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The 1000 Genomes Project recorded individual genotypes across 26 different populations and, using computerized genotype phasing, reported haplotype data. In contrast, we identified long reference sequences by analyzing the homozygous genomic regions in this online database, a concept that has rarely been reported since next generation sequencing data became available.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Study design and methods<\/jats:title>\n                <jats:p>Phased genotype data for a 80.6\u00a0kb region of chromosome 1 was downloaded for all 2,504 unrelated individuals of the 1000 Genome Project Phase 3 cohort. The data was centered on the <jats:italic>ACKR1<\/jats:italic> gene and bordered by the <jats:italic>CADM3<\/jats:italic> and <jats:italic>FCER1A<\/jats:italic> genes. Individuals with heterozygosity at a single site or with complete homozygosity allowed unambiguous assignment of an <jats:italic>ACKR1<\/jats:italic> haplotype. A computer algorithm was developed for extracting these haplotypes from the 1000 Genome Project in an automated fashion. A manual analysis validated the data extracted by the algorithm.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We confirmed 902 <jats:italic>ACKR1<\/jats:italic> haplotypes of varying lengths, the longest at 80,584 nucleotides and shortest at 1,901 nucleotides. The combined length of haplotype sequences comprised 19,895,388 nucleotides with a median of 16,014 nucleotides. Based on our approach, all haplotypes can be considered experimentally confirmed and not affected by the known errors of computerized genotype phasing.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Tracts of homozygosity can provide definitive reference sequences for any gene. They are particularly useful when observed in unrelated individuals of large scale sequence databases. As a proof of principle, we explored the 1000 Genomes Project database for <jats:italic>ACKR1<\/jats:italic> gene data and mined long haplotypes. These haplotypes are useful for high throughput analysis with next generation sequencing. Our approach is scalable, using automated bioinformatics tools, and can be applied to any gene.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-021-04169-6","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T11:05:02Z","timestamp":1622027102000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cataloguing experimentally confirmed 80.7\u00a0kb-long ACKR1 haplotypes from the 1000 Genomes Project database"],"prefix":"10.1186","volume":"22","author":[{"given":"Kshitij","family":"Srivastava","sequence":"first","affiliation":[]},{"given":"Anne-Sophie","family":"Fratzscher","sequence":"additional","affiliation":[]},{"given":"Bo","family":"Lan","sequence":"additional","affiliation":[]},{"given":"Willy Albert","family":"Flegel","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,5,26]]},"reference":[{"key":"4169_CR1","doi-asserted-by":"publisher","first-page":"D423","DOI":"10.1093\/nar\/gku1161","volume":"43","author":"J Robinson","year":"2015","unstructured":"Robinson J, et al. 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