{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T11:53:35Z","timestamp":1793620415817,"version":"4.5.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The human parasite <jats:italic>Onchocerca volvulus<\/jats:italic> harbours <jats:italic>Wolbachia<\/jats:italic> endosymbionts essential for worm embryogenesis, larval development and adult survival. In this study, the development of <jats:italic>Wolbachia<\/jats:italic>-depleted microfilariae (first stage larvae) to infective third stage larvae (L3) in the insect vector <jats:italic>Simulium damnosum<\/jats:italic> was analysed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Infected volunteers in Cameroon were randomly and blindly allocated into doxycycline (200 mg\/day for 6 weeks) or placebo treatment groups. After treatment, blackflies were allowed to take a blood meal on the volunteers, captured and dissected for larval counting and DNA extraction for quantitative real-time PCR analysis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>PCR results showed a clear reduction in <jats:italic>Wolbachia<\/jats:italic> DNA after doxycycline treatment in microfilariae from human skin biopsies with &gt; 50% reduction at one month post-treatment, eventually reaching a reduction of &gt; 80%. Larval stages recovered from the insect vector had similar levels of reduction of endosymbiotic bacteria. Larval recoveries were analysed longitudinally after treatment to follow the kinetics of larval development. Beginning at three months post-treatment, significantly fewer L3 were seen in the blackflies that had fed on doxycycline treated volunteers. Concomitant with this, the proportion of second stage larvae (L2) was significantly increased in this group.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Doxycycline treatment and the resulting decline of <jats:italic>Wolbachia<\/jats:italic> endobacteria from the microfilaria resulted in retarded development of larvae in the insect vector. Thus, anti-wolbachial treatment could have an additive effect for interrupting transmission by reducing the number of L3 that can be transmitted by blackflies.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-5-12","type":"journal-article","created":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T19:24:04Z","timestamp":1326309844000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Retarded Onchocerca volvulus L1 to L3 larval development in the Simulium damnosum vector after anti-wolbachial treatment of the human host"],"prefix":"10.1186","volume":"5","author":[{"given":"Anna","family":"Albers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias Eyong","family":"Esum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Tendongfor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Enyong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Klarmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Wanji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Hoerauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Pfarr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,1,11]]},"reference":[{"key":"532_CR1","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S0065-308X(05)61009-3","volume":"61","author":"BA Boatin","year":"2006","unstructured":"Boatin BA, Richards FO: Control of onchocerciasis. 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