{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:22:07Z","timestamp":1790169727227,"version":"4.1.0"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Cutaneous leishmaniasis is an infectious disease caused by flagellate protozoa of the genus <jats:italic>Leishmania<\/jats:italic>. In Morocco, anthroponotic cutaneous leishmaniasis due to <jats:italic>Leishmania tropica<\/jats:italic> is considered as a public health problem, but its epidemiology has not been fully elucidated. The main objective of this study was to detect <jats:italic>Leishmania<\/jats:italic> infection in the vector, <jats:italic>Phlebotomus sergenti<\/jats:italic> and in human skin samples, in the El Hanchane locality, an emerging focus of cutaneous leishmaniasis in central Morocco.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>A total of 643 sand flies were collected using CDC miniature light traps and identified morphologically. <jats:italic>Leishmania<\/jats:italic> species were characterized by ITS1 PCR-RFLP and ITS1-5.8S rRNA gene nested-PCR of samples from 123 females of <jats:italic>Phlebotomus sergenti<\/jats:italic> and 7 cutaneous leishmaniasis patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The sand flies collected consisted of 9 species, 7 of which belonged to the genus <jats:italic>Phlebotomus<\/jats:italic> and two to the genus <jats:italic>Sergentomyia<\/jats:italic>. <jats:italic>Phlebotomus sergenti<\/jats:italic> was the most predominant (76.67%).<\/jats:p>\n            <jats:p>By ITS1 PCR-RFLP <jats:italic>Leishmania tropica<\/jats:italic> was found in three <jats:italic>Phlebotomus sergenti<\/jats:italic> females and four patients (4\/7). Using nested PCR <jats:italic>Leishmania tropica<\/jats:italic> was identified in the same three <jats:italic>Phlebotomus sergenti<\/jats:italic> females and all the 7 patients. The sequencing of the nested PCR products recognized 7 haplotypes, of which 6 have never been described.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>This is the first molecular detection and identification of <jats:italic>Leishmania tropica<\/jats:italic> in human skin samples and <jats:italic>Phlebotomus sergenti<\/jats:italic> in support of its vector status in El Hanchane. The finding of seven <jats:italic>Leishmania tropica<\/jats:italic> haplotypes underscores heterogeneity of this species at a high level in Morocco.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-6-217","type":"journal-article","created":{"date-parts":[[2013,7,26]],"date-time":"2013-07-26T15:13:12Z","timestamp":1374851592000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco"],"prefix":"10.1186","volume":"6","author":[{"given":"Malika","family":"Ajaoud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nargys","family":"Es-sette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salsabil","family":"Hamdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderahmane Laamrani","family":"El-Idrissi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Riyad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meryem","family":"Lemrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,7,26]]},"reference":[{"key":"1089_CR1","first-page":"365","volume":"1986","author":"JA Rioux","year":"1986","unstructured":"Rioux JA, Lanotte G, Petter F, Dereure J, Akalay O, Pratlong F, Velez ID, Fikri NB, Maazoun R, Denial M:Les leishmanioses cutan\u00e9es du bassin m\u00e9diterran\u00e9en occidental: de l'identification enzymatique \u00e0 l'analyse \u00e9co-\u00e9pid\u00e9miologique. 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