{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T07:01:31Z","timestamp":1771657291159,"version":"3.50.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Diagnosis of amoebic liver abscess (ALA) in patients on anti-amoebic drugs is difficult. There is scanty data on this issue using <jats:italic>Entamoeba histolytica<\/jats:italic> (<jats:italic>E. histolytica<\/jats:italic>) lectin antigen and polymerase chain reaction (PCR). We studied utility of lectin antigen, PCR, and IgG antibody in diagnosis of liver abscess in patients on anti-amoebic treatment. Liver aspirate of 200 patients, of which 170 had anti-amoebic drug prior to drainage, was tested for <jats:italic>E. histolytica<\/jats:italic> lectin antigen by (ELISA), PCR, bacterial culture, and serum IgG antibody by (ELISA). Classification of abscesses was based on result of anti-amoebic IgG antibody and bacterial culture, <jats:italic>E. histolytica<\/jats:italic> PCR and bacterial culture, and <jats:italic>E. histolytica<\/jats:italic> lectin antigen and bacterial culture.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Using anti-amoebic IgG antibody and bacterial culture, 136\/200 (68.0%) were classified as ALA, 12\/200 (6.0%) as pyogenic liver abscess (PLA), 29\/200 (14.5%) as mixed infection, and 23\/200 (11.5%) remained unclassified. Using amoebic PCR and bacterial culture 151\/200 (75.5%) were classified as ALA, 25\/200 (12.5%) as PLA, 16\/200 (8.0%) as mixed infection, and 8\/200 (4.0%) remained unclassified. With <jats:italic>E. histolytica<\/jats:italic> lectin antigen and bacterial culture, 22\/200 (11.0%) patients were classified as ALA, 39\/200 (19.5%) as PLA, 2\/200 (1.0%) as mixed infection, and 137\/200 (68.5%) remained unclassified.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>\n              <jats:italic>E. histolytica<\/jats:italic> lectin antigen was not suitable for classification of ALA patients who had prior anti-amoebic treatment. However, PCR may be used as alternative test to anti-amoebic antibody in diagnosis of ALA.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-0500-5-416","type":"journal-article","created":{"date-parts":[[2012,8,7]],"date-time":"2012-08-07T10:14:11Z","timestamp":1344334451000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of antigen detection and polymerase chain reaction for diagnosis of amoebic liver abscess in patients on anti-amoebic treatment"],"prefix":"10.1186","volume":"5","author":[{"given":"Virendra","family":"Jaiswal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ujjala","family":"Ghoshal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay S","family":"Baijal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balraj","family":"Mittal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapan N","family":"Dhole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uday C","family":"Ghoshal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,8,7]]},"reference":[{"key":"1693_CR1","first-page":"497","volume":"52","author":"T Ahsan","year":"2002","unstructured":"Ahsan T, Jehangir MU, Mahmood T, Ahmed N, Saleem M, Shahid M, Shaheer A, Anwer A: Amoebic versus pyogenic liver abscess. 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