{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:46:45Z","timestamp":1760820405166},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["AMB Expr"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortium, refining our previous observations of aerobic degradation. The aerobic consortium depleted, in decreasing order, hydrocarbons &gt; hopanes &gt; steranes &gt; tricyclic terpanes while the anaerobic consortium depleted hydrocarbons &gt; steranes &gt; hopanes &gt; tricyclic terpanes. The oxygen content of the mixed consortia was measured from time to time revealing alternating periods of microaerobicity (O<jats:sub>2<\/jats:sub> ~0.8 mg.L<jats:sup>-1<\/jats:sup>) and of aerobicity (O<jats:sub>2<\/jats:sub>~6.0 mg.L<jats:sup>-1<\/jats:sup>). In this experiment, the petroleum biodegradation changed from time to time, alternating periods of biodegradation similar to the aerobic process and periods of biodegradation similar to the anaerobic process. The consortia showed preferences for metabolizing hydrocarbons &gt; hopanes &gt; steranes &gt; tricyclic terpanes during a 90-day period, after which this trend changed and steranes were more biodegraded than hopanes. The analysis of aerobic oil degrading microbiota by the 16S rRNA gene clone library detected the presence of <jats:italic>Bacillus<\/jats:italic>, <jats:italic>Brevibacterium<\/jats:italic>, <jats:italic>Mesorhizobium<\/jats:italic> and <jats:italic>Achromobacter<\/jats:italic>, and the analysis of the anaerobic oil degrading microbiota using the same technique detected the presence of <jats:italic>Bacillus<\/jats:italic> and <jats:italic>Acinetobacter<\/jats:italic> (facultative strains). In the mixed consortia <jats:italic>Stenotrophomonas<\/jats:italic>, <jats:italic>Brevibacterium<\/jats:italic>, <jats:italic>Bacillus<\/jats:italic>, <jats:italic>Rhizobium<\/jats:italic>, <jats:italic>Achromobacter<\/jats:italic> and 5% uncultured bacteria were detected. This is certainly a new contribution to the study of reservoir biodegradation processes, combining two of the more important accepted hypotheses.<\/jats:p>","DOI":"10.1186\/2191-0855-1-47","type":"journal-article","created":{"date-parts":[[2011,12,23]],"date-time":"2011-12-23T19:16:30Z","timestamp":1324667790000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?"],"prefix":"10.1186","volume":"1","author":[{"given":"Georgiana F","family":"da Cruz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzan P","family":"de Vasconcellos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lio FF","family":"Angolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna M","family":"Dellagnezze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel NS","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria M","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenio V","family":"dos Santos Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita J","family":"Marsaioli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,12,23]]},"reference":[{"key":"43_CR1","first-page":"59","volume":"31","author":"M Balk","year":"2007","unstructured":"Balk M: Life in the absence of oxygen: alternative electron acceptors for anaerobic microorganisms in a petroleum environment. Turk J Biol 2007, 31: 59\u201366.","journal-title":"Turk J Biol"},{"key":"43_CR2","first-page":"305","volume":"47","author":"E Bieszkiewicz","year":"1998","unstructured":"Bieszkiewicz E, Horoch M, Boszczyk-Maleszak H, Mycielski R: An attempt to use selected strains of bacteria adapted to high concentrations of petroleum oil to increase the effective removal of petroleum products in excess activated