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Multi-targeted PCR using three targets specific for <jats:italic>Mycobacterium tuberculosis<\/jats:italic>, i.e., <jats:italic>IS6110<\/jats:italic>, <jats:italic>MPB64<\/jats:italic>, and <jats:italic>protein b<\/jats:italic>, was performed on intraocular fluid samples of 25 subjects. Nine had presumed tubercular uveitis, six had intraocular inflammation secondary to a nontubercular etiology (disease controls), and ten had no evidence of intraocular inflammation (normal controls). As described previously, response to antitubercular therapy was considered as the gold standard.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Multi-targeted PCR was positive in seven out of nine patients with presumed tubercular uveitis and negative in all normal and disease controls. The sensitivity and specificity were 77.77% and 100%, respectively. For the diagnosis of presumed tubercular uveitis, multi-targeted PCR had a positive predictive value of 100% and a negative predictive value of 88.88%.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Multi-targeted PCR can be a valuable tool for diagnosing presumed tubercular uveitis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1869-5760-3-25","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T18:48:56Z","timestamp":1362077336000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":95,"title":["Novel multi-targeted polymerase chain reaction for diagnosis of presumed tubercular uveitis"],"prefix":"10.1186","volume":"3","author":[{"given":"Kusum","family":"Sharma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishali","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reema","family":"Bansal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aman","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meera","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amod","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,1,28]]},"reference":[{"key":"21_CR1","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.survophthal.2007.08.015","volume":"56","author":"V Gupta","year":"2007","unstructured":"Gupta V, Gupta A, Rao NA: Intraocular tuberculosis - an update. 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