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To extract useful information there is the need to extract spatial and temporal data on the particles that are in the images, but particle tracking and feature extraction need some kind of assistance.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>This manuscript introduces our new freely downloadable toolbox, the <jats:italic>Visual4DTracker<\/jats:italic>. It is a MATLAB package implementing several useful functionalities to navigate, analyse and proof-read the track of each particle detected in any <jats:inline-formula><jats:alternatives><jats:tex-math>$$3D+t$$<\/jats:tex-math><mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                    <mml:mrow>\n                      <mml:mn>3<\/mml:mn>\n                      <mml:mi>D<\/mml:mi>\n                      <mml:mo>+<\/mml:mo>\n                      <mml:mi>t<\/mml:mi>\n                    <\/mml:mrow>\n                  <\/mml:math><\/jats:alternatives><\/jats:inline-formula> stack. Furthermore, it allows users to proof-read and to evaluate the traces with respect to a given gold standard. The Visual4DTracker toolbox permits the users to visualize and save all the generated results through a user-friendly graphical user interface. This tool has been successfully used in three applicative examples. The first processes synthetic data to show all the software functionalities. The second shows how to process a 4<jats:italic>D<\/jats:italic> image stack showing the time-lapse growth of Drosophila cells in an embryo. The third example presents the quantitative analysis of insulin granules in living beta-cells, showing that such particles have two main dynamics that coexist inside the cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Visual4DTracker is a software package for MATLAB to visualize, handle and manually track <jats:inline-formula><jats:alternatives><jats:tex-math>$$3D+t$$<\/jats:tex-math><mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                    <mml:mrow>\n                      <mml:mn>3<\/mml:mn>\n                      <mml:mi>D<\/mml:mi>\n                      <mml:mo>+<\/mml:mo>\n                      <mml:mi>t<\/mml:mi>\n                    <\/mml:mrow>\n                  <\/mml:math><\/jats:alternatives><\/jats:inline-formula> stacks of microscopy images containing objects such cells, granules, etc.. With its unique set of functions, it remarkably permits the user to analyze and proof-read 4<jats:italic>D<\/jats:italic> data in a friendly 3<jats:italic>D<\/jats:italic> fashion. The tool is freely available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/drive.google.com\/drive\/folders\/19AEn0TqP-2B8Z10kOavEAopTUxsKUV73?usp=sharing\">https:\/\/drive.google.com\/drive\/folders\/19AEn0TqP-2B8Z10kOavEAopTUxsKUV73?usp=sharing<\/jats:ext-link><\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-020-03820-y","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T22:44:17Z","timestamp":1612824257000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Visual4DTracker: a tool to interact with 3D + t image stacks"],"prefix":"10.1186","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6062-7575","authenticated-orcid":false,"given":"Ermanno","family":"Cordelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Soda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulio","family":"Iannello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"issue":"6","key":"3820_CR1","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1242\/jcs.033837","volume":"122","author":"MM Frigault","year":"2009","unstructured":"Frigault MM, Lacoste J, Swift JL, Brown CM. 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