{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T20:14:35Z","timestamp":1693080875214},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>New interventions are required to optimally and sustainably control the <jats:italic>Anopheles<\/jats:italic> sp. mosquitoes that transmit malaria and filariasis. The mosquito olfactory system is important in host seeking (transmission) and mate finding (reproduction). Understanding olfactory function could lead to development of control strategies based on repelling parasite-carrying mosquitoes or attracting them into a fatal trap.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Our initial focus is on odorant binding proteins with differential transcript accumulation between female and male mosquitoes. We report that the odorant binding protein, OBP2 (AGAP003306), had increased expression in the antennae of female vs. male <jats:italic>Anopheles gambiae<\/jats:italic>\n              <jats:italic>sensu stricto<\/jats:italic> (G3 strain). The increased expression in antennae of females of this gene by quantitative RT-PCR was 4.2 to 32.3 fold in three independent biological replicates and two technical replicate experiments using <jats:italic>A. gambiae<\/jats:italic> from two different laboratories. OBP2 is a member of the vast OBP superfamily of insect odorant binding proteins and belongs to the predominantly dipteran clade that includes the <jats:italic>Culex<\/jats:italic> oviposition kairomone-binding OBP1. Phylogenetic analysis indicates that its orthologs are present across culicid mosquitoes and are likely to play a conserved role in recognizing a molecule that might be critical for female behavior.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>OBP2 has increased mRNA transcript accumulation in the antennae of female as compared to male <jats:italic>A. gambiae<\/jats:italic>. This molecule and related molecules may play an important role in female mosquito feeding and breeding behavior. This finding may be a step toward providing a foundation for understanding mosquito olfactory requirements and developing control strategies based on reducing mosquito feeding and breeding success.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-5-27","type":"journal-article","created":{"date-parts":[[2012,2,7]],"date-time":"2012-02-07T15:14:36Z","timestamp":1328627676000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Female Anopheles gambiae antennae: increased transcript accumulation of the mosquito-specific odorant-binding-protein OBP2"],"prefix":"10.1186","volume":"5","author":[{"given":"Seth A","family":"Hoffman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshminarayanan","family":"Aravind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soundarapandian","family":"Velmurugan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,2,6]]},"reference":[{"key":"519_CR1","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1016\/j.ibmb.2004.03.017","volume":"34","author":"LJ Zwiebel","year":"2004","unstructured":"Zwiebel LJ, Takken W: Olfactory regulation of mosquito-host interactions. Insect Biochem Mol Biol. 2004, 34: 645-652. 10.1016\/j.ibmb.2004.03.017.","journal-title":"Insect Biochem Mol Biol"},{"key":"519_CR2","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1007\/s00359-005-0044-y","volume":"191","author":"M Rutzler","year":"2005","unstructured":"Rutzler M, Zwiebel LJ: Molecular biology of insect olfaction: recent progress and conceptual models. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2005, 191: 777-790. 10.1007\/s00359-005-0044-y.","journal-title":"J Comp Physiol A Neuroethol Sens Neural Behav Physiol"},{"key":"519_CR3","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S0065-2660(08)00802-X","volume":"64","author":"XG Chen","year":"2008","unstructured":"Chen XG, Mathur G, James AA: Gene expression studies in mosquitoes. Adv Genet. 2008, 64: 19-50.","journal-title":"Adv Genet"},{"key":"519_CR4","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/1756-3305-4-10","volume":"4","author":"E Maekawa","year":"2011","unstructured":"Maekawa E, Aonuma H, Nelson B, Yoshimura A, Tokunaga F, Fukumoto S, Kanuka H: The role of proboscis of the malaria vector mosquito Anopheles stephensi in host-seeking behavior. Parasit Vectors. 2011, 4: 10-10.1186\/1756-3305-4-10.","journal-title":"Parasit Vectors"},{"key":"519_CR5","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1038\/293161a0","volume":"293","author":"RG Vogt","year":"1981","unstructured":"Vogt RG, Riddiford LM: Pheromone binding and inactivation by moth antennae. Nature. 