{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:03:41Z","timestamp":1763017421655},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1186\/1756-3305-7-305","type":"journal-article","created":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T11:35:05Z","timestamp":1404300905000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Molecular detection and identification of Leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern Morocco"],"prefix":"10.1186","volume":"7","author":[{"given":"Nargys","family":"Es-Sette","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malika","family":"Ajaoud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abderrahman","family":"Laamrani-Idrissi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fouad","family":"Mellouki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meryem","family":"Lemrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,7,2]]},"reference":[{"key":"1494_CR1","volume-title":"Direction de l\u2019\u00c9pid\u00e9miologie et de Lutte Contre les Maladies: Rap ann","author":"Moroccan Health Ministry","year":"2011","unstructured":"Moroccan Health Ministry: Etat d\u2019avancement des programmes de lutte contre les maladies parasitaires. Direction de l\u2019\u00c9pid\u00e9miologie et de Lutte Contre les Maladies: Rap ann. 2011"},{"key":"1494_CR2","first-page":"549","volume":"131","author":"JA Rioux","year":"1984","unstructured":"Rioux JA, Rispail P, Lanotte G, Lepart J: Phlebotomus bioclimates relations ecology of leishmaniasis epidemiological corollaries: The example of Morocco. Botanical News. 1984, 131: 549-557.","journal-title":"Botanical News"},{"key":"1494_CR3","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1186\/1756-3305-6-217","volume":"6","author":"M Ajaoud","year":"2013","unstructured":"Ajaoud M, Es-sette N, Hamdi S, El-Idrissi AL, Riyad M, Lemrani M: Detection and molecular typing of Leishmania tropica from Phlebotomus sergenti and lesions of cutaneous leishmaniasis in an emerging focus of Morocco. Parasit Vectors. 2013, 6: 217.","journal-title":"Parasit Vectors"},{"key":"1494_CR4","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.3201\/eid1309.060946","volume":"13","author":"M Rhajaoui","year":"2007","unstructured":"Rhajaoui M, Nasereddin A, Fellah H, Azmi K, Amarir F, Al-Jawabreh A, Ereqat S, Planer J, Abdeen Z: New clinico-epidemiologic profile of cutaneous leishmaniasis, Morocco. Emerg Infect Dis. 2007, 13: 1358-1360.","journal-title":"Emerg Infect Dis"},{"key":"1494_CR5","first-page":"3","volume":"4","author":"M Lemrani","year":"1999","unstructured":"Lemrani M, Nejjar N, Benslimane A: A new focus of cutaneous leishmaniasis due to Leishmania infantum in Northern Morocco. G Ital Med Trop. 1999, 4: 3-4.","journal-title":"G Ital Med Trop"},{"key":"1494_CR6","first-page":"179","volume":"14","author":"JA Rioux","year":"1996","unstructured":"Rioux JA, Mahjour J, Gallego M, Dereure J, Perieres J, Laamrani A, Riera C, Saddikiand A, Mouki B: Leishmaniose cutan\u00e9e humaine \u00e0 Leishmania infantum MON-24 au Maroc. Bull Soc Fr Parasitol. 1996, 14: 179-183.","journal-title":"Bull Soc Fr Parasitol"},{"key":"1494_CR7","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1051\/parasite\/1998054325","volume":"5","author":"R Nejjar","year":"1998","unstructured":"Nejjar R, Lemrani M, Malki A, Ibrahimy S, Amarouch H, Benslimane A: Canine leishmaniasis due to Leishmania infantum MON-1 in northern Morocco. Parasite. 