[]},{"given":"Volkhard","family":"Helms","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2013,8,21]]},"reference":[{"key":"1137_CR1","first-page":"149","volume":"296","author":"T Rasmussen","year":"2006","unstructured":"Rasmussen T, Givskov M, et al.: Quorum-sensing inhibitors as anti-pathogenic drugs. IJMM. 2006, 296: 149-","journal-title":"IJMM"},{"key":"1137_CR2","doi-asserted-by":"publisher","first-page":"3803","DOI":"10.1093\/emboj\/cdg366","volume":"22","author":"M Hentzer","year":"2003","unstructured":"Hentzer M, Wu H, Andersen J, Riedel K, Rasmussen T, Bagge N, Kumar N, Schembri M, Song Z, Kristoffersen P, et al.: Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO. 2003, 22: 3803-3815. 10.1093\/emboj\/cdg366.","journal-title":"EMBO"},{"key":"1137_CR3","doi-asserted-by":"publisher","first-page":"4839","DOI":"10.1128\/IAI.68.9.4839-4849.2000","volume":"68","author":"T De Kievit","year":"2000","unstructured":"De Kievit T, Iglewski B: Bacterial quorum sensing in pathogenic relationships. Infect Immun. 2000, 68: 4839-4849. 10.1128\/IAI.68.9.4839-4849.2000.","journal-title":"Infect Immun"},{"key":"1137_CR4","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1099\/mic.0.038794-0","volume":"156","author":"L Antunes","year":"2010","unstructured":"Antunes L, Ferreira R, Buckner M, Finlay B: Quorum sensing in bacterial virulence. Microbiol. 2010, 156: 2271-2282. 10.1099\/mic.0.038794-0.","journal-title":"Microbiol"},{"key":"1137_CR5","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1073\/pnas.92.5.1490","volume":"92","author":"J Pearson","year":"1995","unstructured":"Pearson J, Passador L, Iglewski B, Greenberg E: A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa. Proc Natl Acad Sci. 1995, 92: 1490-10.1073\/pnas.92.5.1490.","journal-title":"Proc Natl Acad Sci"},{"key":"1137_CR6","doi-asserted-by":"publisher","first-page":"11229","DOI":"10.1073\/pnas.96.20.11229","volume":"96","author":"E Pesci","year":"1999","unstructured":"Pesci E, Milbank J, Pearson J, McKnight S, Kende A, Greenberg E, Iglewski B: Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa. Proc Natl Acad Sci. 1999, 96: 11229-10.1073\/pnas.96.20.11229.","journal-title":"Proc Natl Acad Sci"},{"key":"1137_CR7","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1128\/jb.177.3.654-659.1995","volume":"177","author":"P Seed","year":"1995","unstructured":"Seed P, Passador L, Iglewski B: Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI: an autoinduction regulatory hierarchy. J Bacteriol. 1995, 177: 654-659.","journal-title":"J Bacteriol"},{"key":"1137_CR8","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1128\/JB.181.7.2175-2184.1999","volume":"181","author":"T De Kievit","year":"1999","unstructured":"De Kievit T, Seed P, Nezezon J, Passador L, Iglewski B: RsaL, a novel repressor of virulence gene expression in Pseudomonas aeruginosa. J Bacteriol. 1999, 181: 2175-2184.","journal-title":"J Bacteriol"},{"key":"1137_CR9","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1126\/science.8493556","volume":"260","author":"L Passador","year":"1993","unstructured":"Passador L, Cook J, Gambello M, Rust L, Iglewski B: Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science. 1993, 260: 1127-1130. 10.1126\/science.8493556.","journal-title":"Science"},{"key":"1137_CR10","doi-asserted-by":"publisher","first-page":"6424","DOI":"10.1073\/pnas.92.14.6424","volume":"92","author":"U Ochsner","year":"1995","unstructured":"Ochsner U, Reiser J: Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci. 1995, 92: 6424-10.1073\/pnas.92.14.6424.","journal-title":"Proc Natl Acad Sci"},{"key":"1137_CR11","doi-asserted-by":"crossref","first-page":"3127","DOI":"10.1128\/jb.179.10.3127-3132.1997","volume":"179","author":"E Pesci","year":"1997","unstructured":"Pesci E, Pearson J, Seed P, Iglewski B: Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J Bacteriol. 