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The purpose of this study was to identify a cathepsin B of <jats:italic>Clonorchis sinensis<\/jats:italic> (<jats:italic>Cs<\/jats:italic> CB) and to investigate its diagnostic value for human helminthiases.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The predicted amino acid sequence of the cathepsin B of <jats:italic>C. sinensis<\/jats:italic> shared 63%, 52%, 50% identity with that of <jats:italic>Schistosoma japonicum<\/jats:italic>, <jats:italic>Homo sapiens<\/jats:italic> and <jats:italic>Fasciola hepatica<\/jats:italic>, respectively. Sequence encoding proenzyme of <jats:italic>Cs<\/jats:italic> CB was overexpressed in <jats:italic>Escherichia coli<\/jats:italic>. Reverse transcription PCR experiments revealed that <jats:italic>Cs<\/jats:italic> CB transcribed in both adult worm and metacercaria of <jats:italic>C. sinensis<\/jats:italic>. <jats:italic>Cs<\/jats:italic> CB was identified as a <jats:italic>C. sinensis<\/jats:italic> excretory\/secretory product by immunoblot assay, which was consistent with immunohistochemical localization showing that <jats:italic>Cs<\/jats:italic> CB was especially expressed in the intestine of <jats:italic>C. sinensis<\/jats:italic> adults. Both ELISA and western blotting analysis showed recombinant <jats:italic>Cs<\/jats:italic> CB could react with human sera from clonorchiasis and other helminthiases.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our findings revealed that secreted CsCB may play an important role in the biology of C. sinensis and could be a diagnostic candidate for helminthiases.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-4-149","type":"journal-article","created":{"date-parts":[[2011,7,27]],"date-time":"2011-07-27T18:22:26Z","timestamp":1311790946000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Molecular characterization of cathepsin B from Clonorchis sinensis excretory\/secretory products and assessment of its potential for serodiagnosis of clonorchiasis"],"prefix":"10.1186","volume":"4","author":[{"given":"Wenjun","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuerong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenhui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanhuan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huali","family":"Lei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingtao","family":"Men","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongxiu","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinbing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,7,27]]},"reference":[{"key":"397_CR1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S1473-3099(04)01252-6","volume":"5","author":"ZR Lun","year":"2005","unstructured":"Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, Fang YY: Clonorchiasis: a key foodborne zoonosis in China. 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