1998, 5: 325-330.","journal-title":"Parasite"},{"key":"1494_CR8","doi-asserted-by":"publisher","first-page":"e1609","DOI":"10.1371\/journal.pntd.0001609","volume":"6","author":"E Giorgobiani","year":"2012","unstructured":"Giorgobiani E, Lawyer PG, Babuadze G, Dolidze N, Jochim RC, Tskhvaradze L, Kikaleishvili K, Kamhawi S: Incrimination of Phlebotomus kandelakii and Phlebotomus balcanicus as vectors of Leishmania infantum in Tbilisi, Georgia. PLoS Negl Trop Dis. 2012, 6: e1609.","journal-title":"PLoS Negl Trop Dis"},{"key":"1494_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1365-2915.1990.tb00255.x","volume":"4","author":"R Killick-Kendrick","year":"1990","unstructured":"Killick-Kendrick R: Phlebotomine vectors of the leishmaniases: a review. Med Vet Entomol. 1990, 4: 1-24.","journal-title":"Med Vet Entomol"},{"key":"1494_CR10","doi-asserted-by":"crossref","first-page":"19505","DOI":"10.2807\/ese.15.10.19505-en","volume":"15","author":"PD Ready","year":"2010","unstructured":"Ready PD: Leishmaniasis emergence in Europe. Euro Surveill. 2010, 15: 19505.","journal-title":"Euro Surveill"},{"key":"1494_CR11","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1051\/parasite\/1997042181","volume":"4","author":"N Guessous-Idrissi","year":"1997","unstructured":"Guessous-Idrissi N, Hamdani A, Rhalem A, Riyad M, Sahibi H, Dehbi F, Bichichi M, Essari A, Berrag B: Epidemiology of human visceral leishmaniasis in Taounate, a northern province of Morocco. Parasite. 1997, 4: 181-185.","journal-title":"Parasite"},{"key":"1494_CR12","first-page":"94","volume":"130C","author":"A Hmamouch","year":"2014","unstructured":"Hmamouch A, Fatima A, Hajiba F, Mbarek K, Khadija B, Mohamed R, Faiza S: Coexistence of Leishmania tropica and Leishmania infantum in Sefrou province, Morocco. Acta Trop. 2014, 130C: 94-99.","journal-title":"Acta Trop"},{"key":"1494_CR13","volume-title":"Direction de l\u2019\u00c9pid\u00e9miologie et de Lutte Contre les Maladies: Rap ann","author":"Moroccan Health Ministry","year":"2008","unstructured":"Moroccan Health Ministry: Etat d\u2019avancement des programmes de lutte contre les maladies parasitaires. Direction de l\u2019\u00c9pid\u00e9miologie et de Lutte Contre les Maladies: Rap ann. 2008"},{"key":"1494_CR14","doi-asserted-by":"publisher","first-page":"e77778","DOI":"10.1371\/journal.pone.0077778","volume":"8","author":"A Amro","year":"2013","unstructured":"Amro A, Hamdi S, Lemrani M, Mouna I, Mohammed H, Mostafa S, Rhajaoui M, Hamarsheh O, Schonian G: Moroccan Leishmania infantum: genetic diversity and population structure as revealed by multi-locus microsatellite typing. PLoS One. 2013, 8: e77778.","journal-title":"PLoS One"},{"key":"1494_CR15","first-page":"365","volume":"1986","author":"JA Rioux","year":"1986","unstructured":"Rioux JA, Lanotte G, Petter F, Dereure J, Akalay O, Pratlong F, Velez ID, Fikri NB, Maazoun R, Denial M: Les leishmanioses cutan\u00e9es du bassin m\u00e9diterran\u00e9en occidental: de l\u2019identification enzymatique \u00e0 l\u2019analyse \u00e9co-\u00e9pid\u00e9miologique. L\u2019exemple de trois \u201cfoyers \u201d, tunisien, marocain et fran\u00e7ais. Leishmania Taxonomie et phylogen\u00e8se Applications \u00e9co-\u00e9pid\u00e9miologiques. (CollintCNRS\/INSERM) IMEE, Montpellier. 