{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T19:40:08Z","timestamp":1685562008602},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Hematol Oncol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The development of Philadelphia chromosome (Ph) negative acute leukemia\/myelodysplastic syndrome (MDS) in patients with Ph-positive chronic myeloid leukemia (CML) is very rare. The features of restrictive usage and absence of partial T cell clones have been found in patients with CML. However, the T-cell clonal evolution of Ph-negative malignancies during treatment for CML is still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the dynamic change of clonal proliferation of T cell receptor (TCR) V\u03b1 and V\u03b2 subfamilies in one CML patient who developed Ph-negative acute lymphoblastic leukemia (ALL) after interferon and imatinib therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The peripheral blood mononuclear cells (PBMC) samples were collected at the 3 time points (diagnosis of Ph-positive chronic phase (CP) CML, developing Ph-negative ALL and post inductive chemotherapy (CT) for Ph-negative ALL, respectively). The CDR3 size of TCR V\u03b1 and V\u03b2 repertoire were detected by RT-PCR. The PCR products were further analyzed by genescan to identify T cell clonality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The CML patient who achieved complete cytogenetic remission (CCR) after 5 years of IFN-\u03b1 therapy suddenly developed Ph-negative ALL 6 months following switch to imatinib therapy. The expression pattern and clonality of TCR V\u03b1\/V\u03b2 T cells changed in different disease stages. The restrictive expression of V\u03b1\/V\u03b2 subfamilies could be found in all three stages, and partial subfamily of T cells showed clonal proliferation. Additionally, there have been obvious differences in V\u03b1\/V\u03b2 subfamily of T cells between the stages of Ph-positive CML-CP and Ph-negative ALL. The V\u03b110 and V\u03b23 T cells evolved from oligoclonality to polyclonality, the V\u03b213 T cells changed from bioclonality to polyclonality, when Ph-negative ALL developed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Restrictive usage and clonal proliferation of different V\u03b1\/V\u03b2 subfamily T cells between the stages of Ph-positive CP and Ph-negative ALL were detected in one patient. These changes may play a role in Ph- negative leukemogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1756-8722-3-14","type":"journal-article","created":{"date-parts":[[2010,4,9]],"date-time":"2010-04-09T06:14:04Z","timestamp":1270793644000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia"],"prefix":"10.1186","volume":"3","author":[{"given":"Liang","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanger","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianfeng","family":"Zha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangqiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,4,9]]},"reference":[{"key":"98_CR1","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1159\/000046636","volume":"107","author":"B Johansson","year":"2002","unstructured":"Johansson B, Fioretos T, Mitelman F: Cytogenetic and molecular genetic evolution of chronic myeloid leukemia. Acta Haematol. 2002, 107: 76-94. 10.1159\/000046636.","journal-title":"Acta Haematol"},{"key":"98_CR2","doi-asserted-by":"publisher","first-page":"2811","DOI":"10.1182\/blood-2006-04-017400","volume":"108","author":"C Kovitz","year":"2006","unstructured":"Kovitz C, Kantarjian H, Garcia-Manero G, Abruzzo LV, Cortes J: Myelodysplastic syndromes and acute leukemia developing after imatinib mesylate therapy for chronic myeloid leukemia. Blood. 2006, 108: 2811-2813. 10.1182\/blood-2006-04-017400.","journal-title":"Blood"},{"key":"98_CR3","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1046\/j.1365-2141.2001.02960.x","volume":"114","author":"WY Au","year":"2001","unstructured":"Au WY, Lie AK, Ma SK, Wan TS, Liang R, Leung YH, Kwong YL: Philadelphia (Ph) chromosome-positive chronic myeloid leukaemia relapsing as Ph-negative leukaemia after allogeneic bone marrow transplantation. Br J Haematol. 2001, 114: 365-368. 10.1046\/j.1365-2141.2001.02960.x.","journal-title":"Br J Haematol"},{"key":"98_CR4","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1159\/000203611","volume":"98","author":"S Bose","year":"1997","unstructured":"Bose S, Chowdhry VP, Saxena R, Kucheria K: Lymphoid blast crisis during complete cytogenetic remission following interferon-alpha and hydroxyurea therapy. Acta Haematol. 1997, 98: 155-159. 10.1159\/000203611.","journal-title":"Acta Haematol"},{"key":"98_CR5","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/S0165-4608(03)00277-2","volume":"148","author":"MJ Cullen","year":"2004","unstructured":"Cullen MJ, Richards SJ, Dickinson H, Carter C, Swirsky DM, Owen RG: Development and progression of a Philadelphia-chromosome-negative acute myelocytic leukemia clone in a patient with Philadelphia-chromosome-positive chronic myelocytic leukemia. Cancer Genet Cytogenet. 