sludge in laboratory conditions. Acta Microbiol Pol 1998, 47: 305\u2013312.","journal-title":"Acta Microbiol Pol"},{"key":"43_CR3","first-page":"82","volume":"75","author":"IA Borzenkov","year":"2006","unstructured":"Borzenkov IA, Milekhina ET, Gotoeva MT, Rozanova EP, Beliaev SS: The properties of hydrocarbon-oxidizing bacteria isolated from the oilfields of Tatarstan, Western Siberia, and Vietnam. Mikrobiologia 2006, 75: 82\u201389.","journal-title":"Mikrobiologia"},{"key":"43_CR4","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0146-6380(00)00147-9","volume":"32","author":"FD Bost","year":"2001","unstructured":"Bost FD, Frontera-Suau R, McDonald TJ, Peters KE, Morris PJ: Aerobic biodegradation of hopanes and norhopanes in Venezuelan crude oils. Org Geochem 2001, 32: 105\u2013114. 10.1016\/S0146-6380(00)00147-9","journal-title":"Org Geochem"},{"key":"43_CR5","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.resmic.2004.04.006","volume":"155","author":"F Chaillan","year":"2004","unstructured":"Chaillan F, Fl\u00e8che A, Bury E, Phantavong Y, Grimont P, Saliot A, Oudot J: Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms. Res Microbiol 2004, 155: 587\u2013595. 10.1016\/j.resmic.2004.04.006","journal-title":"Res Microbiol"},{"key":"43_CR6","doi-asserted-by":"publisher","first-page":"1724","DOI":"10.1002\/1615-9861(200212)2:12<1724::AID-PROT1724>3.0.CO;2-S","volume":"2","author":"LD Clements","year":"2002","unstructured":"Clements LD, Streips UN, Miller BS: Differential proteomic analysis of Bacillus subtilis nitrate respiration and fermentation in defined medium. Proteomics 2002, 2: 1724\u20131734. 10.1002\/1615-9861(200212)2:12<1724::AID-PROT1724>3.0.CO;2-S","journal-title":"Proteomics"},{"key":"43_CR7","doi-asserted-by":"crossref","first-page":"5234","DOI":"10.1128\/AEM.65.12.5234-5241.1999","volume":"65","author":"JD Coates","year":"1999","unstructured":"Coates JD: Ubiquity and diversity of disimilatory (per)chlorate-reducing bacteria. Appl Environ Microbiol 1999, 65: 5234\u20135241.","journal-title":"Appl Environ Microbiol"},{"key":"43_CR8","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/B978-0-12-032001-1.50011-0","volume-title":"Advances in Petroleum Geochemistry","author":"J Connan","year":"1984","unstructured":"Connan J: Biodegradation of crude oils in reservoirs. In Advances in Petroleum Geochemistry. Edited by: Brooks J, Welte DH. Academic Press, London; 1984:299\u2013330."},{"key":"43_CR9","doi-asserted-by":"publisher","first-page":"97","DOI":"10.2118\/13554-PA","volume":"39","author":"R Cord-Ruwich","year":"1987","unstructured":"Cord-Ruwich R, Kleinitz W, Widdel F: Sulphate-reducing bacteria and their activities in oil production. J Petrol Technol 1987, 39: 97\u2013106.","journal-title":"J Petrol Technol"},{"key":"43_CR10","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1007\/s00253-006-0531-2","volume":"73","author":"CD Cunha","year":"2006","unstructured":"Cunha CD, Rosado AS, Sebasti\u00e1n GV, Seldin L, Weid I: Oil biodegradation by Bacillus strains isolated from the rock of an oil reservoir located in a deep-water production basin in Brazil. Appl Microbiol Biotechnol 2006, 73: 949\u2013959. 10.1007\/s00253-006-0531-2","journal-title":"Appl Microbiol Biotechnol"},{"key":"43_CR11","doi-asserted-by":"publisher","first-page":"1204","DOI":"10.1016\/j.orggeochem.2008.04.010","volume":"39","author":"GF da Cruz","year":"2008","unstructured":"da Cruz GF, Santos Neto EV, Marsaioli AJ: Petroleum degradation by aerobic microbiota from the Pampo Sul Oil Field, Campos Basin, Brazil. Org Geochem 2008, 39: 1204\u20131209. 