{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T13:57:49Z","timestamp":1794491869452,"version":"4.9.1"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T00:00:00Z","timestamp":1373846400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1186\/1756-3305-6-207","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T06:15:55Z","timestamp":1373868955000},"source":"Crossref","is-referenced-by-count":8,"title":["Luminescent multiplex viability assay for Trypanosoma brucei gambiense"],"prefix":"10.1186","volume":"6","author":[{"given":"Nick","family":"Van Reet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pati","family":"Pyana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stijn","family":"Rog\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Claes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"B\u00fcscher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,7,15]]},"reference":[{"key":"999_CR1","doi-asserted-by":"publisher","first-page":"e1007","DOI":"10.1371\/journal.pntd.0001007","volume":"5","author":"PP Simarro","year":"2011","unstructured":"Simarro PP, Diarra A, Ruiz Postigo JA, Franco JR, Jannin JG: The human african trypanosomiasis control and surveillance programme of the world health organization 2000\u20132009: the way forward. PLoS Negl Trop Dis. 2011, 5: e1007-10.1371\/journal.pntd.0001007.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR2","doi-asserted-by":"publisher","first-page":"202","DOI":"10.4161\/viru.19178","volume":"3","author":"W Masocha","year":"2012","unstructured":"Masocha W, Kristensson K: Passage of parasites across the blood\u2013brain barrier. Virulence. 2012, 3: 202-212.","journal-title":"Virulence"},{"key":"999_CR3","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1756-3305-3-15","volume":"3","author":"D Steverding","year":"2010","unstructured":"Steverding D: The development of drugs for treatment of sleeping sickness: a historical review. Parasit Vectors. 2010, 3: 15-10.1186\/1756-3305-3-15.","journal-title":"Parasit Vectors"},{"key":"999_CR4","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.coph.2012.05.001","volume":"12","author":"P M\u00e4ser","year":"2012","unstructured":"M\u00e4ser P, Wittlin S, Rottmann M, Wenzler T, Kaiser M, Brun R: Antiparasitic agents: new drugs on the horizon. Curr Opin Pharmacol. 2012, 12: 562-566. 10.1016\/j.coph.2012.05.001.","journal-title":"Curr Opin Pharmacol"},{"key":"999_CR5","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1111\/j.1747-0285.2011.01156.x","volume":"78","author":"ZB Mackey","year":"2011","unstructured":"Mackey ZB, Koupparis K, Nishino M, McKerrow JH: High-throughput analysis of an RNAi library identifies novel kinase targets in Trypanosoma brucei. Chem Biol Drug Des. 2011, 78: 454-463. 10.1111\/j.1747-0285.2011.01156.x.","journal-title":"Chem Biol Drug Des"},{"key":"999_CR6","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.jep.2007.10.028","volume":"115","author":"GK Mesia","year":"2008","unstructured":"Mesia GK, Tona GL, Nanga TH, Cimanga RK, Apers S, Cos P, Maes L, Pieters L, Vlietinck AJ: Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo. J Ethnopharmacol. 2008, 115: 409-415. 10.1016\/j.jep.2007.10.028.","journal-title":"J Ethnopharmacol"},{"key":"999_CR7","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1186\/1756-3305-2-54","volume":"2","author":"ML Sykes","year":"2009","unstructured":"Sykes ML, Avery VM: A luciferase based viability assay for ATP detection in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427. Parasit Vectors. 2009, 2: 54-10.1186\/1756-3305-2-54.","journal-title":"Parasit Vectors"},{"key":"999_CR8","doi-asserted-by":"publisher","first-page":"665","DOI":"10.4269\/ajtmh.2009.09-0015","volume":"81","author":"ML Sykes","year":"2009","unstructured":"Sykes ML, Avery VM: Development of an Alamar Blue viability assay in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427. AmJTrop Med Hyg. 2009, 81: 665-674. 