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Computational tools that can visualize and aid the analysis of datasets by both techniques are in demand.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Raman2imzML<\/jats:italic>was developed as an open-source converter that transforms Raman imaging data into imzML, a standardized common data format created and adopted by the mass spectrometry community. We successfully converted Raman datasets to imzML and visualized Raman images using open-source software designed for MSI applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Raman2imzML<\/jats:italic>enables both MSI and Raman images to be visualized using the same file format and the same software for a straightforward exploratory imaging analysis.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12859-020-03789-8","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T12:02:59Z","timestamp":1602244979000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Raman2imzML converts Raman imaging data into the standard mass spectrometry imaging format"],"prefix":"10.1186","volume":"21","author":[{"given":"Stefania Alexandra","family":"Iakab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lluc","family":"Sement\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-1487","authenticated-orcid":false,"given":"Mar\u00eda","family":"Garc\u00eda-Altares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Correig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere","family":"R\u00e0fols","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"3789_CR1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1002\/mas.20124","volume":"26","author":"LA McDonnell","year":"2007","unstructured":"McDonnell LA, Heeren RMA. 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All procedures were approved by the University Rovira i Virgili Bioethics Committee, as stated in Law 5\/21 July 1995 passed by the Generalitat de Catalunya (Autonomous Government of Catalonia). No animals were sacrificed specifically for this work. The tissues used were unused in previous experiments.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"448"}}