10.1016\/j.orggeochem.2008.04.010","journal-title":"Org Geochem"},{"issue":"8","key":"43_CR12","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1590\/S0103-50532010000800016","volume":"21","author":"GF da Cruz","year":"2010","unstructured":"da Cruz GF, Angolini CFF, Santos Neto EV, Loh W, Marsaioli AJ: Exopolymeric Substances (EPS) Produced by Petroleum Microbial Consortia. J Braz Chem Soc 2010,21(8):1517\u20131523. 10.1590\/S0103-50532010000800016","journal-title":"J Braz Chem Soc"},{"key":"43_CR13","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/S1369-5274(00)00085-0","volume":"3","author":"RL Derek","year":"2000","unstructured":"Derek RL, Coates JD: Novel forms of anaerobic respiration of environmental relevance. Curr Opin Microbiol 2000, 3: 252\u2013256. 10.1016\/S1369-5274(00)00085-0","journal-title":"Curr Opin Microbiol"},{"key":"43_CR14","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1101\/gr.8.3.175","volume":"8","author":"B Ewing","year":"1998","unstructured":"Ewing B, Hillier L, Wendl M, Green P: Base-calling of automated sequencer traces using phred. I Accuracy assessment Genome Res 1998, 8: 175\u2013185.","journal-title":"I Accuracy assessment Genome Res"},{"key":"43_CR15","doi-asserted-by":"crossref","first-page":"2802","DOI":"10.1128\/aem.63.7.2802-2813.1997","volume":"63","author":"JJ Godon","year":"1997","unstructured":"Godon JJ, Zumstein E, Dabert P, Habouzit F, Moletta R: Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Appl Environ Microbiol 1997, 63: 2802\u20132813.","journal-title":"Appl Environ Microbiol"},{"key":"43_CR16","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1093\/biomet\/40.3-4.237","volume":"40","author":"IJ Good","year":"1953","unstructured":"Good IJ: The population frequencies of species and the estimation of population parameters. Biometrika 1953, 40: 237\u2013264.","journal-title":"Biometrika"},{"key":"43_CR17","first-page":"650","volume-title":"Advances in Organic Geochemistry 1981","author":"NS Goodwin","year":"1983","unstructured":"Goodwin NS, Park PJD, Rawlinson T: Crude oil biodegradation. In Advances in Organic Geochemistry 1981. Edited by: Bjoroy M, Albrecht C, Cornford C, et al. John Wiley & Sons, New York; 1983:650\u2013658."},{"key":"43_CR18","volume-title":"EcoSim: Null models software for ecology","author":"NJ Gotelli","year":"2003","unstructured":"Gotelli NJ, Entsminger GL: EcoSim: Null models software for ecology. Volume VT 05465. Acquired Intelligence Inc. & Kesey-Bear, Burlington; 2003."},{"key":"43_CR19","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1128\/AEM.64.3.960-969.1998","volume":"64","author":"R Gro\u03b2kopf","year":"1998","unstructured":"Gro\u03b2kopf R, Stubner S, Liesack W: Novel Euryarchaeota lineages detected on rice roots and in the anoxic bulk soil of flooded rice microcosms. Appl Environ Microbiol 1998, 64: 960\u2013969.","journal-title":"Appl Environ Microbiol"},{"key":"43_CR20","first-page":"317","volume-title":"Petroleum Systems of South Atlantic Margins","author":"LR Guardado","year":"2000","unstructured":"Guardado LR, Spadini AR, Brand\u00e3o JSL, Mello MR: Petroleum system of the Campos Basin, Brazil. In Petroleum Systems of South Atlantic Margins. Volume 22. Edited by: Mello MR, Katz BJ. American Asssociation of Petroleum Geologists, Memoir 73; 2000:317\u2013324."},{"key":"43_CR21","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1038\/nature02134","volume":"426","author":"IM Head","year":"2003","unstructured":"Head IM, Jones DM, Larter SR: Biological activity in the deep subsurface and the origin of heavy oil. Nature 2003, 426: 344\u2013352. 