1981, 293: 161-163. 10.1038\/293161a0.","journal-title":"Nature"},{"key":"519_CR6","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.1007\/s00018-005-5607-0","volume":"63","author":"P Pelosi","year":"2006","unstructured":"Pelosi P, Zhou JJ, Ban LP, Calvello M: Soluble proteins in insect chemical communication. Cell Mol Life Sci. 2006, 63: 1658-1676. 10.1007\/s00018-005-5607-0.","journal-title":"Cell Mol Life Sci"},{"key":"519_CR7","doi-asserted-by":"publisher","first-page":"e9471","DOI":"10.1371\/journal.pone.0009471","volume":"5","author":"H Biessmann","year":"2010","unstructured":"Biessmann H, Andronopoulou E, Biessmann MR, Douris V, Dimitratos SD, Eliopoulos E, Guerin PM, Iatrou K, Justice RW, Krober T: The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes. PLoS One. 2010, 5: e9471-10.1371\/journal.pone.0009471.","journal-title":"PLoS One"},{"key":"519_CR8","doi-asserted-by":"publisher","first-page":"5058","DOI":"10.1073\/pnas.0308146101","volume":"101","author":"RJ Pitts","year":"2004","unstructured":"Pitts RJ, Fox AN, Zwiebel LJ: A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae. Proc Natl Acad Sci USA. 2004, 101: 5058-5063. 10.1073\/pnas.0308146101.","journal-title":"Proc Natl Acad Sci USA"},{"key":"519_CR9","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/j.1365-2583.2005.00590.x","volume":"14","author":"H Biessmann","year":"2005","unstructured":"Biessmann H, Nguyen QK, Le D, Walter MF: Microarray-based survey of a subset of putative olfactory genes in the mosquito Anopheles gambiae. Insect Mol Biol. 2005, 14: 575-589. 10.1111\/j.1365-2583.2005.00590.x.","journal-title":"Insect Mol Biol"},{"key":"519_CR10","doi-asserted-by":"publisher","first-page":"e15403","DOI":"10.1371\/journal.pone.0015403","volume":"5","author":"W Xu","year":"2010","unstructured":"Xu W, Cornel AJ, Leal WS: Odorant-binding proteins of the malaria mosquito Anopheles funestus sensu stricto. PLoS One. 2010, 5: e15403-10.1371\/journal.pone.0015403.","journal-title":"PLoS One"},{"key":"519_CR11","doi-asserted-by":"publisher","first-page":"614","DOI":"10.7150\/ijbs.6.614","volume":"6","author":"D Schymura","year":"2010","unstructured":"Schymura D, Forstner M, Schultze A, Krober T, Swevers L, Iatrou K, Krieger J: Antennal expression pattern of two olfactory receptors and an odorant binding protein implicated in host odor detection by the malaria vector Anopheles gambiae. Int J Biol Sci. 2010, 6: 614-626.","journal-title":"Int J Biol Sci"},{"key":"519_CR12","doi-asserted-by":"publisher","first-page":"e1000681","DOI":"10.1371\/journal.pgen.1000681","volume":"5","author":"S Zhou","year":"2009","unstructured":"Zhou S, Stone EA, Mackay TF, Anholt RR: Plasticity of the chemoreceptor repertoire in Drosophila melanogaster. PLoS Genet. 2009, 5: e1000681-10.1371\/journal.pgen.1000681.","journal-title":"PLoS Genet"},{"key":"519_CR13","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1186\/1471-2164-12-271","volume":"12","author":"RJ Pitts","year":"2011","unstructured":"Pitts RJ, Rinker DC, Jones PL, Rokas A, Zwiebel LJ: Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding. BMC Genomics. 2011, 12: 271-10.1186\/1471-2164-12-271.","journal-title":"BMC Genomics"},{"key":"519_CR14","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/1756-3305-4-109","volume":"4","author":"YE Himeidan","year":"2011","unstructured":"Himeidan YE, Elzaki MM, Kweka EJ, Ibrahim M, Elhassan IM: Pattern of malaria transmission along the Rahad River basin, Eastern Sudan. Parasit Vectors. 2011, 4: 109-10.1186\/1756-3305-4-109.","journal-title":"Parasit Vectors"},{"key":"519_CR15","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1016\/S0035-9203(96)90414-9","volume":"90","author":"SK Dunyo","year":"1996","unstructured":"Dunyo SK, Appawu M, Nkrumah FK, Baffoe-Wilmot A, Pedersen EM, Simonsen PE: Lymphatic filariasis on the coast of Ghana. Tran R Soc Trop Med Hyg. 1996, 90: 634-638. 10.1016\/S0035-9203(96)90414-9.","journal-title":"Tran R Soc Trop Med Hyg"},{"key":"519_CR16","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1006\/viro.1998.9437","volume":"252","author":"RS Lanciotti","year":"1998","unstructured":"Lanciotti RS, Ludwig ML, Rwaguma EB, Lutwama JJ, Kram TM, Karabatsos N, Cropp BC, Miller BR: Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virus. Virology. 1998, 252: 258-268. 10.1006\/viro.1998.9437.","journal-title":"Virology"},{"key":"519_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1365-2583.2006.00610.x","volume":"15","author":"O Marinotti","year":"2006","unstructured":"Marinotti O, Calvo E, Nguyen QK, Dissanayake S, Ribeiro JM, James AA: Genome-wide analysis of gene expression in adult Anopheles gambiae. Insect Mol Biol. 2006, 15: 1-12. 