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Early identification of the infection is essential to provide timely and appropriate chemotherapy to patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study we evaluate a PCR-based molecular identification method, Multiplex Ligation-dependent Probe Amplification (MLPA), which allows rapid and specific detection of single nucleotide acid differences between <jats:italic>Clonorchis sinensis<\/jats:italic>, <jats:italic>Opisthorchis viverrini<\/jats:italic> and <jats:italic>O. felineus<\/jats:italic>. Three probe pairs were derived from the Internally Transcribed Spacer 1 (ITS1) of three opisthorchid liver flukes using a systematic phylogenetic analysis. Specific loci were detected in all three species, yielding three amplicons with 198,172 and 152 bp, respectively, while no cross reactions were observed. A panel of 66 <jats:italic>C. sinensis<\/jats:italic> isolates was screened using MLPA. All species were positively identified, and no inhibition was observed. The detection limit was 10<jats:sup>3<\/jats:sup> copies of the ITS gene for the three liver flukes, or about 60 pg genomic DNA for <jats:italic>Clonorchis sinensis<\/jats:italic>. Amplification products can be detected by electrophoresis on agarose gel or in a capillary sequencer. In addition, genomic DNA of <jats:italic>Clonorchis sinensis<\/jats:italic> in fecal samples of infected rats was positively amplified by MLPA.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The flexibility and specificity make MLPA a potential tool for specific identification of infections by opisthorchid liver flukes in endemic areas.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-4-98","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T18:31:23Z","timestamp":1307471483000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)"],"prefix":"10.1186","volume":"4","author":[{"given":"Jiufeng","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhuan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G S","family":"de Hoog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinbing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,6,7]]},"reference":[{"key":"339_CR1","first-page":"444","volume-title":"Fisheries technical paper","author":"FAO","year":"2004","unstructured":"FAO: Assessment and management of seafood quality and safety. 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