10.1038\/nature02134","journal-title":"Nature"},{"issue":"1","key":"43_CR22","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.orggeochem.2009.07.004","volume":"41","author":"VB Heuer","year":"2010","unstructured":"Heuer VB, Kr\u00fcger M, Elvert M, Hinrichs K-U: Experimental studies on the stable carbon isotope biogeochemistry of acetate in lake sediments. Org Geochem 2010,41(1):22\u201330. 10.1016\/j.orggeochem.2009.07.004","journal-title":"Org Geochem"},{"key":"43_CR23","first-page":"600","volume-title":"Extended abstracts volume","author":"R Jahnert","year":"1998","unstructured":"Jahnert R, Fran\u00e7a A, Trindade LAF, Quintaes C, Santos P, Pessoa J, Bedregal RP: The petroleum system of Campos Basin. BGP; AAPG International Conference & Exhibition, November 8\u201311, Rio de Janeiro, Brasil. Extended abstracts volume 1998, 600\u2013601."},{"key":"43_CR24","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/nature06484","volume":"451","author":"DM Jones","year":"2008","unstructured":"Jones DM, Head IM, Gray ND, Adams JJ, Rowan AK, Aitken CM, Bennett B, Huang H, Brown A, Bowler BFJ, Oldenburg T, Erdmann M, Larter SR: Crude-oil biodegradation via methanogenesis in subsurface petroleum reservoirs. Nat Lett 2008, 451: 176\u2013181. 10.1038\/nature06484","journal-title":"Nat Lett"},{"key":"43_CR25","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/BF01731581","volume":"16","author":"M Kimura","year":"1980","unstructured":"Kimura M: A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980, 16: 111\u2013120. 10.1007\/BF01731581","journal-title":"J Mol Evol"},{"key":"43_CR26","first-page":"115","volume-title":"Nucleic Acid Techniques in Bacterial Systematics","author":"DJ Lane","year":"1991","unstructured":"Lane DJ: 16S\/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics. Edited by: Goodfellow M, Stackebrandt E. John Wiley and Sons, Chichester; 1991:115\u2013147."},{"key":"43_CR27","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1023\/A:1002434330514","volume":"77","author":"M Magot","year":"2000","unstructured":"Magot M, Ollivier B, Patel BKC: Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 2000, 77: 103\u2013116. 10.1023\/A:1002434330514","journal-title":"Antonie van Leeuwenhoek"},{"key":"43_CR28","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/0264-8172(88)90002-5","volume":"5","author":"MR Mello","year":"1988","unstructured":"Mello MR, Gaglianone PC, Brassell SC, Maxwell JR: Geochemical and biological marker assessment of depositional environments using Brazilian offshore oils. Marine Petrol Geol 1988, 5: 205\u2013223. 10.1016\/0264-8172(88)90002-5","journal-title":"Marine Petrol Geol"},{"issue":"38","key":"43_CR29","first-page":"577","volume":"46","author":"WU Mohriak","year":"1989","unstructured":"Mohriak WU, Mello MR, Karner GD, Dewey JF, Maxwell JR: Structural and stratigraphic evolution of the Campos Basin, Offshore Brazil. Extentional Tectonics and Stratigraphy of North Atlantic Margins, American Asssociation of Petroleum Geologists, Memoir 1989,46(38):577\u2013598.","journal-title":"Extentional Tectonics and Stratigraphy of North Atlantic Margins, American Asssociation of Petroleum Geologists, Memoir"},{"key":"43_CR30","doi-asserted-by":"publisher","first-page":"1891","DOI":"10.1016\/0016-7037(95)00072-8","volume":"59","author":"JM Moldowan","year":"1995","unstructured":"Moldowan JM, McCaffrey MA: A novel microbial hydrocarbon degradation pathway revealed by hopane demethylation in a petroleum reservoir. Geochim Cosmochim Acta 1995, 59: 1891\u20131894. 