1986, 1986: 365-395.","journal-title":"(CollintCNRS\/INSERM) IMEE, Montpellier"},{"key":"1494_CR16","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.actatropica.2007.10.005","volume":"105","author":"E Rossi","year":"2008","unstructured":"Rossi E, Bongiorno G, Ciolli E, Di Muccio T, Scalone A, Gramiccia M, Gradoni L, Maroli M: Seasonal phenology, host-blood feeding preferences and natural Leishmania infection of Phlebotomus perniciosus (Diptera, Psychodidae) in a high-endemic focus of canine leishmaniasis in Rome province, Italy. Acta Trop. 2008, 105: 158-165.","journal-title":"Acta Trop"},{"key":"1494_CR17","first-page":"255","volume":"45","author":"U Sharma","year":"2008","unstructured":"Sharma U, Singh S: Insect vectors of Leishmania: distribution, physiology and their control. J Vector Borne Dis. 2008, 45: 255-272.","journal-title":"J Vector Borne Dis"},{"key":"1494_CR18","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1128\/AEM.66.5.1933-1938.2000","volume":"66","author":"AM Aransay","year":"2000","unstructured":"Aransay AM, Scoulica E, Tselentis Y: Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol. 2000, 66: 1933-1938.","journal-title":"Appl Environ Microbiol"},{"key":"1494_CR19","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1051\/parasite\/2006131017","volume":"13","author":"G Perruolo","year":"2006","unstructured":"Perruolo G, Noris Rodriguez N, Feliciangeli MD: Isolation of Leishmania (Viannia) braziliensis from Lutzomyia spinicrassa (species group Verrucarum) Morales Osorno Mesa, Osorno and Hoyos 1969, in the Venezuelan Andean region. Parasite. 2006, 13: 17-22.","journal-title":"Parasite"},{"key":"1494_CR20","volume-title":"Guide Act","author":"Moroccan Health Ministry","year":"2010","unstructured":"Moroccan Health Ministry: Lutte Contre les Leishmanioses. Guide Act. 2010"},{"key":"1494_CR21","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","volume":"162","author":"P Chomczynski","year":"1987","unstructured":"Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162: 156-159.","journal-title":"Anal Biochem"},{"key":"1494_CR22","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.actatropica.2004.09.007","volume":"93","author":"P Parvizi","year":"2005","unstructured":"Parvizi P, Mauricio I, Aransay AM, Miles MA, Ready PD: First detection of Leishmania major in peridomestic Phlebotomus papatasi from Isfahan province, Iran: comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast DNA. Acta Trop. 2005, 93: 75-83.","journal-title":"Acta Trop"},{"key":"1494_CR23","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1111\/j.1365-3156.2008.02121.x","volume":"13","author":"P Parvizi","year":"2008","unstructured":"Parvizi P, Ready PD: Nested PCRs and sequencing of nuclear ITS-rDNA fragments detect three Leishmania species of gerbils in sandflies from Iranian foci of zoonotic cutaneous leishmaniasis. Trop Med Int Health. 2008, 13: 1159-1171.","journal-title":"Trop Med Int Health"},{"key":"1494_CR24","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1093\/molbev\/mst197","volume":"30","author":"K Tamura","year":"2013","unstructured":"Tamura K, Stecher G, Peterson D, Filipski A, Kumar S: MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013, 30: 2725-2729.","journal-title":"Mol Biol Evol"},{"key":"1494_CR25","first-page":"406","volume":"4","author":"M Saitou NaN","year":"1987","unstructured":"Saitou NaN M: The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987, 4: 406-425.","journal-title":"Mol Biol Evol"},{"key":"1494_CR26","doi-asserted-by":"publisher","first-page":"783","DOI":"10.2307\/2408678","volume":"39","author":"J Felsenstein","year":"1985","unstructured":"Felsenstein J: Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985, 39: 783-791.","journal-title":"Evolution"},{"key":"1494_CR27","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1046\/j.1365-2915.1999.00193.x","volume":"13","author":"DA Boakye","year":"1999","unstructured":"Boakye DA, Tang J, Truc P, Merriweather A, Unnasch TR: Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis. Med Vet Entomol. 