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We first tested the feasibility of this method using <jats:italic>ADE2<\/jats:italic> as a marker and then analyzed some cell cycle regulators to reveal genetic interactions.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Using TIPI-gTOW, we successfully constructed a strain in which GFP-<jats:sup>TDegF<\/jats:sup>Ade2 was expressed at the lower limit, just sufficient to support cellular growth under the -Ade condition by accelerating degradation by TEV protease. We also succeeded in constructing a strain in which the minimal level of GFP-<jats:sup>TDegF<\/jats:sup>Cdc20 was expressed by TIPI-gTOW. Using this strain, we studied genetic interactions between cell cycle regulators and <jats:italic>CDC20<\/jats:italic>, and the result was highly consistent with the previously identified interactions. Comparison of the experimental data with predictions of a mathematical model revealed some interactions that were not implemented into the current model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>TIPI-gTOW is useful for estimating changes in the lower limit of a protein under different conditions, such as different genetic backgrounds and environments. TIPI-gTOW is also useful for analyzing genetic interactions of essential genes whose deletion mutants cannot be obtained.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1752-0509-8-2","type":"journal-article","created":{"date-parts":[[2014,1,7]],"date-time":"2014-01-07T02:01:15Z","timestamp":1389060075000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW"],"prefix":"10.1186","volume":"8","author":[{"given":"Masataka","family":"Sasabe","sequence":"first","affiliation":[]},{"given":"Sayumi","family":"Shintani","sequence":"additional","affiliation":[]},{"given":"Reiko","family":"Kintaka","sequence":"additional","affiliation":[]},{"given":"Kazunari","family":"Kaizu","sequence":"additional","affiliation":[]},{"given":"Koji","family":"makanae","sequence":"additional","affiliation":[]},{"given":"Hisao","family":"Moriya","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2014,1,7]]},"reference":[{"key":"1258_CR1","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1038\/ng1348","volume":"36","author":"A Zaslaver","year":"2004","unstructured":"Zaslaver A, Mayo AE, Rosenberg R, Bashkin P, Sberro H, Tsalyuk M, Surette MG, Alon U: Just-in-time transcription program in metabolic pathways. 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