10.1016\/0016-7037(95)00072-8","journal-title":"Geochim Cosmochim Acta"},{"key":"43_CR31","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1016\/S0025-326X(97)00061-1","volume":"34","author":"D Munoz","year":"1997","unstructured":"Munoz D, Guiliano M, Doumenq P, Jacquot F, Scherrer P, Mille G: Long term evolution of petroleum biomarkers in mangrove soil. Marine Pollut Bull 1997, 34: 868\u2013874. 10.1016\/S0025-326X(97)00061-1","journal-title":"Marine Pollut Bull"},{"key":"43_CR32","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.anaerobe.2006.02.001","volume":"12","author":"I Neria-Gonz\u00e1les","year":"2006","unstructured":"Neria-Gonz\u00e1les I, Wang ET, Ram\u00edrez F, Romero JM, Hern\u00e1ndez-Rodr\u00edguez C: Characterization of bacterial community associated to biofilms of corroded oil pipelines from southeast of Mexico. Anaerobe 2006, 12: 122\u2013133. 10.1016\/j.anaerobe.2006.02.001","journal-title":"Anaerobe"},{"key":"43_CR33","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1007\/s10532-007-9104-z","volume":"18","author":"H Nestler","year":"2007","unstructured":"Nestler H, Kiesel B, Kaschabek SR, Mau M, Schl\u00f6man M, Balcke GU: Biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions. Biodegradation 2007, 18: 755\u2013767. 10.1007\/s10532-007-9104-z","journal-title":"Biodegradation"},{"key":"43_CR34","doi-asserted-by":"publisher","first-page":"307","DOI":"10.2113\/48.4.307","volume":"48","author":"M Obermajer","year":"2000","unstructured":"Obermajer M, Fowler MG, Snowdon LR, Macqueen RW: Compositional variability of crude oils and source kerogen in the Silurian carbonate-evaporite sequences of the eastern Michigan Basin, Ontario, Canada. Bull Can Petrol Geol 2000, 48: 307\u2013322. 10.2113\/48.4.307","journal-title":"Bull Can Petrol Geol"},{"key":"43_CR35","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1128\/AEM.66.2.700-711.2000","volume":"66","author":"VJ Orphan","year":"2000","unstructured":"Orphan VJ, Taylor LT, Hafenbradl D, Delong EF: Culture-dependent and culture-independent characterization of microbial assemblage associated with high temperature petroleum reservoirs. Appl Environ Microbiol 2000, 66: 700\u2013711. 10.1128\/AEM.66.2.700-711.2000","journal-title":"Appl Environ Microbiol"},{"key":"43_CR36","first-page":"511","volume-title":"Effects of biodegradtion and water washing on crude oil composition","author":"SE Palmer","year":"1993","unstructured":"Palmer SE: Effects of biodegradtion and water washing on crude oil composition. Edited by: Engel MH, Macko SA. Organic Geochemistry Plenum Press, New York; 1993:511\u2013533."},{"key":"43_CR37","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0146-6380(91)90039-M","volume":"17","author":"KE Peters","year":"1991","unstructured":"Peters KE, Moldowan JM: Effects of source, thermal maturity, and biodegradation on the distribuition and isomerization of homohopanes in petroleum. Org Geochem 1991, 17: 47\u201361. 10.1016\/0146-6380(91)90039-M","journal-title":"Org Geochem"},{"key":"43_CR38","volume-title":"Interpreting Molecular Fossils in petroleum and Ancient Sediments","author":"KE Peters","year":"1993","unstructured":"Peters KE, Moldowan JM: The Biomarker Guide. In Interpreting Molecular Fossils in petroleum and Ancient Sediments. Prentice-Hall, Englewood Cliffs, NJ; 1993."},{"key":"43_CR39","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1099\/00207713-46-3-759","volume":"46","author":"B Petterson","year":"1996","unstructured":"Petterson B, Lembke F, Hammer P, Stackebrandt E, Priest FG: Bacillus sporothermodurans , new species producing highly resistant endospores. Inter J System Bacteriol 1996, 46: 759\u2013764. 