1997, 179: 3127-3132.","journal-title":"J Bacteriol"},{"key":"1137_CR12","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1046\/j.1365-2958.1997.3291701.x","volume":"24","author":"C Reimmann","year":"1997","unstructured":"Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, Haas D: The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. Mol Microbiol. 1997, 24: 309-319. 10.1046\/j.1365-2958.1997.3291701.x.","journal-title":"Mol Microbiol"},{"key":"1137_CR13","doi-asserted-by":"crossref","first-page":"3928","DOI":"10.1128\/jb.179.12.3928-3935.1997","volume":"179","author":"A Albus","year":"1997","unstructured":"Albus A, Pesci E, Runyen-Janecky L, West S, Iglewski B: Vfr controls quorum sensing in Pseudomonas aeruginosa. J Bacteriol. 1997, 179: 3928-3935.","journal-title":"J Bacteriol"},{"key":"1137_CR14","doi-asserted-by":"crossref","first-page":"19787","DOI":"10.1016\/S0021-9258(17)32089-6","volume":"269","author":"U Ochsner","year":"1994","unstructured":"Ochsner U, Fiechter A, Reiser J: Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis. J Biological Chem. 1994, 269: 19787-","journal-title":"J Biological Chem"},{"key":"1137_CR15","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1128\/JB.185.1.377-380.2003","volume":"185","author":"G Medina","year":"2003","unstructured":"Medina G, Ju\u00e1rez K, Sober\u00f3n-Ch\u00e1vez G: The Pseudomonas aeruginosa rhlAB operon is not expressed during the logarithmic phase of growth even in the presence of its activator RhlR and the autoinducer N-butyryl-homoserine lactone. J Bacteriol. 2003, 185: 377-380. 10.1128\/JB.185.1.377-380.2003.","journal-title":"J Bacteriol"},{"key":"1137_CR16","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1007\/s002530000443","volume":"54","author":"R Maier","year":"2000","unstructured":"Maier R, Soberon-Chavez G: Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Appl Microbiol Biotechnol. 2000, 54: 625-633. 10.1007\/s002530000443.","journal-title":"Appl Microbiol Biotechnol"},{"key":"1137_CR17","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1073\/pnas.0307694100","volume":"101","author":"E D\u00e9ziel","year":"2004","unstructured":"D\u00e9ziel E, L\u00e9pine F, Milot S, He J, Mindrinos M, Tompkins R, Rahme L: Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication. Proc Natl Acad Sci. 2004, 101: 1339-1344. 10.1073\/pnas.0307694100.","journal-title":"Proc Natl Acad Sci"},{"key":"1137_CR18","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1099\/mic.0.28605-0","volume":"152","author":"G Xiao","year":"2006","unstructured":"Xiao G, He J, Rahme L: Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry. Microbiol. 2006, 152: 1679-1686. 10.1099\/mic.0.28605-0.","journal-title":"Microbiol"},{"key":"1137_CR19","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1002\/cbic.201100014","volume":"12","author":"D Pistorius","year":"2011","unstructured":"Pistorius D, Ullrich A, Lucas S, Hartmann R, Kazmaier U, M\u00fcller R: Biosynthesis of 2-Alkyl-4 (1H)-Quinolones in Pseudomonas aeruginosa: Potential for therapeutic interference with pathogenicity. ChemBioChem. 2011, 12: 850-853. 10.1002\/cbic.201100014.","journal-title":"ChemBioChem"},{"key":"1137_CR20","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1111\/j.1462-2920.2010.02214.x","volume":"12","author":"G Rampioni","year":"2010","unstructured":"Rampioni G, Pustelny C, Fletcher M, Wright V, Bruce M, Rumbaugh K, Heeb S, C\u00e1mara M, Williams P: Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts. Environ Microbiol. 