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Current phenotypic techniques fail to reflect the full extent of the diversity of<jats:italic>Fasciola<\/jats:italic>spp. In this respect, the use of molecular techniques to identify and differentiate<jats:italic>Fasciola<\/jats:italic>spp. offer considerable advantages. The advent of a variety of molecular genetic techniques also provides a powerful method to elucidate many aspects of<jats:italic>Fasciola<\/jats:italic>biology, epidemiology, and genetics. However, the discriminatory power of these molecular methods varies, as does the speed and ease of performance and cost. There is a need for the development of new methods to identify the mechanisms underpinning the origin and maintenance of genetic variation within and among<jats:italic>Fasciola<\/jats:italic>populations. The increasing application of the current and new methods will yield a much improved understanding of<jats:italic>Fasciola<\/jats:italic>epidemiology and evolution as well as more effective means of parasite control. Herein, we provide an overview of the molecular techniques that are being used for the genetic characterization, detection and genotyping of<jats:italic>Fasciola<\/jats:italic>spp..<\/jats:p>","DOI":"10.1186\/1756-3305-4-101","type":"journal-article","created":{"date-parts":[[2011,6,10]],"date-time":"2011-06-10T10:03:11Z","timestamp":1307700191000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches"],"prefix":"10.1186","volume":"4","author":[{"given":"Lin","family":"Ai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu-Xin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samer","family":"Alasaad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany M","family":"Elsheikha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Long","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-Qing","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Cai","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Xu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,6,10]]},"reference":[{"key":"337_CR1","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1016\/j.ijpara.2005.07.010","volume":"35","author":"S Mas-Coma","year":"2005","unstructured":"Mas-Coma S, Bargues MD, Valero MA: Fascioliasis and other plant-borne trematode zoonoses. 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