{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:09:43Z","timestamp":1789204183787,"version":"build-2803163510"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Hematol Oncol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Downregulation of the B-cell chronic lymphocytic leukemia (CLL)\/lymphoma11B (<jats:italic>BCL11B)<\/jats:italic> gene by small interfering RNA (siRNA) leads to growth inhibition and apoptosis of the human T-cell acute lymphoblastic leukemia (T-ALL) cell line Molt-4. To further characterize the molecular mechanism, a global gene expression profile of <jats:italic>BCL11B<\/jats:italic>-siRNA -treated Molt-4 cells was established. The expression profiles of several genes were further validated in the <jats:italic>BCL11B<\/jats:italic>-siRNA -treated Molt-4 cells and primary T-ALL cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>142 genes were found to be upregulated and 109 genes downregulated in the <jats:italic>BCL11B<\/jats:italic>-siRNA -treated Molt-4 cells by microarray analysis. Among apoptosis-related genes, three pro-apoptotic genes, <jats:italic>TNFSF<\/jats:italic> 10, <jats:italic>BIK<\/jats:italic>, <jats:italic>BNIP<\/jats:italic> 3, were upregulated and one anti-apoptotic gene, <jats:italic>BCL2L<\/jats:italic> 1 was downregulated. Moreover, the expression of <jats:italic>SPP<\/jats:italic> 1 and <jats:italic>CREBBP<\/jats:italic> genes involved in the transforming growth factor (TGF-\u03b2) pathway was down 16-fold. Expression levels of <jats:italic>TNFSF<\/jats:italic> 10, <jats:italic>BCL2L<\/jats:italic> 1, <jats:italic>SPP<\/jats:italic> 1, and <jats:italic>CREBBP<\/jats:italic> were also examined by real-time PCR. A similar expression pattern of <jats:italic>TNFSF<\/jats:italic> 10, <jats:italic>BCL2L<\/jats:italic> 1, and <jats:italic>SPP<\/jats:italic> 1 was identified. However, <jats:italic>CREBBP<\/jats:italic> was not downregulated in the <jats:italic>BLC11B<\/jats:italic>-siRNA -treated Molt-4 cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>\n              <jats:italic>BCL11B<\/jats:italic>-siRNA treatment altered expression profiles of <jats:italic>TNFSF<\/jats:italic> 10, <jats:italic>BCL2L<\/jats:italic> 1, and <jats:italic>SPP<\/jats:italic> 1 in both Molt-4 T cell line and primary T-ALL cells.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1756-8722-4-23","type":"journal-article","created":{"date-parts":[[2011,5,17]],"date-time":"2011-05-17T18:19:12Z","timestamp":1305656352000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Gene expression profiles in BCL11B-siRNA treated malignant T cells"],"prefix":"10.1186","volume":"4","author":[{"given":"Xin","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Grabarczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz K","family":"Przybylski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian A","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangqiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,5,15]]},"reference":[{"key":"156_CR1","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1038\/nri2304","volume":"8","author":"I Aifantis","year":"2008","unstructured":"Aifantis I, Raetz E, Buonamici S: Molecular pathogenesis of T-cell leukaemia and lymphoma. 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