{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:30:54Z","timestamp":1778833854800,"version":"3.51.4"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Chagas disease is most often transmitted to humans by <jats:italic>Trypanosoma cruzi<\/jats:italic> infected triatomine bugs, and remains a significant cause of morbidity and mortality in Central and South America. Control of Chagas disease has relied mainly on vector eradication. However, development of insect resistance has prompted us to develop a paratransgenic strategy to control vectorial transmission of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic>. Here, the potential role of recombinant endoglucanases as anti-trypanosomal agents for paratransgenic application is examined. The surface of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> is covered by a thick coat of mucin-like glycoproteins that have been proposed to play a role in the binding of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> to the membrane surface of the vector gut. We hypothesize that disruption of these glycoconjugates could arrest parasite development in the vector and abort the transmission cycle. In this work, we examine the effects of recombinant <jats:italic>Arthrobacter luteus<\/jats:italic> \u03b2-1, 3-glucanase expressed via <jats:italic>Rhodococcus rhodnii<\/jats:italic> on <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> Sylvio II strain.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and results<\/jats:title>\n            <jats:p>The coding sequence for \u03b2-1, 3-glucanase was cloned in-frame to a heterologous promoter\/signal sequence from the <jats:italic>Mycobacterium kansasii<\/jats:italic> alpha antigen gene resident in an <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>\/<jats:italic>R<\/jats:italic>. <jats:italic>rhodnii<\/jats:italic> shuttle vector. The resulting construct was confirmed by sequencing, and electroporated into <jats:italic>R<\/jats:italic>. <jats:italic>rhodnii<\/jats:italic>. Expression products from positive clones were purified from log phase cultures followed by dialysis into physiological buffers. Lysates and media were quantitated by ELISA against rabbit antibody specific to \u03b2-1,3-glucanase. Glucanase-positive samples were applied to live <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> parasites in culture and viability accessed by spectrophotometric and fluorescent microscopic measurements. <jats:italic>R<\/jats:italic>. <jats:italic>rhodnii<\/jats:italic>-expressed \u03b2-1,3-glucanase exhibited toxicity against <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> compared to controls when applied at 5 and 10% of the total culture volume. The decrease in cell viability ranged from a maximum of 50% for the media treatments to 80% for the filtered lysates.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These results suggest that recombinant \u03b2-glucanase could be a powerful addition to the arsenal of effector molecules for paratransgenic control of Chagas disease. In future studies, the ability of \u03b2-glucanase to function in combination with other effector molecules will be explored. Dual targeting of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> should not only slow resistance but also permit synergistic or additive lethal effects on <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-3305-6-65","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T09:26:28Z","timestamp":1363253188000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Recombinant Arthrobacter \u03b2-1, 3-glucanase as a potential effector molecule for paratransgenic control of Chagas disease"],"prefix":"10.1186","volume":"6","author":[{"given":"Christo","family":"Jose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Wyss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annabeth","family":"Fieck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivy","family":"Hurwitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi","family":"Durvasula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,3,14]]},"reference":[{"key":"852_CR1","volume-title":"Chagas disease: Control and elimination","author":"World Health Organization","year":"2010","unstructured":"World Health Organization: Chagas disease: Control and elimination. 2010, Geneva: WHO, Report by the Secretariat, Sixty-third World Health Assembly"},{"key":"852_CR2","doi-asserted-by":"publisher","first-page":"e1136","DOI":"10.1371\/journal.pntd.0001136","volume":"5","author":"HB Tanowitz","year":"2011","unstructured":"Tanowitz HB, Weiss LM, Montgomery SP: Chagas disease has now gone global. 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