{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T22:23:08Z","timestamp":1743891788886},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Extended spectrum \u00df-lactamases (ESBLs) represent a major group of lactamases responsible for resistance, mostly produced by gram-negative bacteria, to newer generations of \u00df-lactam drugs currently being identified in large numbers worldwide. The present study was undertaken to see the frequency of ESBL producing <jats:italic>Pseudomonas<\/jats:italic> spp. isolated from six hundred clinical specimens (wound, pus, aural, urine, sputum, throat and other swabs) collected over a period of three years from two tertiary care hospitals in Bangladesh.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Aerobic bacterial culture was performed on aseptically collected swabs and only growth of <jats:italic>Pseudomonas<\/jats:italic> was considered for further species identification and ESBL production along with serotyping of <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer agar diffusion method and ESBL production was detected on Mueller Hinton agar by double-disk synergy technique using Amoxicillin-Clavulanic acid with Ceftazidime, Cefotaxime, Ceftriaxone and Aztreonam. Culture yielded 120 <jats:italic>Pseudomonas<\/jats:italic> spp<jats:italic>.<\/jats:italic> and 82 of them were biochemically characterized for species. <jats:italic>Pseudomonas aeruginosa<\/jats:italic> was found to be the predominant (90.2%) species. Of 82 isolates tested for ESBL, 31 (37.8%) were ESBL positive with 29 (93.5%) as <jats:italic>Pseudomonas aeruginosa,<\/jats:italic> the remaining 2 (6.5%) were <jats:italic>Stenotrophomonas maltophilia<\/jats:italic> and <jats:italic>Ralstonia pickettii.<\/jats:italic> Antibiogram revealed Imipenem as the most effective drug (93.3%) among all antimicrobials used against <jats:italic>Pseudomonas<\/jats:italic> spp. followed by Aminoglycosides (63.7%).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>ESBL producing <jats:italic>Pseudomonas<\/jats:italic> spp. was found to be a frequent isolate from two tertiary care hospitals in Bangladesh, showing limited susceptibility to antimicrobials and decreased susceptibility to Imipenem in particular, which is a matter of great concern.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-0500-6-7","type":"journal-article","created":{"date-parts":[[2013,1,5]],"date-time":"2013-01-05T15:14:10Z","timestamp":1357398850000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Detection of extended spectrum \u03b2-lactamase in Pseudomonas spp. isolated from two tertiary care hospitals in Bangladesh"],"prefix":"10.1186","volume":"6","author":[{"given":"Shahanara","family":"Begum","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Abdus","family":"Salam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kh Faisal","family":"Alam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurjahan","family":"Begum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pervez","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalaluddin Ashraful","family":"Haq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,1,5]]},"reference":[{"key":"1974_CR1","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3349\/ymj.2010.51.1.111","volume":"51","author":"D Dundar","year":"2010","unstructured":"Dundar D, Otkun M: In-vitro efficacy of synergistic antibiotic combinations in multidrug resistant pseudomonas aeruginosa strains. 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