{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T17:52:42Z","timestamp":1784915562180,"version":"3.55.0"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Naltrexone has been proven to be an effective treatment option for the treatment of alcohol dependency. In this article we introduce a reliable and simple method developed for the simultaneous determination of naltrexone and 6-\u03b2-naltrexol in human serum by using high-performance liquid chromatography (HPLC).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Liquid-liquid extraction with butyl acetate from basic solutions (pH 9) was chosen for extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloric acid. The acid extract was chromatographed by HPLC with a reverse-phase ODS-column and electrochemical detector. The mobile phase was a NaH<jats:sub>2<\/jats:sub>PO<jats:sub>4<\/jats:sub>-solution with acetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium hydrogen sulphate as ion-pair reagents. The recovery of the extraction method was 48% for naltrexone and 75% for 6-\u03b2-naltrexol. The limit of quantification was 5.0\u2009ng\/ml for naltrexone and 1.0\u2009ng\/ml for 6-\u03b2-naltrexol. The analysed concentrations of naltrexone differed from the theoretic concentrations by 0.7 to 2.3% and those of 6-\u03b2-naltrexol by 2.6%. The relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-\u03b2-naltrexol; for the between-day assay it was 5.7% and 4.2%, respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our results indicate that the developed method is suitable for determination of naltrexone and 6-\u03b2-naltrexol in human serum.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-0500-5-439","type":"journal-article","created":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T18:15:16Z","timestamp":1345054516000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Analysis of naltrexone and its metabolite 6-beta-naltrexol in serum with high-performance liquid chromatography"],"prefix":"10.1186","volume":"5","author":[{"given":"Pekka","family":"Hein\u00e4l\u00e4","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuuli","family":"Lahti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sinclair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kari","family":"Ariniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pirjo","family":"Lillsunde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannu","family":"Alho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,8,15]]},"reference":[{"key":"1720_CR1","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1007\/s002130050686","volume":"139","author":"RZ Litten","year":"1998","unstructured":"Litten RZ, Allen JP: Advances in development of medications for alcoholism treatment. 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