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This analysis revealed that NS5A protein represents the most probable interactor of M3R or that this viral protein could elicit antibodies which modulate function of this receptor. Further detailed structure\/function analysis of NS5A and M3R performed by the ISM method extended with other Digital Signal processing (DSP) approaches revealed domains of these proteins which participate in their crossreactivity or in their direct interaction, representing promising diagnostic and therapeutic targets.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Application of the ISM with other compatible bioinformatics methods offers new perspectives for identifying diagnostic and therapeutic targets for complicated forms of HCV and other viral infections. We show how the electron-ion interaction potential (EIIP) amino-acid scale used in the ISM combined with a robust, high performance hydrophobicity scale can provide new insights for understanding protein structure\/function and protein-protein interactions.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-016-0988-7","type":"journal-article","created":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T03:44:40Z","timestamp":1458618280000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Common molecular mechanism of the hepatic lesion and the cardiac parasympathetic regulation in chronic hepatitis C infection: a critical role for the muscarinic receptor type 3"],"prefix":"10.1186","volume":"17","author":[{"given":"Sanja","family":"Gli\u0161i\u0107","sequence":"first","affiliation":[]},{"given":"David P.","family":"Cavanaugh","sequence":"additional","affiliation":[]},{"given":"Krishnan K.","family":"Chittur","sequence":"additional","affiliation":[]},{"given":"Milan","family":"Sencanski","sequence":"additional","affiliation":[]},{"given":"Vladimir","family":"Perovic","sequence":"additional","affiliation":[]},{"given":"Tijana","family":"Boji\u0107","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,3,22]]},"reference":[{"issue":"1","key":"988_CR1","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1002\/bip.20607","volume":"85","author":"KA Selz","year":"2007","unstructured":"Selz KA, Samoylova TI, Samoylov AM, Vodyanoy VJ, Mandell AJ. 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