10.1099\/00207713-46-3-759","journal-title":"Inter J System Bacteriol"},{"key":"43_CR40","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1023\/B:BIOD.0000026476.03744.bb","volume":"15","author":"G Pineda-Flores","year":"2004","unstructured":"Pineda-Flores G, Boll-Arguello G, Lira-Galeana C, Mesta-Howard AM: A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation 2004, 15: 145\u2013151.","journal-title":"Biodegradation"},{"key":"43_CR41","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/S0960-8524(02)00119-0","volume":"85","author":"KSM Rahman","year":"2002","unstructured":"Rahman KSM, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat IM: Towards efficient crude oil degradation by a mixed bacterial consortium. Biores Technol 2002, 85: 257\u2013261. 10.1016\/S0960-8524(02)00119-0","journal-title":"Biores Technol"},{"key":"43_CR42","first-page":"203","volume":"8","author":"HD Rangel","year":"1994","unstructured":"Rangel HD, Martins FAL, Esteves FR, Feij\u00f3 FJ: Bacia de Campos. Bol Geoci\u00eanc Petrobr\u00e1s 1994, 8: 203\u2013217.","journal-title":"Bol Geoci\u00eanc Petrobr\u00e1s"},{"key":"43_CR43","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/S0923-2508(03)00086-X","volume":"154","author":"WFM Roling","year":"2003","unstructured":"Roling WFM, Head IM, Larter SR: The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs: perspectives and prospects. Res Microbiol 2003, 154: 321\u2013328. 10.1016\/S0923-2508(03)00086-X","journal-title":"Res Microbiol"},{"key":"43_CR44","first-page":"406","volume":"4","author":"N Saitou","year":"1987","unstructured":"Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4: 406\u2013425.","journal-title":"Mol Biol Evol"},{"key":"43_CR45","volume-title":"Msc Thesis","author":"GV Sebasti\u00e1n","year":"1999","unstructured":"Sebasti\u00e1n GV: Avalia\u00e7\u00e3o da popula\u00e7\u00e3o bacteriana presente em um reservat\u00f3rio de petr\u00f3leo situado em \u00e1guas profundas brasileiras, com \u00eanfase no isolamento e caracteriza\u00e7\u00e3o de estirpes de Bacillus . In Msc Thesis. Universidade Federal do Rio de Janeiro (RJ), Brazil; 1999."},{"key":"43_CR46","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/s10482-006-9115-5","volume":"91","author":"LD Sette","year":"2007","unstructured":"Sette LD, Simioni KCM, Vasconcellos SP, Dussan LJ, Neto EV, Oliveira VM: Analysis of the composition of bacterial communities in oil reservoirs from a southern offshore Brazilian basin. Antoine van Leewenhoek 2007, 91: 253\u2013266. 10.1007\/s10482-006-9115-5","journal-title":"Antoine van Leewenhoek"},{"key":"43_CR47","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1002\/(SICI)1097-0290(19960320)49:6<611::AID-BIT2>3.3.CO;2-Z","volume":"49","author":"CF Shen","year":"1996","unstructured":"Shen CF, Guiot SR: Long-term impact of dissolved O2 on the activity of anaerobic granules. Biotechnol Bioeng 1996, 49: 611\u2013620. 10.1002\/(SICI)1097-0290(19960320)49:6<611::AID-BIT2>3.3.CO;2-Z","journal-title":"Biotechnol Bioeng"},{"key":"43_CR48","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1093\/molbev\/msm092","volume":"24","author":"K Tamura","year":"2007","unstructured":"Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular evolutionary genetics analysis (MEGA) software, version 4.0. Mol Biol Evol 2007, 24: 1596\u20131599. 10.1093\/molbev\/msm092","journal-title":"Mol Biol Evol"},{"key":"43_CR49","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/S0146-6380(00)00176-5","volume":"32","author":"P Taylor","year":"2001","unstructured":"Taylor P, Bennett B, Jones M, Larter S: The effect of biodegradation and water washing on the occurrence of alkylphenols in crude oils. Org Geochem 2001, 32: 341\u2013358. 