1999, 13: 282-287.","journal-title":"Med Vet Entomol"},{"key":"1494_CR28","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1016\/0035-9203(90)90120-4","volume":"84","author":"JA Rioux","year":"1990","unstructured":"Rioux JA, Lanotte G: Leishmania infantum as a cause of cutaneous leishmaniasis. Trans R Soc Trop Med Hyg. 1990, 84: 898.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"1494_CR29","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/0035-9203(88)90142-3","volume":"82","author":"MA Al-Zahrani","year":"1988","unstructured":"Al-Zahrani MA, Peters W, Evans DA, Chin C, Smith V, Lane RP: Phlebotomus sergenti, a vector of Leishmania tropica in Saudi Arabia. Trans R Soc Trop Med Hyg. 1988, 82: 416.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"1494_CR30","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1051\/parasite\/199166396","volume":"66","author":"E Guilvard","year":"1991","unstructured":"Guilvard E, Rioux JA, Gallego M, Pratlong F, Mahjour J, Martinez-Ortega E, Dereure J, Saddiki A, Martini A: Leishmania tropica au Maroc. III. R\u00f4le de Phlebotomus sergenti. A propros de 89 isolats. Ann Parasitol Hum Comp. 1991, 66: 96-99.","journal-title":"Ann Parasitol Hum Comp"},{"key":"1494_CR31","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/j.micinf.2011.02.008","volume":"13","author":"SC1 Boubidi","year":"2011","unstructured":"Boubidi SC1, Benallal K, Boudrissa A, Bouiba L, Bouchareb B, Garni R, Bouratbine A, Ravel C, Dvorak V, Votypka J, Volf P, Harrat Z: Phlebotomus sergenti (Parrot, 1917) identified as Leishmania killicki host in Ghardaia, south Algeria. Microbes Infect. 2011, 13: 691-696.","journal-title":"Microbes Infect"},{"key":"1494_CR32","doi-asserted-by":"publisher","first-page":"646","DOI":"10.4269\/ajtmh.2011.10-0681","volume":"85","author":"A Tabbabi","year":"2011","unstructured":"Tabbabi A, Bousslimi N, Rhim A, Aoun K, Bouratbine A: First report on natural infection of Phlebotomus sergenti with Leishmania promastigotes in the cutaneous leishmaniasis focus in southeastern Tunisia. Am J Trop Med Hyg. 2011, 85: 646-647.","journal-title":"Am J Trop Med Hyg"},{"key":"1494_CR33","first-page":"146","volume":"30","author":"L Parrot","year":"1952","unstructured":"Parrot L, Donatien A: [New observations on the natural infection of Phlebotomus by general leishmaniasis of man and dog in Algeria]. Arch Inst Pasteur Alger. 1952, 30: 146-152.","journal-title":"Arch Inst Pasteur Alger"},{"key":"1494_CR34","first-page":"212","volume":"96","author":"Z Harrat","year":"2003","unstructured":"Harrat Z, Belkaid M: Leishmaniasis in Algiers: epidemiologic data. Bull Soc Pathol Exot. 2003, 96: 212-214.","journal-title":"Bull Soc Pathol Exot"},{"key":"1494_CR35","first-page":"421","volume":"1986","author":"J Dereure","year":"1984","unstructured":"Dereure J, Velez ID, Pratlong F, Denial M, Lardi M, Moreno G, Serres E, Lanotte G, Rioux JP: La leishmaniose viscerale autochtone au Maroc m\u00e9eridional. Pr\u00e9sence de Leishmania infantum MON-1 chez le Chien en zone presaharienne. Leishmania Taxonomie et Phylogenese Applications \u00e9co- \u00e9pid\u00e9miologiques. (Coll Int CNRS\/INSERM). 