{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:29:16Z","timestamp":1788496156861,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,11]],"date-time":"2012-12-11T00:00:00Z","timestamp":1355184000000},"content-version":"vor","delay-in-days":345,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational All"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2\u00a0in cross\u2010reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2\u00a0in cross\u2010reactivity among mustard, tree nuts and peanut.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eleven well\u2010characterized mustard\u2010allergic patients sensitized to Sin a 2 were included in the study. A specific anti\u2010Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT), enzyme\u2010linked immunosorbent assay (ELISA), immunoblotting and IgG or IgE\u2010inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The rabbit anti\u2010Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut) but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and\/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross\u2010reactivity among mustard, tree nuts and peanut as well as species\u2010specific IgE epitopes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The allergenic 11S globulin Sin a 2 from mustard is involved in cross\u2010reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross\u2010reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.<\/jats:p><\/jats:sec>","DOI":"10.1186\/2045-7022-2-23","type":"journal-article","created":{"date-parts":[[2012,12,11]],"date-time":"2012-12-11T17:14:21Z","timestamp":1355246061000},"source":"Crossref","is-referenced-by-count":27,"title":["The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross\u2010reactivity with homologous counterparts from tree nuts and peanut"],"prefix":"10.1002","volume":"2","author":[{"given":"Sof\u00eda","family":"Sirvent","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Chemistry Complutense University of Madrid 28040 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martial","family":"Akotenou","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Chemistry Complutense University of Madrid 28040 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Cuesta\u2010Herranz","sequence":"additional","affiliation":[{"name":"Service of Allergy IIS\u2010Fundaci\u00f3n Jim\u00e9nez D\u00edaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Vereda","sequence":"additional","affiliation":[{"name":"Service of Allergy IIS\u2010Fundaci\u00f3n Jim\u00e9nez D\u00edaz Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosal\u00eda","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Chemistry Complutense University of Madrid 28040 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayte","family":"Villalba","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Chemistry Complutense University of Madrid 28040 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Palomares","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology School of Chemistry Complutense University of Madrid 28040 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.02.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2010.582"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385989-1.00004-1"},{"key":"e_1_2_9_5_1","first-page":"317","article-title":"Mustard allergy. 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