10.4269\/ajtmh.2009.09-0015.","journal-title":"AmJTrop Med Hyg"},{"key":"999_CR9","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.1016\/j.bcp.2010.07.038","volume":"80","author":"DC Jones","year":"2010","unstructured":"Jones DC, Hallyburton I, Stojanovski L, Read KD, Frearson JA, Fairlamb AH: Identification of a kappa-opioid agonist as a potent and selective lead for drug development against human African trypanosomiasis. Biochem Pharmacol. 2010, 80: 1478-1486. 10.1016\/j.bcp.2010.07.038.","journal-title":"Biochem Pharmacol"},{"key":"999_CR10","doi-asserted-by":"publisher","first-page":"e1023","DOI":"10.1371\/journal.pntd.0001023","volume":"5","author":"KK Ang","year":"2011","unstructured":"Ang KK, Ratnam J, Gut J, Legac J, Hansell E, Mackey ZB, Skrzypczynska KM, Debnath A, Engel JC, Rosenthal PJ: Mining a cathepsin inhibitor library for new antiparasitic drug leads. PLoS Negl Trop Dis. 2011, 5: e1023-10.1371\/journal.pntd.0001023.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR11","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1021\/jm201091t","volume":"55","author":"S Brand","year":"2012","unstructured":"Brand S, Cleghorn LA, McElroy SP, Robinson DA, Smith VC, Hallyburton I, Harrison JR, Norcross NR, Spinks D, Bayliss T: Discovery of a novel class of orally active trypanocidal N-myristoyltransferase inhibitors. J Med Chem. 2012, 55: 140-152. 10.1021\/jm201091t.","journal-title":"J Med Chem"},{"key":"999_CR12","doi-asserted-by":"publisher","first-page":"e46172","DOI":"10.1371\/journal.pone.0046172","volume":"7","author":"G Navarro","year":"2012","unstructured":"Navarro G, Chokpaiboon S, De MG, Bray WM, Nisam SC, McKerrow JH, Pudhom K, Linington RG: Hit-to-lead development of the chamigrane endoperoxide merulin A for the treatment of African sleeping sickness. PLoS One. 2012, 7: e46172-10.1371\/journal.pone.0046172.","journal-title":"PLoS One"},{"key":"999_CR13","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.ijpddr.2012.02.002","volume":"2","author":"T Bowling","year":"2012","unstructured":"Bowling T, Mercer L, Don R, Jacobs R, Nare B: Application of a resazurin-based high-throughput screening assay for the identification and progression of new treatments for human African trypanosomiasis. Int J Parasitol Drugs Drug Resist. 2012, 2: 262-270.","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"999_CR14","doi-asserted-by":"publisher","first-page":"e1932","DOI":"10.1371\/journal.pntd.0001932","volume":"6","author":"M De Rycker","year":"2012","unstructured":"De Rycker M, O\u2019Neill S, Joshi D, Campbell L, Gray DW, Fairlamb AH: A static-cidal assay for Trypanosoma brucei to aid hit prioritisation for progression into drug discovery programmes. PLoS Negl Trop Dis. 2012, 6: e1932-10.1371\/journal.pntd.0001932.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR15","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1128\/AAC.42.11.2858","volume":"42","author":"R Kaminsky","year":"1998","unstructured":"Kaminsky R, Brun R: In vitro and in vivo activities of trybizine hydrochloride against various pathogenic trypanosome species. Antimicrob Agents Chemother. 1998, 42: 2858-2862.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR16","doi-asserted-by":"publisher","first-page":"4185","DOI":"10.1128\/AAC.00225-09","volume":"53","author":"T Wenzler","year":"2009","unstructured":"Wenzler T, Boykin DW, Ismail MA, Hall JE, Tidwell RR, Brun R: New treatment option for second-stage African sleeping sickness: in vitro and in vivo efficacy of aza analogs of DB289. Antimicrob Agents Chemother. 2009, 53: 4185-4192. 10.1128\/AAC.00225-09.