2004, 148: 170-173. 10.1016\/S0165-4608(03)00277-2.","journal-title":"Cancer Genet Cytogenet"},{"key":"98_CR6","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1046\/j.1365-2141.1999.01476.x","volume":"106","author":"L Dawson","year":"1999","unstructured":"Dawson L, Slater R, Hagemeijer A, Langerak AW, Willemze R, Kluin-Nelemans JC: Secondary T-acute lymphoblastic leukaemia mimicking blast crisis in chronic myeloid leukaemia. Br J Haematol. 1999, 106: 104-106. 10.1046\/j.1365-2141.1999.01476.x.","journal-title":"Br J Haematol"},{"key":"98_CR7","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/S0145-2126(01)00140-0","volume":"26","author":"MJ Flamm","year":"2002","unstructured":"Flamm MJ, Murty VV, Rao PH, Nichols GL: Coexistence of independent myelodysplastic and Philadelphia chromosome positive clones in a patient treated with hydroxyurea. Leuk Res. 2002, 26: 417-420. 10.1016\/S0145-2126(01)00140-0.","journal-title":"Leuk Res"},{"key":"98_CR8","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/sj.leu.2401220","volume":"13","author":"R Manley","year":"1999","unstructured":"Manley R, Cochrane J, McDonald M, Rigby S, Moore A, Kirk A, Clarke S, Crossen PE, Morris CM, Patton WN: Clonally unrelated BCR-ABL-negative acute myeloblastic leukemia masquerading as blast crisis after busulphan and interferon therapy for BCR-ABL-positive chronic myeloid leukemia. Leukemia. 1999, 13: 126-129. 10.1038\/sj\/leu\/2401220.","journal-title":"Leukemia"},{"key":"98_CR9","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1111\/j.1365-2141.1995.tb05223.x","volume":"90","author":"E Ohtsuka","year":"1995","unstructured":"Ohtsuka E, Kikuchi H, Abe Y, Moriyama K, Ohno E, Hirota K, Tezono K, Nasu M: Acute myeloblastic leukaemia without Philadelphia chromosome developing after interferon therapy for chronic myelocytic leukaemia with Philadelphia chromosome. Br J Haematol. 1995, 90: 951-953. 10.1111\/j.1365-2141.1995.tb05223.x.","journal-title":"Br J Haematol"},{"key":"98_CR10","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/S0145-2126(02)00175-3","volume":"27","author":"X Zhang","year":"2003","unstructured":"Zhang X, Ji L, Liu S, Wang J: Ph-negative acute lymphocytic leukemia occurring after interferon therapy for Ph-positive chronic myelocytic leukemia. Leuk Res. 2003, 27: 367-369. 10.1016\/S0145-2126(02)00175-3.","journal-title":"Leuk Res"},{"key":"98_CR11","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1034\/j.1600-0609.2003.00065.x","volume":"70","author":"RT Costello","year":"2003","unstructured":"Costello RT, Rey J, Fauriat C, Gastaut JA, Olive D: New approaches in the immunotherapy of haematological malignancies. Eur J Haematol. 2003, 70: 333-345. 10.1034\/j.1600-0609.2003.00065.x.","journal-title":"Eur J Haematol"},{"key":"98_CR12","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1016\/S1567-5769(03)00060-2","volume":"3","author":"JW Hadden","year":"2003","unstructured":"Hadden JW: Immunodeficiency and cancer: prospects for correction. Int Immunopharmacol. 2003, 3: 1061-1071. 10.1016\/S1567-5769(03)00060-2.","journal-title":"Int Immunopharmacol"},{"key":"98_CR13","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/0167-5699(95)80117-0","volume":"16","author":"C Pannetier","year":"1995","unstructured":"Pannetier C, Even J, Kourilsky P: T-cell repertoire diversity and clonal expansions in normal and clinical samples. Immunol Today. 1995, 16: 176-181. 10.1016\/0167-5699(95)80117-0.","journal-title":"Immunol Today"},{"key":"98_CR14","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/S1357-4310(98)01280-5","volume":"4","author":"P Romero","year":"1998","unstructured":"Romero P, Cerottini JC, Waanders GA: Novel methods to monitor antigen-specific cytotoxic T-cell responses in cancer immunotherapy. Mol Med Today. 1998, 4: 305-312. 10.1016\/S1357-4310(98)01280-5.","journal-title":"Mol Med Today"},{"key":"98_CR15","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1080\/10245330310001621288","volume":"8","author":"Y Li","year":"2003","unstructured":"Li Y: Leukemia associated clonal expansion of TCR Vbeta subfamily T cells. Hematology. 2003, 8: 375-384. 10.1080\/10245330310001621288.","journal-title":"Hematology"},{"key":"98_CR16","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1080\/10245330500226662","volume":"10","author":"Y Li","year":"2005","unstructured":"Li Y, Yang L, Chen S, Zhang Y, Wu X: The TCR Vbeta repertoire usage of T-cells from cord blood induced by chronic myelogenous leukemia associated antigen. Hematology. 2005, 10: 387-392. 