2010, 12: 1659-1673.","journal-title":"Environ Microbiol"},{"key":"1137_CR21","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1574-6976.2010.00247.x","volume":"35","author":"S Heeb","year":"2011","unstructured":"Heeb S, Fletcher M, Chhabra S, Diggle S, Williams P, C\u00e1mara M: Quinolones: from antibiotics to autoinducers. FEMS Microbiol Rev. 2011, 35: 247-274. 10.1111\/j.1574-6976.2010.00247.x.","journal-title":"FEMS Microbiol Rev"},{"key":"1137_CR22","doi-asserted-by":"publisher","first-page":"2752","DOI":"10.1073\/pnas.051624298","volume":"98","author":"SA Chugani","year":"2001","unstructured":"Chugani SA, Whiteley M, Lee KM, D\u2019Argenio D, Manoil C, Greenberg EP: QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa. PNAS. 2001, 98: 2752-2757. 10.1073\/pnas.051624298.","journal-title":"PNAS"},{"key":"1137_CR23","doi-asserted-by":"publisher","first-page":"3098","DOI":"10.1128\/JB.06679-11","volume":"194","author":"H Liang","year":"2012","unstructured":"Liang H, Deng X, Ji Q, Sun F, Shen T, He C: The Pseudomonas aeruginosa global regulator VqsR directly inhibits QscR to control quorum-sensing and virulence gene expression. J Bacteriol. 2012, 194: 3098-3108. 10.1128\/JB.06679-11.","journal-title":"J Bacteriol"},{"key":"1137_CR24","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1080\/01969728108927667","volume":"12","author":"H Atlan","year":"1981","unstructured":"Atlan H, Fogelman-Soulie F, Salomon J, Weisbuch G: Random boolean networks. Cybern Syst. 1981, 12: 103-121. 10.1080\/01969728108927667.","journal-title":"Cybern Syst"},{"key":"1137_CR25","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1016\/j.sigpro.2005.06.008","volume":"86","author":"H L\u00e4hdesm\u00e4ki","year":"2006","unstructured":"L\u00e4hdesm\u00e4ki H, Hautaniemi S, Shmulevich I, Yli-Harja O: Relationships between probabilistic Boolean networks and dynamic Bayesian networks as models of gene regulatory networks. Signal Process. 2006, 86: 814-834. 10.1016\/j.sigpro.2005.06.008.","journal-title":"Signal Process"},{"key":"1137_CR26","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/11885191_9","volume-title":"Comp Meth Systems Biol, Springer","author":"L Steggles","year":"2006","unstructured":"Steggles L, Banks R, Wipat A: Modelling and analysing genetic networks: From Boolean networks to Petri nets. Comp Meth Systems Biol. 2006, Springer, 127-141.4210 of Lecture Notes in Computer Science,"},{"key":"1137_CR27","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1093\/bioinformatics\/bts036","volume":"28","author":"A Rodr\u00edguez","year":"2012","unstructured":"Rodr\u00edguez A, Sosa D, Torres L, Molina B, Fr\u00edas S, Mendoza L: A Boolean network model of the FA\/BRCA pathway. Bioinformatics. 2012, 28: 858-866. 10.1093\/bioinformatics\/bts036.","journal-title":"Bioinformatics"},{"key":"1137_CR28","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1006\/jtbi.2001.2335","volume":"211","author":"L Sanchez","year":"2001","unstructured":"Sanchez L, Thieffry D: A logical analysis of the drosophila gap-gene system. J Theo Biol. 2001, 211: 115-141. 10.1006\/jtbi.2001.2335.","journal-title":"J Theo Biol"},{"key":"1137_CR29","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1039\/b910101m","volume":"5","author":"A Faur\u00e9","year":"2009","unstructured":"Faur\u00e9 A, Naldi A, Lopez F, Chaouiya C, Ciliberto A, Thieffry D: Modular logical modelling of the budding yeast cell cycle. Mol BioSyst. 2009, 5: 1787-1796. 10.1039\/b910101m.","journal-title":"Mol BioSyst"},{"key":"1137_CR30","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1006\/bulm.2000.0205","volume":"63","author":"J Dockery","year":"2001","unstructured":"Dockery J, Keener J: A mathematical model for quorum sensing in Pseudomonas aeruginosa. Bull Math Biol. 2001, 63: 95-116. 