10.1016\/S0146-6380(00)00176-5","journal-title":"Org Geochem"},{"key":"43_CR50","doi-asserted-by":"publisher","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","volume":"24","author":"JD Thompson","year":"1997","unstructured":"Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997, 24: 4876\u20134882.","journal-title":"Nucleic Acids Res"},{"key":"43_CR51","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1128\/aem.62.5.1623-1629.1996","volume":"62","author":"G Voordouw","year":"1996","unstructured":"Voordouw G, Armstrong SM, Reimer MF, Fouts B, Telang AJ, Shen Y, Gevertz D: Characterization of 16S rRNA genes from oil field microbial communities indicates the presence of a variety of sulfate-reducing, fermentative, and sulfide-oxidizing bacteria. Appl Environ Microbiol 1996, 62: 1623\u20131629.","journal-title":"Appl Environ Microbiol"},{"key":"43_CR52","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/S0146-6380(99)00147-3","volume":"31","author":"H Wilkes","year":"2000","unstructured":"Wilkes H, Boreham C, Harms G, Zengler K, Rabus R: Anaerobic degradation and carbon isotopic fractionation of alkylbenzenes in crude oil by sulphate-reducing bacteria. Org Geochem 2000, 31: 101\u2013115. 10.1016\/S0146-6380(99)00147-3","journal-title":"Org Geochem"},{"key":"43_CR53","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/j.watres.2003.10.017","volume":"38","author":"J Xu","year":"2004","unstructured":"Xu J, Trimble JJ, Steinberg L, Logan BE: Chlorate and nitrate reduction pathways are separately induced in the perchlorate-respiring bacterium Dechlorosoma sp. KJ and the chlorate-respiring bacterium Pseudomonas sp . PDA. Water Res 2004, 38: 673\u2013680. 10.1016\/j.watres.2003.10.017","journal-title":"Water Res"},{"key":"43_CR54","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1038\/45777","volume":"401","author":"K Zengler","year":"1999","unstructured":"Zengler K, Richnow HH, Rosello-Mora R, Michaelis W, Widdel F: Methane formation from long-chain alkanes by anaerobic microorganisms. Nature 1999, 401: 266\u2013269. 10.1038\/45777","journal-title":"Nature"},{"key":"43_CR55","first-page":"944","volume-title":"Appl Environ Micorbiol","author":"J Zeyer","year":"1986","unstructured":"Zeyer J, Kuhn EP, Schawarzenbach PR: Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl Environ Micorbiol 1986, 944\u2013947."},{"key":"43_CR56","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1128\/aem.47.4.796-807.1984","volume":"47","author":"SH Zinder","year":"1984","unstructured":"Zinder SH, Cardwell SC, Anguish T, Lee M, Koch M: Methanogenesis in a thermophilic anaerobic digester: Methanotrix sp . as an important aceticlastic methanogen. Appl Environ Microbiol 1984, 47: 796\u2013807.","journal-title":"Appl Environ Microbiol"}],"container-title":["AMB Express"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/2191-0855-1-47.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T18:11:41Z","timestamp":1630519901000},"score":1,"resource":{"primary":{"URL":"https:\/\/amb-express.springeropen.com\/articles\/10.1186\/2191-0855-1-47"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["43"],"URL":"https:\/\/doi.org\/10.1186\/2191-0855-1-47","relation":{},"ISSN":["2191-0855"],"issn-type":[{"value":"2191-0855","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]},"assertion":[{"value":"20 October 2011","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 December 2011","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 December 2011","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"47"}}