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Internal ribosomal entry site (IRES) and N<jats:sup>6<\/jats:sup>-methyladenosine (m<jats:sup>6<\/jats:sup>A) were reported to drive translation of circRNAs. Experimental methods confirming the presence of IRES and m<jats:sup>6<\/jats:sup>A site are time consuming and labor intensive. Lacking computational tools to predict ORFs, IRESs and m<jats:sup>6<\/jats:sup>A sites for circRNAs makes it harder.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In this report, we present circPrimer 2.0, a Java based software for annotating circRNAs and predicting ORFs, IRESs, and m6A sites of circRNAs. circPrimer 2.0 has a graphical and a command-line interface that enables the tool to be embed into an analysis pipeline.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>circprimer 2.0 is an easy-to-use software for annotating circRNAs and predicting translation potential of circRNAs, and freely available at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.bio-inf.cn\">www.bio-inf.cn<\/jats:ext-link>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-022-04705-y","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T07:03:53Z","timestamp":1654499033000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["CircPrimer 2.0: a software for annotating circRNAs and predicting translation potential of circRNAs"],"prefix":"10.1186","volume":"23","author":[{"given":"Shanliang","family":"Zhong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3541-2342","authenticated-orcid":false,"given":"Jifeng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"issue":"10","key":"4705_CR1","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1007\/s10529-019-02723-0","volume":"41","author":"S Zhong","year":"2019","unstructured":"Zhong S, Zhou S, Yang S, Yu X, Xu H, Wang J, Zhang Q, Lv M, Feng J. Identification of internal control genes for circular RNAs. Biotechnol Lett. 2019;41(10):1111\u20139.","journal-title":"Biotechnol Lett"},{"key":"4705_CR2","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.semcancer.2018.12.002","volume":"58","author":"E Arnaiz","year":"2019","unstructured":"Arnaiz E, Sole C, Manterola L, Iparraguirre L, Otaegui D, Lawrie CH. CircRNAs and cancer: Biomarkers and master regulators. Semin Cancer Biol. 2019;58:90\u20139.","journal-title":"Semin Cancer Biol"},{"issue":"1","key":"4705_CR3","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.molcel.2017.02.021","volume":"66","author":"NR Pamudurti","year":"2017","unstructured":"Pamudurti NR, Bartok O, Jens M, Ashwal-Fluss R, Stottmeister C, Ruhe L, Hanan M, Wyler E, Perez-Hernandez D, Ramberger E, et al. Translation of CircRNAs. 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