10.1006\/bulm.2000.0205.","journal-title":"Bull Math Biol"},{"key":"1137_CR31","doi-asserted-by":"publisher","first-page":"e1000819","DOI":"10.1371\/journal.pcbi.1000819","volume":"6","author":"P Melke","year":"2010","unstructured":"Melke P, Sahlin P, Levchenko A, J\u00f6nsson H: A cell-based model for quorum sensing in heterogeneous bacterial colonies. PLoS Comp Biol. 2010, 6: e1000819-10.1371\/journal.pcbi.1000819.","journal-title":"PLoS Comp Biol"},{"key":"1137_CR32","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.tcs.2006.10.012","volume":"371","author":"F Bernardini","year":"2007","unstructured":"Bernardini F, Gheorghe M, Krasnogor N: Quorum sensing P systems. Theor Comput Sci. 2007, 371: 20-33. 10.1016\/j.tcs.2006.10.012.","journal-title":"Theor Comput Sci"},{"key":"1137_CR33","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.mbs.2004.06.008","volume":"192","author":"K Anguige","year":"2004","unstructured":"Anguige K, King J, Ward J, Williams P: Mathematical modelling of therapies targeted at bacterial quorum sensing. Math Biosci. 2004, 192: 39-83. 10.1016\/j.mbs.2004.06.008.","journal-title":"Math Biosci"},{"key":"1137_CR34","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1038\/sj.jim.7000316","volume":"29","author":"D Chopp","year":"2002","unstructured":"Chopp D, Kirisits M, Moran B, Parsek M: A mathematical model of quorum sensing in a growing bacterial biofilm. J Ind Microbiol Biotech. 2002, 29: 339-346. 10.1038\/sj.jim.7000316.","journal-title":"J Ind Microbiol Biotech"},{"key":"1137_CR35","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.biosystems.2012.02.002","volume":"109","author":"J Fozard","year":"2012","unstructured":"Fozard J, Lees M, King J, Logan B: Inhibition of quorum sensing in a computational biofilm simulation. Biosystems. 2012, 109: 105-114. 10.1016\/j.biosystems.2012.02.002.","journal-title":"Biosystems"},{"key":"1137_CR36","doi-asserted-by":"crossref","first-page":"3000","DOI":"10.1128\/jb.173.9.3000-3009.1991","volume":"173","author":"M Gambello","year":"1991","unstructured":"Gambello M, Iglewski B: Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J Bacteriol. 1991, 173: 3000-3009.","journal-title":"J Bacteriol"},{"key":"1137_CR37","doi-asserted-by":"publisher","first-page":"1332","DOI":"10.1038\/ismej.2011.13","volume":"5","author":"C Wilder","year":"2011","unstructured":"Wilder C, Diggle S, Schuster M: Cooperation and cheating in Pseudomonas aeruginosa: the roles of the las, rhl and pqs quorum-sensing systems. ISME J. 2011, 5: 1332-1343. 10.1038\/ismej.2011.13.","journal-title":"ISME J"},{"key":"1137_CR38","doi-asserted-by":"publisher","first-page":"e1002192","DOI":"10.1371\/journal.ppat.1002192","volume":"7","author":"M Kesarwani","year":"2011","unstructured":"Kesarwani M, Hazan R, He J, Que Y, Apidianakis Y, Lesic B, Xiao G, Dekimpe V, Milot S, Deziel E, et al.: A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes. PLoS Pathog. 2011, 7: e1002192-10.1371\/journal.ppat.1002192.","journal-title":"PLoS Pathog"},{"key":"1137_CR39","doi-asserted-by":"publisher","first-page":"6472","DOI":"10.1128\/JB.184.23.6472-6480.2002","volume":"184","author":"L Gallagher","year":"2002","unstructured":"Gallagher L, McKnight S, Kuznetsova M, Pesci E, Manoil C: Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa. J Bacteriol. 2002, 184: 6472-6480. 10.1128\/JB.184.23.6472-6480.2002.","journal-title":"J Bacteriol"},{"key":"1137_CR40","doi-asserted-by":"publisher","first-page":"7043","DOI":"10.1128\/JB.00753-08","volume":"190","author":"J Farrow III","year":"2008","unstructured":"Farrow III J, Sund Z, Ellison M, Wade D, Coleman J, Pesci E: PqsE functions independently of PqsR-Pseudomonas quinolone signal and enhances the rhl quorum-sensing system. J Bacteriol. 