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR17","doi-asserted-by":"publisher","first-page":"4379","DOI":"10.1128\/AAC.00498-10","volume":"54","author":"B Nare","year":"2010","unstructured":"Nare B, Wring S, Bacchi C, Beaudet B, Bowling T, Brun R, Chen D, Ding C, Freund Y, Gaukel E: Discovery of novel orally bioavailable oxaborole 6-carboxamides that demonstrate cure in a murine model of late-stage central nervous system African trypanosomiasis. Antimicrob Agents Chemother. 2010, 54: 4379-4388. 10.1128\/AAC.00498-10.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR18","doi-asserted-by":"publisher","first-page":"e923","DOI":"10.1371\/journal.pntd.0000923","volume":"4","author":"E Torreele","year":"2010","unstructured":"Torreele E, Bourdin TB, Tweats D, Kaiser M, Brun R, Mazue G, Bray MA, Pecoul B: Fexinidazole - a new oral nitroimidazole drug candidate entering clinical development for the treatment of sleeping sickness. PLoS Negl Trop Dis. 2010, 4: e923-10.1371\/journal.pntd.0000923.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR19","doi-asserted-by":"publisher","first-page":"e1151","DOI":"10.1371\/journal.pntd.0001151","volume":"5","author":"RT Jacobs","year":"2011","unstructured":"Jacobs RT, Nare B, Wring SA, Orr MD, Chen D, Sligar JM, Jenks MX, Noe RA, Bowling TS, Mercer LT: SCYX-7158, an Orally-Active Benzoxaborole for the Treatment of Stage 2 Human African Trypanosomiasis. PLoS Negl Trop Dis. 2011, 5: e1151-10.1371\/journal.pntd.0001151.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR20","doi-asserted-by":"publisher","first-page":"5602","DOI":"10.1128\/AAC.00246-11","volume":"55","author":"M Kaiser","year":"2011","unstructured":"Kaiser M, Bray MA, Cal M, Bourdin TB, Torreele E, Brun R: Antitrypanosomal activity of fexinidazole, a new oral nitroimidazole drug candidate for treatment of sleeping sickness. Antimicrob Agents Chemother. 2011, 55: 5602-5608. 10.1128\/AAC.00246-11.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR21","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1128\/AAC.34.6.1183","volume":"34","author":"CJ Bacchi","year":"1990","unstructured":"Bacchi CJ, Nathan HC, Livingston T, Valladares G, Saric M, Sayer PD, Njogu AR, Clarkson AB: Differential susceptibility to DL-alpha-difluoromethylornithine in clinical isolates of Trypanosoma brucei rhodesiense. Antimicrob Agents Chemother. 1990, 34: 1183-1188. 10.1128\/AAC.34.6.1183.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR22","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1016\/j.trstmh.2006.11.008","volume":"101","author":"NW Maina","year":"2007","unstructured":"Maina NW, Oberle M, Otieno C, Kunz C, Maeser P, Ndung\u2019u JM, Brun R: Isolation and propagation of Trypanosoma brucei gambiense from sleeping sickness patients in south Sudan. Trans R Soc Trop Med Hyg. 2007, 101: 540-546. 10.1016\/j.trstmh.2006.11.008.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"999_CR23","doi-asserted-by":"publisher","first-page":"e1025","DOI":"10.1371\/journal.pntd.0001025","volume":"5","author":"PP Pyana","year":"2011","unstructured":"Pyana PP, Ngay Lukusa I, Mumba Ngoyi D, Van Reet N, Kaiser M, Karhemere Bin Shamamba S, B\u00fcscher P: Isolation of Trypanosoma brucei gambiense from cured and relapsed sleeping sickness patients and adaptation to laboratory mice. PLoS Negl Trop Dis. 2011, 5: e1025-10.1371\/journal.pntd.0001025.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR24","doi-asserted-by":"publisher","first-page":"e658","DOI":"10.1371\/journal.pntd.0000658","volume":"4","author":"AP Jackson","year":"2010","unstructured":"Jackson AP, Sanders M, Berry A, McQuillan J, Aslett MA, Quail MA, Chukualim B, Capewell P, MacLeod A, Melville SE: The genome sequence of Trypanosoma brucei gambiense, causative agent of chronic human African trypanosomiasis. PLoS Negl Trop Dis. 2010, 4: e658-10.1371\/journal.pntd.0000658.