{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T22:26:59Z","timestamp":1776292019455,"version":"3.50.1"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>The lack of genomic data available for mites limits our understanding of their biology. Evolving high-throughput sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome size are initially required to ensure sufficient coverage.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Quantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite <jats:italic>Sarcoptes scabiei<\/jats:italic>, the non-burrowing ectoparasitic mite <jats:italic>Psoroptes ovis<\/jats:italic>, and the free-living house dust mite <jats:italic>Dermatophagoides pteronyssinus<\/jats:italic>. Additionally, the chromosome number of <jats:italic>S. scabiei<\/jats:italic> was determined by chromosomal spreads of embryonic cells derived from single eggs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>\n              <jats:italic>S. scabiei<\/jats:italic> cells were shown to contain 17 or 18 small (&lt; 2 \u03bcM) chromosomes, suggesting an XO sex-determination mechanism. The average estimated genome sizes of <jats:italic>S. scabiei<\/jats:italic> and <jats:italic>P. ovis<\/jats:italic> were 96 (\u00b1 7) Mb and 86 (\u00b1 2) Mb respectively, among the smallest arthropod genomes reported to date. The <jats:italic>D. pteronyssinus<\/jats:italic> genome was estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This data provides a starting point for understanding the genetic organisation and evolution of these astigmatid mites, informing future sequencing projects. A comparitive genomic approach including these three closely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-5-3","type":"journal-article","created":{"date-parts":[[2012,1,4]],"date-time":"2012-01-04T12:50:39Z","timestamp":1325681439000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus"],"prefix":"10.1186","volume":"5","author":[{"given":"Kate E","family":"Mounsey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlene","family":"Willis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stewart TG","family":"Burgess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah C","family":"Holt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"McCarthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,1,4]]},"reference":[{"key":"492_CR1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.4269\/ajtmh.2003.68.1.0680061","volume":"68","author":"K Fischer","year":"2003","unstructured":"Fischer K, Holt DC, Harumal P, Currie BJ, Walton SF, Kemp DJ: Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens. 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