2008, 190: 7043-7051. 10.1128\/JB.00753-08.","journal-title":"J Bacteriol"},{"key":"1137_CR41","doi-asserted-by":"publisher","first-page":"16143","DOI":"10.1021\/ja3072397","volume":"134","author":"M Storz","year":"2012","unstructured":"Storz M, Maurer C, Zimmer C, Wagner N, Brengel C, de Jong J, Lucas S, M\u00fcsken M, H\u00e4ussler S, Steinbach A, Hartmann R: Validation of PqsD as anti-biofilm target in Pseudomonas aeruginosa by development of small molecule inhibitors. J Am Chem Soc. 2012, 134: 16143-16146. 10.1021\/ja3072397.","journal-title":"J Am Chem Soc"},{"key":"1137_CR42","doi-asserted-by":"publisher","first-page":"1496","DOI":"10.1021\/cb300208g","volume":"7","author":"T Klein","year":"2012","unstructured":"Klein T, Henn C, de Jong, Zimmer C, Kirsch B, Maurer C, Pistorius D, M\u00fcller R, Steinbach A, Hartmann R: Identification of small-molecule antagonists of the Pseudomonas aeruginosa transcriptional regulator PqsR: Biophysically guided hit discovery and optimization. ACS Chem Biol. 2012, 7: 1496-1501. 10.1021\/cb300208g.","journal-title":"ACS Chem Biol"},{"key":"1137_CR43","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.chembiol.2012.01.015","volume":"19","author":"C Lu","year":"2012","unstructured":"Lu C, Kirsch B, Zimmer C, de Jong J, Henn C, Maurer C, M\u00fcsken M, Steinbach A, Hartmann R: Discovery of antagonists of PqsR, a key player in 2-Alkyl-4-quinolone-dependent quorum sensing in Pseudomonas aeruginosa. Chem Biol. 2012, 19: 381-390. 10.1016\/j.chembiol.2012.01.015.","journal-title":"Chem Biol"},{"key":"1137_CR44","doi-asserted-by":"publisher","first-page":"10298","DOI":"10.1021\/bi900123j","volume":"48","author":"S Yu","year":"2009","unstructured":"Yu S, Jensen V, Seeliger J, Feldmann I, Weber S, Schleicher E, H\u00e4ussler S, Blankenfeldt W: Structure elucidation and preliminary assessment of hydrolase activity of PqsE, the Pseudomonas quinolone signal (PQS) response protein. Biochem. 2009, 48: 10298-10307. 10.1021\/bi900123j.","journal-title":"Biochem"},{"key":"1137_CR45","doi-asserted-by":"publisher","first-page":"6211","DOI":"10.1128\/JB.00888-09","volume":"191","author":"C Li","year":"2009","unstructured":"Li C, Wally H, Miller S, Lu C: The multifaceted proteins MvaT and MvaU, members of the H-NS family, control arginine metabolism, pyocyanin synthesis, and prophage activation in Pseudomonas aeruginosa PAO1. J Bacteriol. 2009, 191: 6211-6218. 10.1128\/JB.00888-09.","journal-title":"J Bacteriol"},{"key":"1137_CR46","doi-asserted-by":"publisher","first-page":"6454","DOI":"10.1128\/JB.183.21.6454-6465.2001","volume":"183","author":"D Mavrodi","year":"2001","unstructured":"Mavrodi D, Bonsall R, Delaney S, Soule M, Phillips G, Thomashow L: Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. J Bacteriol. 2001, 183: 6454-6465. 10.1128\/JB.183.21.6454-6465.2001.","journal-title":"J Bacteriol"}],"container-title":["BMC Systems Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1752-0509-7-81.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T23:10:27Z","timestamp":1715901027000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcsystbiol.biomedcentral.com\/articles\/10.1186\/1752-0509-7-81"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,21]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["1137"],"URL":"https:\/\/doi.org\/10.1186\/1752-0509-7-81","relation":{},"ISSN":["1752-0509"],"issn-type":[{"value":"1752-0509","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,21]]},"assertion":[{"value":"28 January 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 August 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 August 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"81"}}