10.1111\/j.1365-2583.2006.00610.x.","journal-title":"Insect Mol Biol"},{"key":"519_CR18","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-94-009-1535-0_1","volume-title":"The Molecular Biology of Insect Disease Vectors: A Methods Manual","author":"MQ Benedict","year":"1997","unstructured":"Benedict MQ: Care and maintenance of anopheline mosquito colonies. The Molecular Biology of Insect Disease Vectors: A Methods Manual. Edited by: Crampton JM, Beard CB, Louis C. 1997, London: Chapman and Hall, 1: 3-12."},{"key":"519_CR19","doi-asserted-by":"publisher","first-page":"4147","DOI":"10.1093\/nar\/21.17.4147","volume":"21","author":"CE Salazar","year":"1993","unstructured":"Salazar CE, Mills-Hamm D, Kumar V, Collins FH: Sequence of a cDNA from the mosquito Anopheles gambiae encoding a homologue of human ribosomal protein S7. Nucleic Acids Res. 1993, 21: 4147-10.1093\/nar\/21.17.4147.","journal-title":"Nucleic Acids Res"},{"key":"519_CR20","first-page":"365","volume":"132","author":"S Rozen","year":"2000","unstructured":"Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.","journal-title":"Methods Mol Biol"},{"key":"519_CR21","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001, 25: 402-408. 10.1006\/meth.2001.1262.","journal-title":"Methods"},{"key":"519_CR22","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008, 3: 1101-1108. 10.1038\/nprot.2008.73.","journal-title":"Nat Protoc"},{"key":"519_CR23","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1093\/nar\/gkn1006","volume":"37","author":"T Lassmann","year":"2009","unstructured":"Lassmann T, Frings O, Sonnhammer EL: Kalign2: high-performance multiple alignment of protein and nucleotide sequences allowing external features. Nucleic Acids Res. 2009, 37: 858-865. 10.1093\/nar\/gkn1006.","journal-title":"Nucleic Acids Res"},{"key":"519_CR24","doi-asserted-by":"publisher","first-page":"e9490","DOI":"10.1371\/journal.pone.0009490","volume":"5","author":"MN Price","year":"2010","unstructured":"Price MN, Dehal PS, Arkin AP: FastTree 2--approximately maximum-likelihood trees for large alignments. PLoS One. 2010, 5: e9490-10.1371\/journal.pone.0009490.","journal-title":"PLoS One"},{"key":"519_CR25","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1101\/gr.174302","volume":"12","author":"O Lespinet","year":"2002","unstructured":"Lespinet O, Wolf YI, Koonin EV, Aravind L: The role of lineage-specific gene family expansion in the evolution of eukaryotes. Genome Res. 2002, 12: 1048-1059. 10.1101\/gr.174302.","journal-title":"Genome Res"},{"key":"519_CR26","doi-asserted-by":"publisher","first-page":"19102","DOI":"10.1073\/pnas.1012274107","volume":"107","author":"Y Mao","year":"2010","unstructured":"Mao Y, Xu X, Xu W, Ishida Y, Leal WS, Ames JB, Clardy J: Crystal and solution structures of an odorant-binding protein from the southern house mosquito complexed with an oviposition pheromone. Proc Natl Acad Sci USA. 2010, 107: 19102-19107. 10.1073\/pnas.1012274107.","journal-title":"Proc Natl Acad Sci USA"},{"key":"519_CR27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4269\/ajtmh.2004.71.2_suppl.0700001","volume":"71","author":"JG Breman","year":"2004","unstructured":"Breman JG, Alilio MS, Mills A: Conquering the intolerable burden of malaria: what's new, what's needed: a summary. Am J Trop Med Hyg. 2004, 71: 1-15.","journal-title":"Am J Trop Med Hyg"},{"key":"519_CR28","volume-title":"WHO","author":"WHO","year":"2008","unstructured":"WHO: The global malaria action plan for a malaria free world. Geneva: Roll Back Malaria Partnership (RBMP). WHO. 2008, 274:"},{"key":"519_CR29","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1126\/science.1076181","volume":"298","author":"RA Holt","year":"2002","unstructured":"Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R: The genome sequence of the malaria mosquito Anopheles gambiae. Science. 2002, 298: 129-149. 10.1126\/science.1076181.","journal-title":"Science"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1756-3305-5-27.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T18:31:16Z","timestamp":1630521076000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/1756-3305-5-27"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,6]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["519"],"URL":"https:\/\/doi.org\/10.1186\/1756-3305-5-27","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,6]]},"assertion":[{"value":"12 January 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"27"}}