{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:22:55Z","timestamp":1773289375366,"version":"3.50.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Hematol Oncol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Our previous study had amplified antigen-specific full-length TCR \u03b1 and \u03b2 genes of clonally expanded T cells in the peripheral blood (PB) of patients with diffuse large B-cell lymphoma (DLBCL). The transfer of T cell receptor (TCR) genes endows T cells with new antigen specificity. Therefore, the aim of this study is to generate diffuse large B cell lymphoma (DLBCL)-specific T cells by T cell receptor (TCR) gene transfer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Two different eukaryotic expression plasmids harboring TCR V\u03b16 and TCR V\u03b213 genes specific for DLBCL-associated antigens were constructed and subsequently transferred into human T cells using Nucleofector\u2122 technique. The expression of targeted genes in TCR gene-modified cells was detected by real-time PCR, and western blot using TCR V\u03b2 antibody. The specific cytotoxicity of TCR gene-transferred T cells<jats:italic>in vitro<\/jats:italic>was estimated using a lactate dehydrogenase (LDH) release assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two different eukaryotic expression plasmids harboring TCR V\u03b16 and TCR V\u03b213 genes specific for DLBCL-associated antigens were constructed and subsequently transferred into T cells from healthy donors. Specific anti-DLBCL cytotoxic T lymphocytes (CTL) could be induced by transduction of specific TCR gene to modify healthy T cells. The transgene cassette of TCR V\u03b213-IRES-TCR V\u03b16 was superior to the other in the function of TCR-redirected T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Specific anti-DLBCL cytotoxic T lymphocyte (CTL) could be inducted by transduction of specific TCR gene to modify healthy T cells.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1756-8722-4-2","type":"journal-article","created":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T15:36:21Z","timestamp":1297179381000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Generation of diffuse large B cell lymphoma-associated antigen-specific V\u03b16\/V\u03b213+T cells by TCR gene transfer"],"prefix":"10.1186","volume":"4","author":[{"given":"Qingsong","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianfeng","family":"Zha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yubing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangqiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,1,11]]},"reference":[{"key":"136_CR1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S1074-7613(00)80028-X","volume":"10","author":"SA Rosenberg","year":"1999","unstructured":"Rosenberg SA: A new era for cancer immunotherapy based on the genes that encode cancer antigens. 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