{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T23:11:56Z","timestamp":1773184316830,"version":"3.50.1"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100012390","name":"SystemsX.ch","doi-asserted-by":"publisher","award":["MRD 2014\/267"],"award-info":[{"award-number":["MRD 2014\/267"]}],"id":[{"id":"10.13039\/501100012390","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["Synergy Grant 609883"],"award-info":[{"award-number":["Synergy Grant 609883"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swiss Federal Institute of Technology Zurich"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Bioinformatics"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Liquid biopsy is a minimally-invasive method of sampling bodily fluids, capable of revealing evidence of cancer. The distribution of cell-free DNA (cfDNA) fragment lengths has been shown to differ between healthy subjects and cancer patients, whereby the distributional shift correlates with the sample\u2019s tumour content. These fragmentomic data have not yet been utilised to directly quantify the proportion of tumour-derived cfDNA in a liquid biopsy.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We used statistical learning to predict tumour content from Fourier and wavelet transforms of cfDNA length distributions in samples from 118 cancer patients. The model was validated on an independent dilution series of patient plasma.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This proof of concept suggests that our fragmentomic methodology could be useful for predicting tumour content in liquid biopsies.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12859-023-05478-8","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T14:01:37Z","timestamp":1696082497000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Predicting tumour content of liquid biopsies from cell-free DNA"],"prefix":"10.1186","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5873-2632","authenticated-orcid":false,"given":"Mathias","family":"Cardner","sequence":"first","affiliation":[]},{"given":"Francesco","family":"Marass","sequence":"additional","affiliation":[]},{"given":"Erika","family":"Gedvilaite","sequence":"additional","affiliation":[]},{"given":"Julie L.","family":"Yang","sequence":"additional","affiliation":[]},{"given":"Dana W. 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Ethics approval was previously granted to Tsui et al.\u00a0[], who originally published the data.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Unrelated to this work, MC is employed by AstraZeneca AB. FM and DWYT are employed by PetDx, Inc. EG, JLY, and NB have no competing interests to declare.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"368"}}