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The Lulo cell line, derived from<jats:italic>Lutzomyia longipalpis<\/jats:italic>(Diptera: Psychodidae), is a suitable<jats:italic>in vitro<\/jats:italic>study model to understand the features of parasite adhesion<jats:italic>.<\/jats:italic>Here, we analyze the role of glycosaminoglycans (GAGs) from Lulo cells and proteins from the parasites in this event.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Flagellar (F<jats:sub><jats:italic>f<\/jats:italic><\/jats:sub>) and membrane (M<jats:sub><jats:italic>f<\/jats:italic><\/jats:sub>) fractions from promastigotes were obtained by differential centrifugation and the purity of fractions confirmed by western blot assays, using specific antibodies for cellular compartments. Heparin-binding proteins (HBP) were isolated from both fractions using a HiTrap-Heparin column. In addition, binding of promastigotes to Lulo cells or to a heparin-coated surface was assessed by inhibition assays or surface plasmon resonance (SPR) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The success of promastigotes subcellular fractionation led to the obtainment of F<jats:sub>f<\/jats:sub>and M<jats:sub>f<\/jats:sub>proteins, both of which presented two main protein bands (65.0 and 55.0kDa) with affinity to heparin. The contribution of HBPs in the adherence of promastigotes to Lulo cells was assessed through competition assays, using HS or the purified HBPs fractions. All tested samples presented a measurable inhibition rate when compared to control adhesion rate (17\u2009\u00b1\u20092.0% of culture cells with adhered parasites): 30% (for HS 20\u03bcg\/ml) and 16% (for HS 10\u03bcg\/ml); HBP M<jats:sub>f<\/jats:sub>(35.2% for 10\u03bcg\/ml and 25.4% for 20\u03bcg\/ml) and HBP F<jats:sub>f<\/jats:sub>(10.0% for 10\u03bcg\/ml and 31.4% for 20\u03bcg\/ml). Additionally, to verify the presence of sulfated GAGs in Lulo cells surface and intracellular compartment, metabolic labeling with radioactive sulfate was performed, indicating the presence of an HS and chondroitin sulfate in both cell sections. The SPR analysis performed further confirmed the presence of GAGs ligands on<jats:italic>L. (V.) braziliensis<\/jats:italic>promastigote surfaces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The data presented here point to evidences that HBPs present on the surface of<jats:italic>L. (V.) braziliensis<\/jats:italic>promastigotes participate in adhesion of these parasites to Lulo cells through HS participation.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1756-3305-5-142","type":"journal-article","created":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T15:49:47Z","timestamp":1342540187000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitro"],"prefix":"10.1186","volume":"5","author":[{"given":"Luzia Monteiro","family":"de Castro C\u00f4rtes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirian Claudia","family":"de Souza Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin Souza","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo Ac\u00e1cio Santini","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Od\u00eancio","family":"de Oliveira Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Oliveira","family":"de Ara\u00fajo Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reginaldo Pe\u00e7anha","family":"Brazil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leny","family":"Toma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Meloni","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena Bonciani","family":"Nader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"de F\u00e1tima Madeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felio J","family":"Bello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Roberto","family":"Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,7,17]]},"reference":[{"key":"610_CR1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1016\/S1473-3099(07)70209-8","volume":"7","author":"R Reithinger","year":"2007","unstructured":"Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, Brooker S: Cutaneous leishmaniasis. 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