{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T17:38:33Z","timestamp":1765993113222},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,11,11]],"date-time":"2009-11-11T00:00:00Z","timestamp":1257897600000},"content-version":"vor","delay-in-days":283,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of the International AIDS Society"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The central nervous system is considered a sanctuary site for HIV\u20101 replication. Variables associated with HIV cerebrospinal fluid (CSF) viral load in the context of opportunistic CNS infections are poorly understood. Our objective was to evaluate the relation between: (1) CSF HIV\u20101 viral load and CSF cytological and biochemical characteristics (leukocyte count, protein concentration, cryptococcal antigen titer); (2) CSF HIV\u20101 viral load and HIV\u20101 plasma viral load; and (3) CSF leukocyte count and the peripheral blood CD4+ T lymphocyte count.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Our approach was to use a prospective collection and analysis of pre\u2010treatment, paired CSF and plasma samples from antiretroviral\u2010naive HIV\u2010positive patients with cryptococcal meningitis and assisted at the Francisco J Mu\u00f1iz Hospital, Buenos Aires, Argentina (period: 2004 to 2006). We measured HIV CSF and plasma levels by polymerase chain reaction using the Cobas Amplicor HIV\u20101 Monitor Test version 1.5 (Roche). Data were processed with <jats:italic>Statistix<\/jats:italic> 7.0 software (linear regression analysis).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Samples from 34 patients were analyzed. CSF leukocyte count showed statistically significant correlation with CSF HIV\u20101 viral load (<jats:italic>r<\/jats:italic> = 0.4, 95% CI = 0.13\u20100.63, <jats:italic>p<\/jats:italic> = 0.01). No correlation was found with the plasma viral load, CSF protein concentration and cryptococcal antigen titer. A positive correlation was found between peripheral blood CD4+ T lymphocyte count and the CSF leukocyte count (<jats:italic>r<\/jats:italic> = 0.44, 95% CI = 0.125\u20100.674, <jats:italic>p<\/jats:italic> = 0.0123).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggests that CSF leukocyte count influences CSF HIV\u20101 viral load in patients with meningitis caused by <jats:italic>Cryptococcus neoformans<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1758-2652-12-33","type":"journal-article","created":{"date-parts":[[2009,11,26]],"date-time":"2009-11-26T00:01:20Z","timestamp":1259193680000},"page":"33-33","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Variables that influence HIV\u20101 cerebrospinal fluid viral load in cryptococcal meningitis: a linear regression analysis"],"prefix":"10.1002","volume":"12","author":[{"given":"Diego M","family":"Cecchini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M","family":"Ca\u00f1izal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haroldo","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Arechavala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Negroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda B","family":"Bouzas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A","family":"Benetucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,11]]},"reference":[{"issue":"2","key":"e_1_2_9_2_1","first-page":"115","article-title":"Kidney and central nervous system as reservoirs of HIV infection","volume":"1","author":"Fierer DS","year":"2006","journal-title":"Curr Opin HIV 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