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To maximize the performance, the parameters of the CNV calling algorithms should be optimized for each specific dataset. This requires obtaining validated CNV information using either multiplex ligation-dependent probe amplification (MLPA) or array comparative genomic hybridization (aCGH). They are gold standard but time-consuming and costly approaches.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We present isoCNV which optimizes the parameters of DECoN algorithm using only NGS data. The parameter optimization process is performed using an in silico CNV validated dataset obtained from the overlapping calls of three algorithms: CNVkit, panelcn.MOPS and DECoN. We evaluated the performance of our tool and showed that increases the sensitivity in both TS and WES real datasets.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>isoCNV provides an easy-to-use pipeline to optimize DECoN that allows the detection of analysis-ready CNV from a set of DNA alignments obtained under the same conditions. It increases the sensitivity of DECoN without the need for orthogonal methods. isoCNV is available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/gitlab.com\/sequentiateampublic\/isocnv\">https:\/\/gitlab.com\/sequentiateampublic\/isocnv<\/jats:ext-link>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-021-04452-6","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T11:02:59Z","timestamp":1635505379000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["isoCNV: in silico optimization of copy number variant detection from targeted or exome sequencing data"],"prefix":"10.1186","volume":"22","author":[{"given":"Rosa","family":"Barcelona-Cabeza","sequence":"first","affiliation":[]},{"given":"Walter","family":"Sanseverino","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9058-6994","authenticated-orcid":false,"given":"Riccardo","family":"Aiese Cigliano","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,10,29]]},"reference":[{"key":"4452_CR1","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1097\/JBR.0000000000000021","volume":"1","author":"L Huang","year":"2018","unstructured":"Huang L, Yang J, Xu S, Mao Y, Lee DY, Yang J, et al. 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