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR25","first-page":"190","volume":"46","author":"M Iten","year":"1995","unstructured":"Iten M, Matovu E, Brun R, Kaminsky R: Innate lack of susceptibility of Ugandan Trypanosoma brucei rhodesiense to DL-alfa-difluoromethylornithine (DFMO). Trop Med Parasitol. 1995, 46: 190-194.","journal-title":"Trop Med Parasitol"},{"key":"999_CR26","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/S0001-706X(97)00079-X","volume":"68","author":"B Raz","year":"1997","unstructured":"Raz B, Iten M, Grether-Buhler Y, Kaminsky R, Brun R: The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro. Acta Trop. 1997, 68: 139-147. 10.1016\/S0001-706X(97)00079-X.","journal-title":"Acta Trop"},{"key":"999_CR27","doi-asserted-by":"crossref","first-page":"195040","DOI":"10.1155\/2009\/195040","volume":"2009","author":"CJ Bacchi","year":"2009","unstructured":"Bacchi CJ: Chemotherapy of human african trypanosomiasis. Interdiscip Perspect Infect Dis. 2009, 2009: 195040-","journal-title":"Interdiscip Perspect Infect Dis"},{"key":"999_CR28","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.exppara.2011.02.018","volume":"128","author":"N Van Reet","year":"2011","unstructured":"Van Reet N, Pyana PP, Deborggraeve S, B\u00fcscher P, Claes F: Trypanosoma brucei gambiense: HMI-9 medium containing methylcellulose and human serum supports the continuous axenic in vitro propagation of the bloodstream form. Exp Parasitol. 2011, 128: 285-290. 10.1016\/j.exppara.2011.02.018.","journal-title":"Exp Parasitol"},{"key":"999_CR29","doi-asserted-by":"publisher","first-page":"e509","DOI":"10.1371\/journal.pntd.0000509","volume":"3","author":"C Giroud","year":"2009","unstructured":"Giroud C, Ottones F, Coustou V, Dacheux D, Biteau N, Miezan B, Van Reet N, Carrington M, Doua F, Baltz T: Murine models for Trypanosoma brucei gambiense disease progression-from silent to chronic infections and early brain tropism. PLoS Negl Trop Dis. 2009, 3: e509-10.1371\/journal.pntd.0000509.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR30","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/0001-706X(89)90049-1","volume":"46","author":"R Brun","year":"1989","unstructured":"Brun R, Baeriswyl S, Kunz C: In vitro drug sensitivity of Trypanosoma gambiense isolates. Acta Trop. 1989, 46: 369-376. 10.1016\/0001-706X(89)90049-1.","journal-title":"Acta Trop"},{"key":"999_CR31","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.ab.2008.07.036","volume":"382","author":"MK Gould","year":"2008","unstructured":"Gould MK, Vu XL, Seebeck T, de Koning HP: Propidium iodide-based methods for monitoring drug action in the Kinetoplastidae: comparison with the Alamar Blue assay. Anal Biochem. 2008, 382: 87-93. 10.1016\/j.ab.2008.07.036.","journal-title":"Anal Biochem"},{"key":"999_CR32","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1055\/s-2001-17351","volume":"67","author":"K Merschjohann","year":"2001","unstructured":"Merschjohann K, Sporer F, Steverding D, Wink M: In vitro effect of alkaloids on bloodstream forms of Trypanosoma brucei and T. congolense. Planta Med. 2001, 67: 623-627. 10.1055\/s-2001-17351.","journal-title":"Planta Med"},{"key":"999_CR33","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.exppara.2008.05.003","volume":"120","author":"L Cui","year":"2008","unstructured":"Cui L, Miao J, Wang J, Li Q, Cui L: Plasmodium falciparum: development of a transgenic line for screening antimalarials using firefly luciferase as the reporter. Exp Parasitol. 2008, 120: 80-87. 