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However, current m<jats:sup>6<\/jats:sup>A profiles in public databases are not readily intercomparable, where heterogeneous profiles from the same experimental report but different cell types showed unwanted high correlations.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Several normalizing or correcting methods were tested to remove such laboratory bias. And m6Acorr, an effective pipeline for correcting m<jats:sup>6<\/jats:sup>A profiles, was presented on the basis of quantile normalization and empirical Bayes batch regression method. m6Acorr could efficiently correct laboratory bias in the simulated dataset and real m<jats:sup>6<\/jats:sup>A profiles in public databases. The preservation of biological signals was examined after correction, and m6Acorr was found to better preserve differential methylation signals, m<jats:sup>6<\/jats:sup>A regulated targets, and m<jats:sup>6<\/jats:sup>A-related biological features than alternative methods. Finally, the m6Acorr server was established. This server could eliminate the potential laboratory bias in m<jats:sup>6<\/jats:sup>A methylation profiles and perform profile\u2013profile comparisons and functional analysis of hyper- (hypo-) methylated genes based on corrected methylation profiles.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>m6Acorr was established to correct the existing laboratory bias in RNA m<jats:sup>6<\/jats:sup>A methylation profiles and perform profile comparisons on the corrected datasets. The m6Acorr server is available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.rnanut.net\/m6Acorr\">http:\/\/www.rnanut.net\/m6Acorr<\/jats:ext-link>. A stand-alone version with the correction function is also available in GitHub at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/github.com\/emersON106\/m6Acorr\">https:\/\/github.com\/emersON106\/m6Acorr<\/jats:ext-link>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12859-020-3380-6","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T10:03:06Z","timestamp":1580292186000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["m6Acorr: an online tool for the correction and comparison of m6A methylation profiles"],"prefix":"10.1186","volume":"21","author":[{"given":"Jianwei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghua","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5685-066X","authenticated-orcid":false,"given":"Yuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,1,29]]},"reference":[{"issue":"3","key":"3380_CR1","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1038\/nrm.2016.163","volume":"18","author":"CJ Lewis","year":"2017","unstructured":"Lewis CJ, Pan T, Kalsotra A. 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