1984, 1986: 421-425.","journal-title":"Leishmania Taxonomie et Phylogenese Applications \u00e9co- \u00e9pid\u00e9miologiques. (Coll Int CNRS\/INSERM)"},{"key":"1494_CR36","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4269\/ajtmh.2005.72.87","volume":"72","author":"H Kato","year":"2005","unstructured":"Kato H, Uezato H, Katakura K, Calvopi\u00f1a M, Marco JD, Barroso PA, Gomez EA, Mimori T, Korenaga M, Iwata H, Nonaka S, Hashiguchi Y: Detection and identification of Leishmania species within naturally infected sand flies in the andean areas of ecuador by a polymerase chain reaction. Am J Trop Med Hyg. 2005, 72: 87-93.","journal-title":"Am J Trop Med Hyg"},{"key":"1494_CR37","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1186\/1756-3305-6-258","volume":"6","author":"VM Scarpassa","year":"2013","unstructured":"Scarpassa VM, Alencar RB: Molecular taxonomy of the two Leishmania vectors Lutzomyia umbratilis and Lutzomyia anduzei (Diptera: Psychodidae) from the Brazilian Amazon. Parasit Vectors. 2013, 6: 258.","journal-title":"Parasit Vectors"},{"key":"1494_CR38","doi-asserted-by":"publisher","first-page":"e55738","DOI":"10.1371\/journal.pone.0055738","volume":"8","author":"N Singh","year":"2013","unstructured":"Singh N, Chikara S, Sundar S: SOLiD\u2122 sequencing of genomes of clinical isolates of Leishmania donovani from India confirm leptomonas co-infection and raise some key questions. PLoS One. 2013, 8: e55738.","journal-title":"PLoS One"},{"key":"1494_CR39","doi-asserted-by":"publisher","first-page":"e2205","DOI":"10.1371\/journal.pntd.0002205","volume":"7","author":"SO Toz","year":"2013","unstructured":"Toz SO, Culha G, Zeyrek FY, Ertabaklar H, Alkan MZ, Vardarli AT, Gunduz C, Ozbel Y: A real-time ITS1-PCR based method in the diagnosis and species identification of Leishmania parasite from human and dog clinical samples in Turkey. PLoS Negl Trop Dis. 2013, 7: e2205.","journal-title":"PLoS Negl Trop Dis"},{"key":"1494_CR40","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1093\/oxfordjournals.molbev.a003825","volume":"18","author":"J Castresana","year":"2001","unstructured":"Castresana J: Cytochrome b phylogeny and the taxonomy of great apes and mammals. Mol Biol Evol. 2001, 18: 465-471.","journal-title":"Mol Biol Evol"},{"key":"1494_CR41","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1603\/0022-2585-40.2.215","volume":"40","author":"KA Ngo","year":"2003","unstructured":"Ngo KA, Kramer LD: Identification of mosquito bloodmeals using polymerase chain reaction (PCR) with order-specific primers. J Med Entomol. 2003, 40: 215-222.","journal-title":"J Med Entomol"},{"key":"1494_CR42","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/1756-3305-6-32","volume":"6","author":"BB Yang","year":"2013","unstructured":"Yang BB, Chen DL, Chen JP, Liao L, Hu XS, Xu JN: Analysis of kinetoplast cytochrome b gene of 16 Leishmania isolates from different foci of China: different species of Leishmania in China and their phylogenetic inference. Parasit Vectors. 2013, 6: 32.","journal-title":"Parasit Vectors"},{"key":"1494_CR43","first-page":"841","volume":"151","author":"R Nejjar","year":"2000","unstructured":"Nejjar R, Lemrani M, Amarouche A, Benslimane A: Variation in antibody titres against Leishmania infantum in naturally infected dogs in northern Morocco. Rev Med Vet. 2000, 151: 841-846.","journal-title":"Rev Med Vet"}],"container-title":["Parasites & Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1756-3305-7-305.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T04:56:30Z","timestamp":1630558590000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/1756-3305-7-305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,2]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["1494"],"URL":"https:\/\/doi.org\/10.1186\/1756-3305-7-305","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,2]]},"assertion":[{"value":"28 March 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"305"}}