10.1016\/j.exppara.2008.05.003.","journal-title":"Exp Parasitol"},{"key":"999_CR34","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1089\/adt.2011.0378","volume":"10","author":"P Che","year":"2012","unstructured":"Che P, Cui L, Kutsch O, Cui L, Li Q: Validating a firefly luciferase-based high-throughput screening assay for antimalarial drug discovery. Assay Drug Dev Technol. 2012, 10: 61-68. 10.1089\/adt.2011.0378.","journal-title":"Assay Drug Dev Technol"},{"key":"999_CR35","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1128\/AAC.45.4.1168-1173.2001","volume":"45","author":"D Sereno","year":"2001","unstructured":"Sereno D, Roy G, Lemesre JL, Papadopoulou B, Ouellette M: DNA transformation of Leishmania infantum axenic amastigotes and their use in drug screening. Antimicrob Agents Chemother. 2001, 45: 1168-1173. 10.1128\/AAC.45.4.1168-1173.2001.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR36","doi-asserted-by":"publisher","first-page":"e1323","DOI":"10.1371\/journal.pntd.0001323","volume":"5","author":"G Michel","year":"2011","unstructured":"Michel G, Ferrua B, Lang T, Maddugoda MP, Munro P, Pomares C, Lemichez E, Marty P: Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro. PLoS Negl Trop Dis. 2011, 5: e1323-10.1371\/journal.pntd.0001323.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR37","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.actatropica.2011.11.015","volume":"122","author":"SA Pulido","year":"2012","unstructured":"Pulido SA, Munoz DL, Restrepo AM, Mesa CV, Alzate JF, Velez ID, Robledo SM: Improvement of the green fluorescent protein reporter system in Leishmania spp. for the in vitro and in vivo screening of antileishmanial drugs. Acta Trop. 2012, 122: 36-45. 10.1016\/j.actatropica.2011.11.015.","journal-title":"Acta Trop"},{"key":"999_CR38","doi-asserted-by":"publisher","first-page":"4246","DOI":"10.1128\/AAC.00800-10","volume":"54","author":"C Bot","year":"2010","unstructured":"Bot C, Hall BS, Bashir N, Taylor MC, Helsby NA, Wilkinson SR: Trypanocidal activity of aziridinyl nitrobenzamide prodrugs. Antimicrob Agents Chemother. 2010, 54: 4246-4252. 10.1128\/AAC.00800-10.","journal-title":"Antimicrob Agents Chemother"},{"key":"999_CR39","doi-asserted-by":"publisher","first-page":"e740","DOI":"10.1371\/journal.pntd.0000740","volume":"4","author":"AM Canavaci","year":"2010","unstructured":"Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson LJ, Xu D, Boehlke CL, Tarleton RL: In vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. PLoS Negl Trop Dis. 2010, 4: e740-10.1371\/journal.pntd.0000740.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR40","doi-asserted-by":"publisher","first-page":"e486","DOI":"10.1371\/journal.pntd.0000486","volume":"3","author":"F Claes","year":"2009","unstructured":"Claes F, Vodnala SK, Van Reet N, Boucher N, Lunden-Miguel H, Baltz T, Goddeeris BM, B\u00fcscher P, Rottenberg ME: Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness patients. PLoS Negl Trop Dis. 2009, 3: e486-10.1371\/journal.pntd.0000486.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR41","doi-asserted-by":"publisher","first-page":"e495","DOI":"10.1371\/journal.pntd.0000495","volume":"3","author":"SK Vodnala","year":"2009","unstructured":"Vodnala SK, Ferella M, Lunden-Miguel H, Betha E, Van Reet N, Amin DN, Oberg B, Andersson B, Kristensson K, Wigzell H: Preclinical assessment of the treatment of second-stage African trypanosomiasis with cordycepin and deoxycoformycin. PLoS Negl Trop Dis. 2009, 3: e495-10.1371\/journal.pntd.0000495.","journal-title":"PLoS Negl Trop Dis"},{"key":"999_CR42","doi-asserted-by":"publisher","first-page":"11183","DOI":"10.1128\/JVI.05468-11","volume":"85","author":"X Xie","year":"2011","unstructured":"Xie X, Wang QY, Xu HY, Qing M, Kramer L, Yuan Z, Shi PY: Inhibition of dengue virus by targeting viral NS4B protein. J Virol. 2011, 85: 11183-11195. 