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Oocyte appearance also changes with aging, suggesting a functional relationship between oocyte quality and appearance. However, no methods are available to objectively quantify age-associated changes in oocyte appearance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We show that statistical image processing of Nomarski differential interference contrast microscopy images can be used to quantify age-associated changes in oocyte appearance in the nematode\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                      . Max\u2013min value (mean difference between the maximum and minimum intensities within each moving window) quantitatively characterized the difference in oocyte cytoplasmic texture between 1- and 3-day-old adults (Day 1 and Day 3 oocytes, respectively). With an appropriate parameter set, the gray level co-occurrence matrix (GLCM)-based texture feature\n                      <jats:italic>Correlation<\/jats:italic>\n                      (COR) more sensitively characterized this difference than the Max\u2013min Value. Manipulating the smoothness of and\/or adding irregular structures to the cytoplasmic texture of Day 1 oocyte images reproduced the difference in Max\u2013min Value but not in COR between Day 1 and Day 3 oocytes. Increasing the size of granules in synthetic images recapitulated the age-associated changes in COR. Manual measurements validated that the cytoplasmic granules in oocytes become larger with aging.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The Max\u2013min value and COR objectively quantify age-related changes in\n                      <jats:italic>C. elegans<\/jats:italic>\n                      oocyte in Nomarski DIC microscopy images. Our methods provide new opportunities for understanding the mechanism underlying oocyte aging.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12859-021-03990-3","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T03:00:13Z","timestamp":1613617213000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Statistical image processing quantifies the changes in cytoplasmic texture associated with aging in Caenorhabditis elegans oocytes"],"prefix":"10.1186","volume":"22","author":[{"given":"Momoko","family":"Imakubo","sequence":"first","affiliation":[]},{"given":"Jun","family":"Takayama","sequence":"additional","affiliation":[]},{"given":"Hatsumi","family":"Okada","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-1724","authenticated-orcid":false,"given":"Shuichi","family":"Onami","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"3990_CR1","doi-asserted-by":"crossref","unstructured":"Navot D, Bergh PA, Williams MA, GArrisi GJ, Guzman I, Sandler B, et al. 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