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Nonetheless, constructing the <jats:sup>13<\/jats:sup>C-MFA model and performing flux calculation are demanding for new learners, because they require knowledge of metabolic networks, carbon transitions, and computer programming. To facilitate and standardize the <jats:sup>13<\/jats:sup>C-MFA modeling work, we set out to publish a user-friendly and programming-free platform (WUFlux) for flux calculations in MATLAB<jats:sup>\u00ae<\/jats:sup>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We constructed an open-source platform for steady-state <jats:sup>13<\/jats:sup>C-MFA. Using GUIDE (graphical user interface design environment) in MATLAB, we built a user interface that allows users to modify models based on their own experimental conditions. WUFlux is capable of directly correcting mass spectrum data of TBDMS (N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide)-derivatized proteinogenic amino acids by removing background noise. To simplify <jats:sup>13<\/jats:sup>C-MFA of different prokaryotic species, the software provides several metabolic network templates, including those for chemoheterotrophic bacteria and mixotrophic cyanobacteria. Users can modify the network and constraints, and then analyze the microbial carbon and energy metabolisms of various carbon substrates (e.g., glucose, pyruvate\/lactate, acetate, xylose, and glycerol). WUFlux also offers several ways of visualizing the flux results with respect to the constructed network. To validate our model\u2019s applicability, we have compared and discussed the flux results obtained from WUFlux and other MFA software. We have also illustrated how model constraints of cofactor and ATP balances influence fluxome results.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Open-source software for <jats:sup>13<\/jats:sup>C-MFA, WUFlux, with a user-friendly interface and easy-to-modify templates, is now available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.13cmfa.org\">http:\/\/www.13cmfa.org<\/jats:ext-link>\/or (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/tang.eece.wustl.edu\/ToolDevelopment.htm\">http:\/\/tang.eece.wustl.edu\/ToolDevelopment.htm<\/jats:ext-link>). We will continue documenting curated models of non-model microbial species and improving WUFlux performance.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-016-1314-0","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T04:57:28Z","timestamp":1478235448000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["WUFlux: an open-source platform for 13C metabolic flux analysis of bacterial metabolism"],"prefix":"10.1186","volume":"17","author":[{"given":"Lian","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen G.","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinjie J.","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,4]]},"reference":[{"issue":"1","key":"1314_CR1","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1093\/bib\/bbs069","volume":"15","author":"M Lakshmanan","year":"2014","unstructured":"Lakshmanan M, Koh G, Chung BK, Lee D-Y. 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