10.1128\/JVI.05468-11.","journal-title":"J Virol"},{"key":"999_CR43","doi-asserted-by":"publisher","first-page":"e28338","DOI":"10.1371\/journal.pone.0028338","volume":"6","author":"DF Gilbert","year":"2011","unstructured":"Gilbert DF, Erdmann G, Zhang X, Fritzsche A, Demir K, Jaedicke A, Muehlenberg K, Wanker EE, Boutros M: A novel multiplex cell viability assay for high-throughput RNAi screening. PLoS One. 2011, 6: e28338-10.1371\/journal.pone.0028338.","journal-title":"PLoS One"},{"key":"999_CR44","first-page":"53","volume":"262","author":"R McCulloch","year":"2004","unstructured":"McCulloch R, Vassella E, Burton P, Boshart M, Barry JD: Transformation of monomorphic and pleomorphic Trypanosoma brucei. Methods Mol Biol. 2004, 262: 53-86.","journal-title":"Methods Mol Biol"},{"key":"999_CR45","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1086\/649917","volume":"201","author":"D Mumba Ngoyi","year":"2010","unstructured":"Mumba Ngoyi D, Lejon V, Pyana P, Boelaert M, Ilunga M, Menten J, Mulunda JP, Van Nieuwenhove S, Muyembe Tamfum JJ, B\u00fcscher P: How to shorten patient follow-up after treatment for Trypanosoma brucei gambiense sleeping sickness?. J Infect Dis. 2010, 201: 453-463. 10.1086\/649917.","journal-title":"J Infect Dis"},{"key":"999_CR46","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","volume":"28","author":"SM Lanham","year":"1970","unstructured":"Lanham SM, Godfrey DG: Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol. 1970, 28: 521-534. 10.1016\/0014-4894(70)90120-7.","journal-title":"Exp Parasitol"},{"key":"999_CR47","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/0014-4894(76)90110-7","volume":"40","author":"WJ Herbert","year":"1976","unstructured":"Herbert WJ, Lumsden WHR: Trypanosoma brucei: A rapid \u201cmatching\u201d method for estimating the host\u2019s parasitemia. Exp Parasitol. 1976, 40: 427-431. 10.1016\/0014-4894(76)90110-7.","journal-title":"Exp Parasitol"},{"key":"999_CR48","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.vetpar.2007.05.020","volume":"148","author":"K Gillingwater","year":"2007","unstructured":"Gillingwater K, B\u00fcscher P, Brun R: Establishment of a panel of reference Trypanosoma evansi and Trypanosoma equiperdum strains for drug screening. Vet Parasitol. 2007, 148: 114-121. 10.1016\/j.vetpar.2007.05.020.","journal-title":"Vet Parasitol"},{"key":"999_CR49","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.molbiopara.2007.02.008","volume":"153","author":"G Burkard","year":"2007","unstructured":"Burkard G, Fragoso CM, Roditi I: Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei. Mol Biochem Parasitol. 2007, 153: 220-223. 10.1016\/j.molbiopara.2007.02.008.","journal-title":"Mol Biochem Parasitol"},{"key":"999_CR50","doi-asserted-by":"publisher","first-page":"e618","DOI":"10.1371\/journal.pntd.0000618","volume":"4","author":"V Coustou","year":"2010","unstructured":"Coustou V, Guegan F, Plazolles N, Baltz T: Complete in vitro life cycle of Trypanosoma congolense: development of genetic tools. PLoS Negl Trop Dis. 2010, 4: e618-10.1371\/journal.pntd.0000618.","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Parasites & Vectors"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1756-3305-6-207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1756-3305-6-207\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1756-3305-6-207.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T04:41:47Z","timestamp":1564548107000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/1756-3305-6-207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,15]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["999"],"URL":"https:\/\/doi.org\/10.1186\/1756-3305-6-207","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,15]]},"article-number":"207"}}