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However, the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules do not permit transformation experiments with \u03b2-lactamase gene-containing vectors for certain human chlamydial pathogens. Therefore, a different selection marker is urgently needed for transformation of those chlamydiae.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>After transformation of plasmid-free <jats:italic>Chlamydia trachomatis<\/jats:italic> with pGFP:SW2, which carries a \u03b2-lactamase and a chloramphenicol acetyltransferase gene fused to a green fluorescence protein gene, transformants were obtained by selection with either ampicillin or chloramphenicol. Stable chloramphenicol-resistant, but ampicillin-sensitive, transformants were obtained using a pGFP:SW2 derivative without the \u03b2-lactamase. All transformants expressed green fluorescence protein and had glycogen synthesis activity restored.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Chloramphenicol resistance may be used as a selection marker for genetic experiments in <jats:italic>Chlamydia<\/jats:italic>. This eliminates the requirement for the use of \u03b2-lactamase, of which dissemination to some <jats:italic>C<\/jats:italic>. <jats:italic>trachomatis<\/jats:italic> serovars may jeopardize clinical treatment of chlamydial infections in pregnant women. Chloramphenicol acetyltransferase may also serve as a useful secondary selection marker for genetic analyses in \u03b2-lactamase-transformed chlamydial strains.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-0500-6-377","type":"journal-article","created":{"date-parts":[[2013,9,24]],"date-time":"2013-09-24T00:27:53Z","timestamp":1379982473000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Chloramphenicol acetyltransferase as a selection marker for chlamydial transformation"],"prefix":"10.1186","volume":"6","author":[{"given":"Shuang","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Battaglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhou","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,9,23]]},"reference":[{"key":"2406_CR1","first-page":"139","volume-title":"Chlamydia Intracellular Biology, Pathogenesis","author":"J Schachter","year":"1999","unstructured":"Schachter J: Infection and disease epidemiology. 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