10.1080\/10245330500226662.","journal-title":"Hematology"},{"key":"98_CR17","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1182\/blood.V94.3.1063.415a17_1063_1069","volume":"94","author":"MR Rezvany","year":"1999","unstructured":"Rezvany MR, Jeddi-Tehrani M, Osterborg A, Kimby E, Wigzell H, Mellstedt H: Oligoclonal TCRBV gene usage in B-cell chronic lymphocytic leukemia: major perturbations are preferentially seen within the CD4 T-cell subset. Blood. 1999, 94: 1063-1069.","journal-title":"Blood"},{"key":"98_CR18","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1182\/blood-2002-03-0746","volume":"101","author":"MR Rezvany","year":"2003","unstructured":"Rezvany MR, Jeddi-Tehrani M, Wigzell H, Osterborg A, Mellstedt H: Leukemia-associated monoclonal and oligoclonal TCR-BV use in patients with B-cell chronic lymphocytic leukemia. Blood. 2003, 101: 1063-1070. 10.1182\/blood-2002-03-0746.","journal-title":"Blood"},{"key":"98_CR19","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1034\/j.1600-065X.2002.18810.x","volume":"188","author":"F Mami-Chouaib","year":"2002","unstructured":"Mami-Chouaib F, Echchakir H, Dorothee G, Vergnon I, Chouaib S: Antitumor cytotoxic T-lymphocyte response in human lung carcinoma: identification of a tumor-associated antigen. Immunol Rev. 2002, 188: 114-121. 10.1034\/j.1600-065X.2002.18810.x.","journal-title":"Immunol Rev"},{"key":"98_CR20","doi-asserted-by":"crossref","first-page":"443","DOI":"10.4049\/jimmunol.163.1.443","volume":"163","author":"P thor Straten","year":"1999","unstructured":"thor Straten P, Guldberg P, Gronbaek K, Hansen MR, Kirkin AF, Seremet T, Zeuthen J, Becker JC: In situ T cell responses against melanoma comprise high numbers of locally expanded T cell clonotypes. J Immunol. 1999, 163: 443-447.","journal-title":"J Immunol"},{"key":"98_CR21","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.4049\/jimmunol.158.3.1482","volume":"158","author":"D Serrano","year":"1997","unstructured":"Serrano D, Monteiro J, Allen SL, Kolitz J, Schulman P, Lichtman SM, Buchbinder A, Vinciguerra VP, Chiorazzi N, Gregersen PK: Clonal expansion within the CD4+CD57+ and CD8+CD57+ T cell subsets in chronic lymphocytic leukemia. J Immunol. 1997, 158: 1482-1489.","journal-title":"J Immunol"},{"key":"98_CR22","doi-asserted-by":"publisher","first-page":"5698","DOI":"10.1073\/pnas.85.15.5698","volume":"85","author":"ES Kawasaki","year":"1988","unstructured":"Kawasaki ES, Clark SS, Coyne MY, Smith SD, Champlin R, Witte ON, McCormick FP: Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc Natl Acad Sci USA. 1988, 85: 5698-5702. 10.1073\/pnas.85.15.5698.","journal-title":"Proc Natl Acad Sci USA"},{"key":"98_CR23","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.trim.2007.05.011","volume":"18","author":"Y Li","year":"2007","unstructured":"Li Y, Chen S, Yang L, Yin Q, Geng S, Wu X, Schmidt CA, Przybylski GK: TRAV and TRBV repertoire, clonality and the proliferative history of umbilical cord blood T-cells. Transpl Immunol. 2007, 18: 151-158. 10.1016\/j.trim.2007.05.011.","journal-title":"Transpl Immunol"},{"key":"98_CR24","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.4049\/jimmunol.153.6.2807","volume":"153","author":"I Puisieux","year":"1994","unstructured":"Puisieux I, Even J, Pannetier C, Jotereau F, Favrot M, Kourilsky P: Oligoclonality of tumor-infiltrating lymphocytes from human melanomas. J Immunol. 1994, 153: 2807-2818.","journal-title":"J Immunol"},{"key":"98_CR25","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1038\/sj.leu.2400642","volume":"11","author":"L Fayad","year":"1997","unstructured":"Fayad L, Kantarjian H, O'Brien S, Seong D, Albitar M, Keating M, Talpaz M: Emergence of new clonal abnormalities following interferon-alpha induced complete cytogenetic response in patients with chronic myeloid leukemia: report of three cases. Leukemia. 1997, 11: 767-771. 10.1038\/sj.leu.2400642.","journal-title":"Leukemia"},{"key":"98_CR26","first-page":"840","volume":"117","author":"YQ Li","year":"2004","unstructured":"Li YQ, Yang LJ, Chen SH, Zhang YP, Zhang XL, Luo GX: T cell receptor Vbeta repertoire usage and clonal expansion of T cells in chronic myelogenous leukemia. Chin Med J (Engl). 2004, 117: 840-843.","journal-title":"Chin Med J (Engl)"}],"container-title":["Journal of Hematology &amp; Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1756-8722-3-14.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T19:06:20Z","timestamp":1685559980000},"score":1,"resource":{"primary":{"URL":"https:\/\/jhoonline.biomedcentral.com\/articles\/10.1186\/1756-8722-3-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,9]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["98"],"URL":"https:\/\/doi.org\/10.1186\/1756-8722-3-14","relation":{},"ISSN":["1756-8722"],"issn-type":[{"value":"1756-8722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,9]]},"assertion":[{"value":"2 December 2009","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2010","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 April 2010","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"14"}}