{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:51:26Z","timestamp":1792702286303,"version":"4.5.0"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Ehrlichia<\/jats:italic> species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious and fatal infections in companion animals and livestock. The aim of this paper was to phylogeneticaly characterise a new species of <jats:italic>Ehrlichia<\/jats:italic> isolated from <jats:italic>Rhipicephalus (Boophilus) microplus<\/jats:italic> from Minas Gerais, Brazil.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The agent was isolated from the hemolymph of <jats:italic>Rhipicephalus (B.) microplus<\/jats:italic> engorged females that had been collected from naturally infested cattle in a farm in the state of Minas Gerais, Brazil. This agent was then established and cultured in IDE8 tick cells. The molecular and phylogenetic analysis was based on <jats:italic>16S rRNA<\/jats:italic>, <jats:italic>groEL, dsb<\/jats:italic>, <jats:italic>gltA<\/jats:italic> and <jats:italic>gp36<\/jats:italic> genes. We used the maximum likelihood method to construct the phylogenetic trees.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The phylogenetic trees based on <jats:italic>16S rRNA<\/jats:italic>, <jats:italic>groEL, dsb<\/jats:italic> and <jats:italic>gltA<\/jats:italic> showed that the <jats:italic>Ehrlichia<\/jats:italic> spp isolated in this study falls in a clade separated from any previously reported <jats:italic>Ehrlichia<\/jats:italic> spp. The molecular analysis of the ortholog of <jats:italic>gp36,<\/jats:italic> the major immunoreactive glycoproteins in <jats:italic>E. canis<\/jats:italic> and ortholog of the <jats:italic>E. chaffeensis gp47,<\/jats:italic> showed a unique tandem repeat of 9 amino acids (VPAASGDAQ) when compared with those reported for <jats:italic>E. canis<\/jats:italic>, <jats:italic>E. chaffeensis<\/jats:italic> and the related mucin-like protein in <jats:italic>E. ruminantium<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Based on the molecular and phylogenetic analysis of the <jats:italic>16S rRNA<\/jats:italic>, <jats:italic>groEL, dsb<\/jats:italic> and <jats:italic>gltA<\/jats:italic> genes we concluded that this tick-derived microorganism isolated in Brazil is a new species, named <jats:italic>E. mineirensis<\/jats:italic> (UFMG-EV), with predicted novel antigenic properties in the <jats:italic>gp36<\/jats:italic> ortholog glycoprotein. Further studies on this new <jats:italic>Ehrlichia<\/jats:italic> spp should address questions about its transmissibility by ticks and its pathogenicity for mammalian hosts.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-5-291","type":"journal-article","created":{"date-parts":[[2012,12,11]],"date-time":"2012-12-11T13:14:27Z","timestamp":1355231667000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats"],"prefix":"10.1186","volume":"5","author":[{"given":"Alejandro Cabezas","family":"Cruz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich","family":"Zweygarth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mucio Flavio Barbosa","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Angelica Gon\u00e7alves","family":"da Silveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libor","family":"Grubhoffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J","family":"Vald\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lygia Maria Friche","family":"Passos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,12,11]]},"reference":[{"issue":"4","key":"770_CR1","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/S1525-1578(10)60581-8","volume":"7","author":"KC Doyle","year":"2005","unstructured":"Doyle KC, Labruna MB, Breitschwerdt EB, Tang YW, Corstvet RE, Hegarty BC, Bloch KC, Li P, Walker DH, McBride JW: Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene. 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