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In the present study,<jats:italic>fliC<\/jats:italic>gene was cloned to study its biological implications. The recombinant FliC (rFliC) protein was observed to stimulate the production of antibodies. These antibodies could also recognize the FliC protein (antigen) in the clinical samples i.e. blood samples from typhoid patents as well as healthy blood samples spiked with serovar Typhi. Moreover, the rFliC also reacted with the sera from patients suffering with typhoid fever indicating its<jats:italic>in-vivo<\/jats:italic>immunogenicity.<jats:italic>Ex-vivo<\/jats:italic>study revealed that rFliC has the potential to stimulate the macrophages to generate higher levels of inflammatory mediators such as malondialdehyde (MDA) and nitrite. The inflammatory potential of FliC was also confirmed<jats:italic>in-vivo<\/jats:italic>, by the paw oedema test as well as by flicking response of the inflamed paw indicating hyperalgesia occurring during inflammatory response. The findings of the present study indicate that acid induced FliC might be one of the factors enhancing the virulence of serovar Typhi under the host acidic conditions and may prove to be helpful in designing the prophylactic measures.<\/jats:p>","DOI":"10.1186\/2191-0855-2-55","type":"journal-article","created":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T17:30:38Z","timestamp":1350322238000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Immunological characterization of recombinant Salmonella enterica serovar Typhi FliC protein expressed in Escherichia coli"],"prefix":"10.1186","volume":"2","author":[{"given":"Gaurav","family":"Jindal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupinder","family":"Tewari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankur","family":"Gautam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish K","family":"Pandey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Praveen","family":"Rishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,10,15]]},"reference":[{"key":"96_CR1","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1128\/JB.180.9.2409-2417.1998","volume":"180","author":"BL Bearson","year":"1998","unstructured":"Bearson BL, Wilson L, Foster JW: A low pH inducible, Pho P\/Q dependent acid tolerance response protects Salmonella Typhimurium against inorganic acid stress. 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