{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:33:20Z","timestamp":1790393600974,"version":"4.1.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>This research was carried out to develop a reliable monoclonal antibody (MoAb)-based sandwich enzyme linked immunosorbent assay (ELISA) for the diagnosis of active <jats:italic>Fasciola gigantica<\/jats:italic> infection in both serum and stool for comparative purposes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>From a panel of MoAbs raised against <jats:italic>F. gigantica<\/jats:italic> excretory\/secretory antigens (ES Ags), a pair (12B\/11D\/3F and 10A\/9D\/10G) was chosen due to its high reactivity and strict specificity to <jats:italic>F. gigantica<\/jats:italic> antigen by indirect ELISA.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The two MoAbs were of the IgG<jats:sub>1<\/jats:sub> and IgG<jats:sub>2a<\/jats:sub> subclasses, respectively. Using SDS-PAGE and EITB, the selected MoAbs recognized 83, 64, 45 and 26 kDa bands of ES Ags. The lower detection limit of ELISA assay was 3 ng\/ml. In stool, the sensitivity, specificity and diagnostic efficacy of ELISA was 96%, 98.2 and 97.1%; while in serum they were 94%, 94.6% and 94.3%, respectively. Moreover, a positive correlation was found between ova count in stool of <jats:italic>F. gigantica<\/jats:italic> infected patients and the OD readings of ELISA in both stool and serum samples (<jats:italic>r<\/jats:italic> = 0.730, p &lt; 0.01 and r = 0.608; p &lt; 0.01, respectively).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These data showed that the use of MoAb-based sandwich ELISA for the detection of <jats:italic>F. gigantica<\/jats:italic> coproantigens in stool specimens was superior to serum samples; it provides a highly efficient, non-invasive technique for the diagnosis of active <jats:italic>F. gigantica<\/jats:italic> infection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-4-176","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T10:37:03Z","timestamp":1316083023000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory\/secretory antigens in both serum and stool"],"prefix":"10.1186","volume":"4","author":[{"given":"Zeinab A","family":"Demerdash","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek M","family":"Diab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim R","family":"Aly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salwa H","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faten S","family":"Mahmoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona K","family":"Zoheiry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafaa A","family":"Mansour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohy E","family":"Attia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azza E","family":"El-Bassiouny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,9,15]]},"reference":[{"key":"422_CR1","first-page":"113","volume-title":"Fasciolosis","author":"P Torgerson","year":"1999","unstructured":"Torgerson P, Claxton J: Epidemiology and control. 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